渗透胁迫下不同玉米品种幼苗ABA合成及生理生化响应差异

doi:10.3724/SP.J.1006.2026.53059

摘要/Abstract

摘要： 脱落酸(ABA)是响应干旱胁迫的重要逆境信号转导物质，但玉米品种干旱敏感性与ABA积累的内在关系仍不清晰。本研究以郑单958、农大364和农大3138为供试品种，采用聚乙二醇(PEG-6000)模拟渗透胁迫，比较研究渗透胁迫对玉米幼苗生长、ABA合成与积累及其生理生化特征的影响，探究ABA在不同玉米品种中响应渗透胁迫的作用机制。主要研究结果如下，渗透胁迫显著抑制不同品种幼苗地上部与根系的生长与生物量积累，降低了植株相对含水量。其中郑单958、农大3138和农大364的地上部和地下部生物量分别抑制32.8%和5.9%、37.1%和10.5%、43.8%和20.1%。同时，渗透胁迫显著上调了不同品种叶片和根系中ABA合成关键基因ZmNCED1、ZmAO2和ZmABA3的表达水平，其中郑单958增幅最大，较对照最大增加1.5~12.9倍，而农大364增幅最小，较对照仅增加0.4~1.3倍。渗透胁迫增强了ABA合成关键酶醛氧化酶活性，促进了ABA积累，其中农大364的叶片和根中ABA积累增幅最小，分别为140%和90%，而郑单958最大。此外，渗透胁迫显著提高了玉米抗氧化酶POD、SOD和CAT活性，不同品种间增幅与ABA含量的增幅规律一致。从不同品种的植株生长、ABA合成和积累相关生理生化特征、抗氧化酶活性等对渗透胁迫的响应特征进行综合分析，品种抗旱性表现为郑单958 >农大3138 >农大364。研究结果揭示了ABA在不同抗旱性玉米品种响应渗透胁迫中的作用机制，为抗旱品种选育和抗逆栽培措施构建提供依据。

关键词: 玉米, ABA, 渗透胁迫, 醛氧化酶, 抗氧化酶

Abstract:

Osmotic stress significantly inhibited the growth and biomass accumulation of both aboveground and belowground parts in seedlings of different maize cultivars, and reduced the relative water content (RWC) of the plants. Specifically, the aboveground and root biomass of Zhengdan 958, Nongda 3138, and Nongda 364 were reduced by 32.8% and 5.9%, 37.1% and 10.5%, and 43.8% and 20.1%, respectively. Meanwhile, drought stress markedly upregulated the expression of key ABA biosynthesis genes—including ZmNCED1, ZmAO2, and ZmABA3—in both leaves and roots across cultivars. Among them, Zhengdan 958 exhibited the greatest increase, with transcript levels rising 1.5- to 12.9-fold compared to the control, whereas Nongda 364 showed the smallest increase, ranging from 0.4- to 1.3-fold. Osmotic stress also enhanced the activity of aldehyde oxidase, a key enzyme in ABA biosynthesis, thereby promoting ABA accumulation. Among the cultivars, Nongda 364 exhibited the smallest ABA increase in leaves and roots (140% and 90%, respectively), while Zhengdan 958 showed the largest. In addition, osmotic stress significantly elevated the activities of antioxidant enzymes in maize, including POD, SOD, and CAT, with the degree of increase consistent with the trend of ABA accumulation among cultivars. A comprehensive analysis of plant growth, physiological and biochemical characteristics related to ABA biosynthesis and accumulation, and antioxidant enzyme activities under osmotic stress revealed that drought tolerance among the cultivars followed the order: Zhengdan 958 > Nongda 3138 > Nongda 364. These findings elucidate the role of ABA in mediating maize responses to osmotic stress in cultivars with varying drought tolerance and provide a theoretical foundation for breeding drought-tolerant cultivars and developing stress-resilient cultivation strategies.

Key words: maize, ABA, osmotic stress, aldehyde oxidase, antioxidant enzymes

张清怡, 肖义涛, 李秋霞, 张钰石, 张明才, 李召虎. 渗透胁迫下不同玉米品种幼苗ABA合成及生理生化响应差异[J]. 作物学报, 0, (): 0-.

Zhang Qing-Yi, Xiao Yi-Tao, Li Qiu-Xia, Zhang Yu-Shi, Zhang Ming-Cai, Li Zhao-Hu. Differences in ABA synthesis and physiological and biochemical responses of seedlings of different maize varieties under osmotic stress#br#

#br#

[J]. Acta Agronomica Sinica, 0, (): 0-.

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