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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 424-431.doi: 10.3724/SP.J.1006.2009.00424

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Behaviour of Thinopyrum intermedium Chromosome 2Ai-2 in Different  Wheat Chromosome Substitution Backgrounds of Group 2

ZHANG Yue12;LIN Zhi-Shan1*;CAO Bao-Jiu3;GUO Yi-Qiang3;WANG Mei-Jiao14;YE Xing-Guo1;XIN Zhi-Yong1;XU Qiong-Fang1;GUO Shi-Hua2   

  1. 1National Key Facility for Crop Genetic Resources and Genetic Improvement/Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081,China;2 Agricultural College, Inner Mongolia Agricultural University,Hohhot 010018,China;3College of Life Sciences and Bioengineering, North University for Nationalities, Yinchuan 750021,China,4College of  Plant Science,Jilin University, Changchun 130062,China
  • Received:2008-06-30 Revised:2008-09-03 Online:2009-03-12 Published:2009-01-15

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Abstract:

Thinopyron intermedium is one of the important gene sources for high resistances to barley yellow dwarf virus, stripe rust as well as tolerances to cold and drought in wheat (Triticum aestivum L.) breeding. Several wheat–Th. intermedium addition lines, substitution lines, and translocation lines have been developed as breeding materials, including the 2Ai-2 substitution lines with high resistances to the GPV and GAV strains of barley yellow dwarf virus. The aim of this study was to provide cytogenetical evidence on the behavour of 2Ai-2 chromosome of Th. intermedium in different wheat chromosome substitution backgrounds. Five wheat–alien disomic (or ditelosomic) substitution lines were crossed with common wheat variety Chinese Spring to generate the BC1 and F2 populations, and the two populations were detected using EST-PCR markers specific to 2Ai-2 chromosome and genomic in situ hybridization (GISH). In the F2 generation, plants with 2Ai-2 accounted for 83.1% and 82.4% in offsprings of the 2Ai-2 (2B) substitution lines N420 and N439, respectively, which were higher thantheexpected ratio of 75%; whereas, the observed ratios in offsprings of the 2Ai-2 (2D) substitution lines N431 and N452 were 67.6% and 53.8%, respectively, which were lower than the expected value. Especially, the observed value in the hybrids of N452 was significantly different from the expected value (P < 0.01), inferring that the alien chromosome or fragment might be preferentially transmitted into F2 generation in 2Ai-2 (2B) substitution lines through the corresponding gametes. On the contrary, the transmission ratio of the alien chromosome or fragment was low in 2D-subsititution background. It was concluded that the effect of different substitution background on the transfer of the 2Ai-2 chromosome was different. In addition, in both 2B and 2D subsititution backgrounds, the transmission ratio of the alien chromosome or fragment was obviously higher through male gametes than female gametes. Furthermore, many plants with 2Ai-2 in F2 and BC1 were detected using 2Ai-2-specific EST-PCR markers in a limited region, but not in other region, indicating that the alien chromosome was unstable when it was transmitting from hybrid to subsequent generations. From the results, it was inferred that most chromosome variances occurred near centromere. Compared with the short arm, the long arm of the alien chromosome was lost more often in the next generations. The F2 plants with 2AS-substitution background (N530) were very few when detected with 2Ai-2S-specific EST-PCR marker(s).The difference between observed and expected values was significant (P < 0.01), suggesting that the alien telosome might be lost freguently. Structure variationoftelosome 2Ai-2S was also observed inthe offsprings with2AS-substitution background. GISH results confirmed that the EST-PCR markers can be used effectively in tracing the alien chromosome in wheat background.

Key words: Disomic substitution lines, EST-PCR markers, Chromosome, Transmission ratio, Structure variation

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