Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 432-437.doi: 10.3724/SP.J.1006.2009.00432

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of a Novel Anthracnose Resistance Gene Using SSR Markers in Common Bean(Phaseolus vulgaris L.)

WANG Kun;WANG Xiao-Ming;ZHU Zhen-Dong;ZHAO Xiao-Yan;WANG Shu-Min   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081,China
  • Received:2008-08-29 Revised:2008-12-07 Online:2009-03-12 Published:2009-01-16
  • Contact: WANG Shi-Min

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Abstract:

Anthracnose (Colletotrichum lindemuthianum) is one of the most important diseases of common bean throughout the world. Resistant cultivars utilization is the most economic, effective and environment harmless method to control this disease. However, disease resistance in the host is unsustainable due to the high variability of anthracnose. So it is necessary to explore the new resistance genes persistently. Among the 11 reported resistant loci, 10 of them originated from Mesoamerican gene pool and only one locus named Co-1 from Andean gene pool. The objective of this study was to identify the new Andean gene resistant to anthracnose in the Chinese common bean landraces. 108 F2 plants derived from a cross between common bean Red flower (R) and common bean Jing (S) were inoculated with anthracnose race 81. The resistant individuals grew well without obvious disease symptoms but the susceptible plants have many disease spots on the leaves and stems, and then died gradually. The segregation ratio of resistant to susceptible plants in F2 population was 3:1 based on chi-square test, indicating that the resistance of Red flowercommon bean to anthracnose race 81 was controlled by a single dominant gene, named as Co-F2533 temporarily. Four SSR markers were detected on B6 linkage group of common bean that linked to the resistance gene Co-F2533 with the distance of 6.6, 18.4, 20.9, and 30.9 cM respectively. The position of the resistance gene and the markers on the chromosome were as follows: Clon1429, Co-F2533, BM170, BMD37, and Clon410. A linkage map was constructedwith Mapdraw V2.1. Since the absent of anthracnose resistance genes on B6 linkage group, it is proposed that Co-F2533 is a novel anthracnose resistance gene in Chinese resistant landrace Red flower from Andean gene pool, which will be useful in the anthracnose resistance breeding for common bean.

Key words:   Common bean, Anthracnose, Resistant gene, SSR marker

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