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蒋环琪1,2,段奥1,郭超1,黄晓梦1,艾德骏1,刘小雪1,谭静怡1,彭成林2,李曼菲1,*,杜何为1,*
JIANG Huan-Qi1,2,DUAN Ao1,GUO Chao1,HUANG Xiao-Meng1,AI De-Jun1,LIU Xiao-Xue1,TAN Jing-Yi1,PENG Cheng-Lin²,LI Man-Fei1,*
摘要:
为探究玉米(Zea mays L.)幼苗根系在渍水胁迫下关键代谢物以及代谢途径的变化,阐明与渍水胁迫相关的代谢通路,对玉米幼苗进行0、1、4和7 d共4个渍水胁迫时间梯度处理,采用转录组学测序(RNA sequencing, RNA-seq)和超高效液相色谱质谱联用技术(ultra performance liquid chromatography tandem mass spectrometry, UPLC-MS/MS),筛选与渍水胁迫相关的关键代谢物,并通过KEGG富集分析探究相关代谢通路。结果显示,代谢组共标注和定量了1361种差异代谢物,渍水4 d时根系代谢物变化最为显著,共有414种代谢物发生变化,其中372种代谢物含量上升,42种代谢物含量下降;代谢物差异倍数排名前20的代谢物均呈现上调趋势,包括阿魏酸-4-O-葡萄糖苷、2-苯乙醇、7-甲基柚皮素和S-烯丙基-L-半胱氨酸等;差异显著代谢物的KEGG富集分析结果显示,黄酮类化合物生物合成、类胡萝卜素生物合成、苯丙素生物合成、植物激素信号转导、ABC转运蛋白、脂肪酸降解、淀粉和蔗糖代谢、甘氨酸、丝氨酸和苏氨酸代谢、色氨酸代谢以及糖酵解/糖异生等代谢途径中,差异基因与差异代谢物较为显著富集。类黄酮生物合成代谢通路与渍水胁迫密切相关,柚皮素与木犀草素等黄酮类物质,查尔酮异构酶、黄酮合成酶II与黄酮3',5'-羟化酶/黄酮3'-单氧化酶可能起关键作用。研究结果为探究玉米耐渍胁迫分子机制提供科学支撑与理论依据,为玉米耐渍育种等相关研究提供理论基础。
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