Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 432-437.doi: 10.3724/SP.J.1006.2009.00432

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of a Novel Anthracnose Resistance Gene Using SSR Markers in Common Bean(Phaseolus vulgaris L.)

WANG Kun;WANG Xiao-Ming;ZHU Zhen-Dong;ZHAO Xiao-Yan;WANG Shu-Min   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081,China
  • Received:2008-08-29 Revised:2008-12-07 Online:2009-03-12 Published:2009-01-16
  • Contact: WANG Shi-Min

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Abstract:

Anthracnose (Colletotrichum lindemuthianum) is one of the most important diseases of common bean throughout the world. Resistant cultivars utilization is the most economic, effective and environment harmless method to control this disease. However, disease resistance in the host is unsustainable due to the high variability of anthracnose. So it is necessary to explore the new resistance genes persistently. Among the 11 reported resistant loci, 10 of them originated from Mesoamerican gene pool and only one locus named Co-1 from Andean gene pool. The objective of this study was to identify the new Andean gene resistant to anthracnose in the Chinese common bean landraces. 108 F2 plants derived from a cross between common bean Red flower (R) and common bean Jing (S) were inoculated with anthracnose race 81. The resistant individuals grew well without obvious disease symptoms but the susceptible plants have many disease spots on the leaves and stems, and then died gradually. The segregation ratio of resistant to susceptible plants in F2 population was 3:1 based on chi-square test, indicating that the resistance of Red flowercommon bean to anthracnose race 81 was controlled by a single dominant gene, named as Co-F2533 temporarily. Four SSR markers were detected on B6 linkage group of common bean that linked to the resistance gene Co-F2533 with the distance of 6.6, 18.4, 20.9, and 30.9 cM respectively. The position of the resistance gene and the markers on the chromosome were as follows: Clon1429, Co-F2533, BM170, BMD37, and Clon410. A linkage map was constructedwith Mapdraw V2.1. Since the absent of anthracnose resistance genes on B6 linkage group, it is proposed that Co-F2533 is a novel anthracnose resistance gene in Chinese resistant landrace Red flower from Andean gene pool, which will be useful in the anthracnose resistance breeding for common bean.

Key words:   Common bean, Anthracnose, Resistant gene, SSR marker

[1] McClean P, Kami J, Gepts P. Genomics and genetic diversity in common bean. In: Wilson R F, Stalker T H, Brummer E C eds. Legume Crop Genomics. USA: AOCS Publishing, 2004. pp 61–82
[2] Zhong X-X(宗绪晓). High-Yield Cultivation and Consumption of Legume Food Processing (食用豆类高产栽培与食品加工). Beijing: China Agricultural Science and Technology Press, 2002. pp 227–246(in Chinese)
[3] Pastor-Corrales M A. Anthracnose. In: Pastor-Corrales M A, Tu J C, eds. Bean Production Problems in the Tropics. Centro Internacional de Agricultura Tropical, Cali, Colombia, 1989. pp 77–104
[4] Wang X-M(王晓鸣), Li Y-L(李怡琳), Li S-Y(李淑英). Study of identification the resistance to anthracnose of common bean germplasm. Crop Germplasm Resours (作物品种资源), 1989, (2): 18–19(in Chinese)
[5] Kelly J D, Vallejo V A. A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. Hort Sci, 2004, 39: 1196–1207
[6] Gepts P. A middle American and an Andean common bean gene pool. In: Gepts P ed. Genetic Resources of Phaseolus Beans. London: KIuwer, 1988. pp 375–390
[7] Kelly J D. Use of randomly amplified polymorphic DNA markers in breeding for major gene resistance to plant pathogens. J Am Soc Hort Sci, 1995, 30: 461–465
[8] Young R A, Kelly J D. Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. Plant Dis, 1996, 80: 650–654
[9] Wu Q-A(吴全安). Identification of crops germplasm resources resistant to diseases and insect pests. Beijing: Agriculture Press, 1991. pp 60–61(in Chinese)
[10] Afanador L, Hadley S, Kelly J D. Adoption of a mini-prep DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L). Annu Rept Bean Improv Coop, 1993, 36: 10–11
[11] Michelmore R M, Paran I, Kesseli R V. Identification of marker linked to disease- resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[12] Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: An interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics, 1987, 1: 174–181
[13] Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172–175
[14] Liu R-H(刘仁虎), Meng J-L(孟金陵). MapDraw: A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (遗传), 2003, 25(3): 317–21(in Chinese with an English abstract)
[15] Wang X-M(王晓鸣), Li Y-L(李怡琳), Luo P-X(骆平西), Zhao-Y-L(赵玉玲). The resistance of Chinese dry bean germplasm. Crop Germplasm Resourse (作物品种资源), 1999, (1): 25–28(in Chinese)
[16] Xu Z-S(徐兆生), Wang M(王幕), Wei M(魏民), Yu H-J(余宏军). Inheritance of resistance to anthracnose in snapbean. China Vegetables (中国蔬菜), 1999, (4): 18–19(in Chinese)
[17] Zhao X-Y(赵晓彦). Identification of anthracnose resistant genes based on molecular markers and genetic diversity analysis of resistant germplasm in common bean. MS Dissertation of Chinese Academy of Agricultural Science, 2006(in Chinese with English abstract)
[18] Duran L A, Blair M W, Giraldo M C, Machiavelli R, Prophete E, Nin J C and Beaver J S. Morphological and molecular characterization of common bean (Phaseolus vulgaris L) landraces from the Caribbean. Crop Sci, 2005, 45: 1320–1328
[19] Evans A M. Structure, variation, evolution, and classification in Phaseolus. In: Summer R J, Bunting A H, eds. Advances in Legume Science. Royal Botanic Gardens, Kew, UK, 1980. pp 337–347
[20] Wang X-M(王晓鸣), Li Y-L(李怡琳), Wu X-F(武小菲). Pathogenic variant of Chinese Colletotrichum lindemuthianum. J Shandong Agric Univ (山东农业大学学报), 1999, 30(suppl): 125–129(in Chinese)
[21] Wang K(王坤), Wang X-M(王晓鸣), Zhu Z-D(朱振东), Zhang X-Y(张晓艳), Wang S-M(王述民). Identification of Colletotrichum lindemuthianum races and bean germplasm evaluation for anthracnose resistance in eight provinces of China. J Plant Genet Resour (植物遗传资源学报), 2008, 9(2): 168–172(in Chinese with English abstract)
[22] Melotto M, Coelho M F, Pedrosa-Harand A, Kelly J D, Camargo L E A. The anthracnose resistance locus Co-4 of common bean is located on chromosome 3 and contains putative disease resistance related genes. Thero Appl Genet, 2004, 109: 122–134
[23] Mendoza A, Hernández F, Hernández S, Ruíz D, Martínez de la Vega O, Mora G, Acosta J, Simpson J. Identification of Co-1 anthracnose resistance and linked molecular markers in common bean line A193. Plant Dis, 2001, 85: 252–255
[24] Young R A, Kelly J D. Tests of allelism for the anthracnose resistance Co-1 gene. Annu Rept Bean Improv Coop, 1997, 40: 128–129
[25] Yu K F, Park S J, Poysa V. Abundance and variation of microsatellite DNA sequences in beans (Phaseolus vulgaris L). Genome, 1999, 42: 27–34
[26] Yu K F, Park S J, Poysa V, Gepts P. Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). Heredity, 2000, 91: 429–434
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