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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 418-423.doi: 10.3724/SP.J.1006.2009.00418

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Proteome Analysis on Resistance to Phytophora Root Rot in Soybean

QIU Hong-Mei13;LIU Chun-Yan12**;ZHANG Dai-Jun3;XIN Xiu-Jun3;WANG Jia-Lin1;WANG Jing1;SHAN Da-Peng1;HU Gu-Hua2*;CHEN Qing-Shan1*   

  1. 1Soybean Research Institute, Northeast Agricultural University, Harbin 150030,China;2Land Reclamation Research & Breeding Centre of  Heilongjiang,Harbin 150090,China;3Hongxinglong Research Institute of Heilongjiang Land Reclamation Bureau,Youyi 155811,China
  • Received:2008-06-17 Revised:2008-10-22 Online:2009-03-12 Published:2009-01-15
  • Contact: CHEN Qing-Shan

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Abstract:

Phytophthora root rot caused by Phytophthora sojae is a serious soil-borne fungi disease endangeringsoybean production and bringing huge economic losses. The objective of this study was to survey and identify the proteins associated with resistance, using soybean cultivar Suinong 10 by two-dimensional gel electrophoresis and mass spectrometry technology.Nineteen differentially expressed proteins were obtained, of twelve which presented up-regulated, six were down-regulated, and one was special protein. Ten protein spots including eight with up-regulated expression, one with down-regulated expression, and one with specially expression were investigated by biological mass spectrometry. The results showed that eight functional proteins were identified, three of which involved inmetabolism, consisting of the ribolose-1,5-phosphate carboxylase large subunit and its precursor, succinyl coenzyme A, three of which were related to signal transduction, encompassing kinase receptor like protein, oxidoreductase, and cysteamine ammonia acid oxidoreductase, one of which participated in transport of metabolites in cells, i.e. clothing zeta-3 protein subunits, one of which was concerned with the jasmonic acid-mediated resistance, including F-box protein. The results provide useful information for studying the molecular mechanism of resistance to Phytophthora root rot.

Key words: Soybean, Phytophoya root rot , Resistance-related protein, 2D, MALDI-TOF-MS

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