大豆百粒重稳定QTL qSW20-1定位及对产量和品质的影响

doi:10.3724/SP.J.1006.2023.34022

作物学报 ›› 2023, Vol. 49 ›› Issue (10): 2621-2632.doi: 10.3724/SP.J.1006.2023.34022

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

大豆百粒重稳定QTL qSW20-1定位及对产量和品质的影响

孙建强¹^,²(), 洪慧龙¹^,², 张勇³, 谷勇哲², 高华伟², 周雅², 曹杰², 祁航², 赵权², 包立高⁴, 陈庆山¹, 邱丽娟¹^,²()

¹东北农业大学农学院, 黑龙江哈尔滨 150030
²农作物基因资源与遗传改良国家重大科学工程 / 农业农村部种质资源利用重点实验室 / 中国农业科学院作物科学研究所, 北京 100081
³黑龙江省农业科学院克山分院, 黑龙江齐齐哈尔 161606
⁴内蒙古自治区农牧业技术推广中心, 内蒙古呼和浩特 010018

收稿日期:2023-02-04 接受日期:2023-04-17 出版日期:2023-10-12 网络出版日期:2023-04-26
通讯作者: 邱丽娟, E-mail: qiulijuan@caas.cn
作者简介:孙建强, E-mail: 1209506464@qq.com **同等贡献
基金资助:
本研究由国家重点研发计划项目(2021YFD1201600)

Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean

SUN Jian-Qiang¹^,²(), HONG Hui-Long¹^,², ZHANG Yong³, GU Yong-Zhe², GAO Hua-Wei², ZHOU Ya², CAO Jie², QI Hang², ZHAO Quan², BAO Li-Gao⁴, CHEN Qing-Shan¹, QIU Li-Juan¹^,²()

¹College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
²National Key Facility for Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture and Rural Affairs / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
³Keshan Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161606, Heilongjiang, China
⁴Agriculture and Animal Husbandry Technology Promotion Center of Inner Mongolia Autonomous Region, Hohhot 010018, Inner Mongolia, China

Received:2023-02-04 Accepted:2023-04-17 Published:2023-10-12 Published online:2023-04-26
Contact: E-mail: qiulijuan@caas.cn
About author:**Contributed equally to this work
Supported by:
National Key Research and Development Program of China(2021YFD1201600)

摘要/Abstract

摘要：

籽粒重量是大豆产量构成的关键因素之一, 克隆主要数量性状基因座(QTL)中控制种子重量的关键基因对提高大豆产量具有重要意义。本研究以齐黄34×东生16构建的325个重组自交系(RIL)为材料, 利用SLAF-seq构建了高密度遗传连锁图谱, 总图距为2945.26 cM, 平均图距为0.47 cM, 结合3个环境百粒重表型, 检测到11个与百粒重相关的QTL。其中, 环境稳定的QTL为qSW20-1, 可解释9.73%~18.10%的表型变异。该QTL的大粒等位基因可显著增加单株粒数和单株粒重, 但对蛋白含量和脂肪含量2个品质性状无不利影响, 区间大小为435.42 kb, 包含36个基因, 通过基因注释和表达模式分析, 预测5个候选基因。本研究结果为大豆增产基因挖掘和分子设计育种奠定了坚实的基础。

关键词: 大豆, 百粒重, SLAF图谱, QTL定位

Abstract:

Seed weight is one of the key factors of soybean yield. Cloning the key genes controlling seed weight in major quantitative trait loci (QTL) is of great significance to improve soybean yield. In this study, a high-density genetic linkage map was constructed by SLAF-seq using 325 recombinant inbred lines (RILs) constructed by Qihuang 34 × Dongsheng 16 as the experimental materials. The total map distance was 2945.26 cM, and the average map distance was 0.47 cM. Combined with three environmental 100-seed weight phenotypes, 11 QTL related to 100-seed weight were detected. Among them, the QTL with stable environment was qSW20-1, which explaining 9.73%-18.10% of phenotypic variation. The large grain allele of the QTL could significantly increase the number of seed per plant and seed weight per plant, but there was no adverse effect on the two quality traits of protein content and fat content. The interval size was 435.42 kb, containing 36 genes. Five candidate genes were predicted by gene annotation and expression pattern analysis. The results of this study laid a solid foundation for soybean yield-increasing gene mining and molecular design breeding.

Key words: soybean, 100-seed weight, SLAF map, QTL mapping

孙建强, 洪慧龙, 张勇, 谷勇哲, 高华伟, 周雅, 曹杰, 祁航, 赵权, 包立高, 陈庆山, 邱丽娟. 大豆百粒重稳定QTL qSW20-1定位及对产量和品质的影响[J]. 作物学报, 2023, 49(10): 2621-2632.

SUN Jian-Qiang, HONG Hui-Long, ZHANG Yong, GU Yong-Zhe, GAO Hua-Wei, ZHOU Ya, CAO Jie, QI Hang, ZHAO Quan, BAO Li-Gao, CHEN Qing-Shan, QIU Li-Juan. Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean[J]. Acta Agronomica Sinica, 2023, 49(10): 2621-2632.

图/表 11

表1

图1

图2

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图3

表3

表4

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图5

附表1

定位区间内候选基因"

基因名称 Gene name	推测功能 Putative function
Glyma.20G114400	无注释 No annatation
Glyma.20G114500	二氢硫辛酰胺转乙酰酶 Dihydrolipoamide acetyltransferase
Glyma.20G114600	糖脂转移蛋白相关 Glycolipid transfer protein related
Glyma.20G114700	紫外线切除修复蛋白RAD23 UV excision repair protein RAD23
Glyma.20G114800	无注释 No annatation
Glyma.20G114900	无注释 No annatation
Glyma.20G114933	酸性N端SPT6 Acidic N-terminal SPT6
Glyma.20G114966	无注释 No annatation
Glyma.20G115000	PPR重复序列(PPR) PPR repeat (PPR)
Glyma.20G115100	Kow结构域转录因子1 Kow domain transcription factor 1
Glyma.20G115200	无注释 No annatation
Glyma.20G115300	DNA结合/转录因子 DNA binding/transcription factor
Glyma.20G115500	3-酮酰基辅酶a合酶5相关 3-ketoacyl-co a synthase 5
Glyma.20G115600	B-box锌指 B-box zinc finger
Glyma.20G115700	LOB结构域-包含蛋白42 LOB domain-containing protein 42
Glyma.20G115800	无注释 No annatation
Glyma.20G115900	无注释 No annatation
Glyma.20G116000	无注释 No annatation
Glyma.20G116100	50S核糖体蛋白L15, 叶绿体 50S ribosomal protein L15, chloroplast
Glyma.20G116200	锌指蛋白JAGGED相关 Zinc finger protein JAGGED related
Glyma.20G116300	蛋白质ISTR-1, 同功型A Protein ISTR-1, Isoform A
Glyma.20G116400	磷脂酶相关的//α/β-水解酶样蛋白 Phospholipase-related//α/β-hydrolase-like protein
Glyma.20G116500	腺苷酸异戊烯转移酶1, 叶绿体相关 Isopentene adenylate transferase 1, chloroplast correlation
Glyma.20G116600	VQ基序(VQ) VQ motif (VQ)
Glyma.20G116700	无注释 No annatation
Glyma.20G116800	单链核酸内切酶Single-stranded-nucleate endonuclease
Glyma.20G116900	酰转移酶 Acyltransferase
Glyma.20G117000	MYB样DNA结合蛋白MYB//MYB转录因子 MYB-like DNA binding protein MYB//MYB transcription factor
Glyma.20G117100	tRNA合成酶I类(E和Q), 催化结构域(tRNA-synt_1c) tRNA synthetases class I (E and Q), catalytic domain (tRNA-synt_1c)
Glyma.20G117150	谷氨酰胺-tRNA合成酶, 非特异性RNA结合区第1部分( tRNA_synt_1c_R1) Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1 (tRNA_synt_1c_R1)
Glyma.20G117200	无注释 No annatation
Glyma.20G117300	BAX抑制剂相关//未命名的亚家族 BAX inhibitor related//unnamed subfamily
Glyma.20G117400	无注释 No annatation
Glyma.20G117500	棉纤维表达蛋白(DUF761) Cotton fibre expressed protein (DUF761)
Glyma.20G117600	半胱氨酸-TRNA合成酶//半胱氨酸-TRNA连接酶 Cysteine-TRNA synthetase//cysteine-TRNA ligase
Glyma.20G117700	无注释 No annatation

附表1

图6

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环境 Environment	亲本Parents		重组自交系RIL
环境 Environment	齐黄34 Qihuang 34	东生16 Dongsheng 16	均值 Mean	标准差 SD	最大值 Max.	最小值 Min.	峰度 Skewness	偏度 Kurtosis	变异系数 CV (%)
2021_CP	27.97±0.20	16.59±0.16	23.97	3.69	35.79	10.65	0.00	0.41	15.40
2022_SY	32.67±0.69	27.38±1.68	29.71	3.68	40.40	19.73	0.12	-0.15	12.40
2022_CP	29.48±2.22	22.63±1.29	25.64	2.78	35.30	16.58	0.77	0.09	10.83
BLUP	29.69±1.01	22.55±0.43	26.44	2.22	33.57	20.10	0.16	0.12	8.38

连锁群 Linkage group	标记数 No. of markers	总图距 Map distance (cM)	平均图距 Average map distance (cM)	间距<5 cM Gaps<5 cM (%)	最大间距 Max. gap (cM)
Gm01	348	145.08	0.42	100.00	4.54
Gm02	372	156.70	0.42	99.46	5.56
Gm03	365	167.92	0.46	99.18	7.46
Gm04	309	140.07	0.45	99.03	7.28
Gm05	183	160.41	0.88	98.35	5.38
Gm06	211	145.65	0.69	99.05	8.32
Gm07	371	161.22	0.44	99.46	9.49
Gm08	255	125.88	0.50	100.00	4.00
Gm09	357	133.83	0.38	99.72	5.50
Gm10	455	123.76	0.27	99.56	7.94
Gm11	131	122.39	0.94	96.92	10.95
Gm12	133	128.10	0.97	97.73	14.34
Gm13	243	125.73	0.52	99.17	10.69
Gm14	224	148.46	0.67	99.55	11.70
Gm15	625	167.77	0.27	99.36	7.90
Gm16	390	192.55	0.49	99.23	7.58
Gm17	345	148.67	0.43	98.84	6.76
Gm18	283	142.60	0.51	100.00	4.43
Gm19	484	152.16	0.32	99.79	6.31
Gm20	213	156.31	0.74	99.06	5.82
总计Total	6297	2945.26	0.47	99.17	14.34

位点 QTL	环境 Environment	染色体 Chr.	标记区间 Flanking marker	遗传区间 Genetic interval (cM)	标记物理位置(a4) Physical position of markers (a4)	阈值 LOD	表型贡献率 PVE (%)	加性效应 Add	已报道的区间 Reported QTLs
qSW6-1	2021_CP	6	Marker857319-Marker851862	43.5-44.5	28822155-35028781	4.18	4.01	-0.66 a	[29], [30]
qSW6-2	2022_SY	6	Marker870421-Marker829649	90.5-95.5	10214058-12141974	6.05	5.39	-0.80 a	[31]
qSW6-2	BLUP	6	Marker870421-Marker829649	90.5-95.5	10214058-12141974	3.61	3.23	-0.37 a	[31]
qSW7-1	2022_SY	7	Marker2815425-Marker2794476	91.5-94.5	6672130-7004632	5.61	5.03	0.76 b	[32]
qSW7-2	2022_CP	7	Marker2748563-Marker2730119	87.5-88.5	7479038-7542682	10.81	9.33	0.90 b	[32]
qSW12-1	2022_CP	12	Marker5062944-Marker4965351	49.5-50.5	18776850-21043651	3.71	3.06	0.51 b	新位点New
qSW13-1	2022_SY	13	Marker3590403-Marker3588064	85.5-87.5	36310106-36606816	5.74	5.05	-0.77 a	[33]
qSW15-1	2021_CP	15	Marker4129867-Marker3915380	28.5-29.5	24348758-26925946	5.50	5.62	0.77 b	[34]
qSW15-1	BLUP	15	Marker4129867-Marker3915380	28.5-29.5	24348758-26925946	7.30	6.89	0.53 b	[34]
qSW15-2	2022_SY	15	Marker4103818-Marker3854574	25.5-26.5	43842763-44130443	6.70	5.99	0.83 b	新位点New
qSW17-1	2021_CP	17	Marker5995186-Marker5989688	80.5-86.5	7982531-8957550	3.15	3.14	-0.57 a	[35]
qSW18-1	2022_CP	18	Marker4315158-Marker4223583	65.5-68.5	12642699-14065422	5.09	4.28	-0.61 a	新位点New
qSW20-1	2021_CP	20	Marker2521089-Marker2536819	38.5-39.5	35867412-36014827	13.78	14.26	-1.23 a	[36]
	2022_SY	20	Marker2377057-Marker2521089	36.5-38.5	35579408-36014827	18.06	18.10	-1.45 a	[36]
	2022_CP	20	Marker2377057-Marker2521089	35.5-38.5	35579408-36014827	10.85	9.73	-0.92 a	[36]
	BLUP	20	Marker2416012-Marker2448322	39.5-40.5	35916231-35920512	19.87	19.04	-0.89 a	[36]

大豆百粒重稳定QTL qSW20-1定位及对产量和品质的影响

Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean

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