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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 173-178.doi: 10.3724/SP.J.1006.2009.00173

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Analvsis of Sichuan Sun-Cured Tobacco Germplasm by SRAP

LONG Teng1,LIU Lei2,HUANG Yu-Bi1.2.*   

  1. 1Maize Research Institute of Sichuan Agricultural University;2Agronomy College of sichuan Agricultural University,Ya'an 625014,China
  • Received:2008-03-28 Revised:2008-07-13 Online:2009-01-12 Published:2008-11-18
  • Contact: HUANG Yu-Bi

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Abstract:

SRAP was used to analyze genetic relationships of 25 sun-cured tobacco varieties, one Nicotiana rustica variety, and one flue-cured tobacco variety collected from Sichuan province. PCR products were detected by polyacrylamide gel electrophoresis (PAGE). A total of 3 368 DNA fragments were obtained, of which 998 were specific bands (polymorphic ratio was 29.6%), the average number of amplified DNA bans was 40 per primer．Genetic similarity ranged from 0.26 to 0.97 for all varieties, from 0.38 to 0.78 between Nicotiana rustica varieties, flue-cured tobacco varieties and sun-cured tobacco varieties, and from 0.75 to 0.97 among 25 Sun-cured tobacco varieties. Cluster analysis was carried out with unweighted pair group method with arithmetic mean (UPGMA)．The results showed that all 27 varieties could be clustered into three groups, which revealed the genetic diversity among three cultivar-type tobacco germplasms. Furthermore, 25 sun-cured tobacco varieties could be classified into three subgroups and two single varieties. Maoyan and Liuyan were two traditional famous groups of Sichuan sun-cured tobacco species. However, Maoyan and Liuyan varieties named by traditional method were not obviously grouped into two types. All results indicated that SRAP analysis may be used to reveal the genetic relationships among sun-cured tobacco varieties, providing a scientific basis for tobacco genetic research and breeding.

Key words: Sun-cured tobacco, Germplasm, Genetic relationship, SARP

[1]Jia J-Z(贾继增). Molecular germplasm diagnostics and molecular marker-assisted breeding. Sci Agric Sin (中国农业科学), 1996, 29(4): 1–10 (in Chinese with English abstract)
[2]Li G, Quiros C F. Sequence related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455–461
[3]Li G, Gao M, Yang B, Quiros C F. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transeriptome mapping. Theor Appl Genet, 2003, 107: 168–180
[4]Jiang S-K(姜树坤), Zhang X-J(张喜娟), Zhang L(张丽). Blast resistance gene mapping and linked SRAP fragment analysis of rice (Oryza sativa L.) ‘Shennong 606’. Plant Physiol Commun (植物生理学通讯), 2007, 43(5): 852–856(in Chinese with English abstract)
[5]Wen Y-C(文雁成), Wang H-Z(王汉中), Shen J-X(沈金雄), Liu G-H(刘贵华), Zhang S-F(张书芬). Analysis of genetic diversity and genetic basis of Chinese rapeseed cultivars (Brassica napus L.) by sequence-related amplified polymorphism markers. Sci Agric Sin (中国农业科学), 2006, 39(2): 246–256(in Chinese with English abstract)
[6]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春). Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton. Acta Genet Sin (遗传学报), 2004, 31(6): 622–626(in Chinese with English abstract)
[7]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春), He D-H(贺道华), Wu M-Q(吴茂清). Construction of SRAP linkage map in cotton. Chin Sci Bull (科学通报), 2003, 48: 1676–1679(in Chinese with English abstract)
[8]Wang H-Z(王华忠), Wu Z-D(吴则东), Wang X-W(王晓武), Fang Z-Y(方智远). Analysis of the genetic diversity in different types of sugar beets by SRAP and SSR markers. Acta Agron Sin (作物学报), 2008, 34(1): 37–46(in Chinese with English abstract)
[9]Dudak H, Shearman R C, Parmaksiz I. Molecular characterization of Buffalo grass germplasm using sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328–334
[10]He F-F(何凤发), Yang Z-P(杨志平), Zhang Z-S(张正圣), Wang G-X(王贵学), Wang J-C(王季春). Genetic diversity analysis of potato germplasm by SRAP makers. J Agric Biotechnol (农业生物技术学报), 2007, 15(6): 1001–1005(in Chinese with English abstract)
[11]Wang Z-D(王志德), Mou J-M(牟建民), Dai P-G(戴培刚), Wang W-F(王伟峰), Jiang Y-E(蒋予恩). Some tobacco core plant the quality RAPD analysis. Acta Tab Sin (中国烟草学报), 2003, 9(4): 20–26(in Chinese with English abstract)
[12]Liang J-X(梁景霞), Qi J-M(祁建民), Fang P-P(方平平), Wang T(王涛), Chen S-H(陈顺辉), Zhou D-X(周东新), Tao A-F(陶爱芬), Liang K-J(梁康迳), Wu W-R(吴为人). Genetic diversity and genetic relatives analysis of tobacco germplasm based on inter-simple sequence repeat (ISSR). Sci Agric Sin (中国农业科学), 2008, 41(1): 286–294 (in Chinese with English abstract)
[13]Yang B-C(杨本超), Xiao B-G(肖炳光), Chen X-J(陈学军), Shi C-H(石春海). Genetic diversity of flue-cured tobacco varieties based on ISSR makers. Hereditas (遗传), 2005, 27(5): 753–758(in Chinese with English abstract)
[14]Qi J-M(祁建民), Wang T(王涛), Chen S-H(陈顺辉), Zhou D-X(周东新), Fang P-P(方平平), Tao A-F(陶爱芬), Liang J-X(梁景霞), Wu W-R(吴为人). Genetic diversity and genetic relatives analysis of tobacco germplasm based on inter-simple sequence repeat (ISSR). Sci Agric Sin (中国农业科学), 2006, 32(3): 373–378(in Chinese with English abstract)
[15]Ge Y-Q(葛永奇), Qiu Y-X(邱英雄), Ding B-Y(丁炳扬), Fu C-X(傅承新). An ISSR analysis on population genetic diversity of the relict plant Ginkgo biloba. Biodiver Sci (生物多样性), 2003, 11(4): 276–287(in Chinese with English abstract)
[16]Du C-Y(杜传印), Liu H-X(刘洪祥), Tian J-C(田纪春). Phylogenetic analysis of partial tobacco germplasm resources with AFLP markers. Acta Agron Sin (作物学报), 2006, 32(10): 1592–1596(in Chinese with English abstract)
[17]Ren N, Timko M P. AFLP analysis of genetic polymorphism and evolutionary relationships among cultivated and Nicotiana species. Genome, 2001, 44: 559–571
[18]Liang J-X(梁景霞), Qi J-M(祁建民), Wu W-R(吴为人), Zhou D-X(周东新), Chen S-H(陈顺辉), Wang T(王涛), Tao A-F(陶爱芬). DNA extraction and the establishment of SRAP reaction system in tobacco. Acta Tab Sin (中国烟草学报), 2005, 11(4): 33–38(in Chinese with English abstract)
[19]Nei M, Li W H. Mathematical model for studying genetic variation interms of restriction endonueleases. Proc Natl Acad Sci USA, 1979, 76: 5269–5273
[20]Ferriol M, Pico B, Cordava P F. Molecular diversity of a germplasm collection of sqash (Cucurbita moschata) determined by SRAP and AFLP makers. Crop Sci, 2004, 44: 653–664
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