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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 424-431.doi: 10.3724/SP.J.1006.2009.00424

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Behaviour of Thinopyrum intermedium Chromosome 2Ai-2 in Different  Wheat Chromosome Substitution Backgrounds of Group 2

ZHANG Yue12;LIN Zhi-Shan1*;CAO Bao-Jiu3;GUO Yi-Qiang3;WANG Mei-Jiao14;YE Xing-Guo1;XIN Zhi-Yong1;XU Qiong-Fang1;GUO Shi-Hua2   

  1. 1National Key Facility for Crop Genetic Resources and Genetic Improvement/Key Laboratory of Crop Genetics and Breeding,Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081,China;2 Agricultural College, Inner Mongolia Agricultural University,Hohhot 010018,China;3College of Life Sciences and Bioengineering, North University for Nationalities, Yinchuan 750021,China,4College of  Plant Science,Jilin University, Changchun 130062,China
  • Received:2008-06-30 Revised:2008-09-03 Online:2009-03-12 Published:2009-01-15

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Abstract:

Thinopyron intermedium is one of the important gene sources for high resistances to barley yellow dwarf virus, stripe rust as well as tolerances to cold and drought in wheat (Triticum aestivum L.) breeding. Several wheat–Th. intermedium addition lines, substitution lines, and translocation lines have been developed as breeding materials, including the 2Ai-2 substitution lines with high resistances to the GPV and GAV strains of barley yellow dwarf virus. The aim of this study was to provide cytogenetical evidence on the behavour of 2Ai-2 chromosome of Th. intermedium in different wheat chromosome substitution backgrounds. Five wheat–alien disomic (or ditelosomic) substitution lines were crossed with common wheat variety Chinese Spring to generate the BC1 and F2 populations, and the two populations were detected using EST-PCR markers specific to 2Ai-2 chromosome and genomic in situ hybridization (GISH). In the F2 generation, plants with 2Ai-2 accounted for 83.1% and 82.4% in offsprings of the 2Ai-2 (2B) substitution lines N420 and N439, respectively, which were higher thantheexpected ratio of 75%; whereas, the observed ratios in offsprings of the 2Ai-2 (2D) substitution lines N431 and N452 were 67.6% and 53.8%, respectively, which were lower than the expected value. Especially, the observed value in the hybrids of N452 was significantly different from the expected value (P < 0.01), inferring that the alien chromosome or fragment might be preferentially transmitted into F2 generation in 2Ai-2 (2B) substitution lines through the corresponding gametes. On the contrary, the transmission ratio of the alien chromosome or fragment was low in 2D-subsititution background. It was concluded that the effect of different substitution background on the transfer of the 2Ai-2 chromosome was different. In addition, in both 2B and 2D subsititution backgrounds, the transmission ratio of the alien chromosome or fragment was obviously higher through male gametes than female gametes. Furthermore, many plants with 2Ai-2 in F2 and BC1 were detected using 2Ai-2-specific EST-PCR markers in a limited region, but not in other region, indicating that the alien chromosome was unstable when it was transmitting from hybrid to subsequent generations. From the results, it was inferred that most chromosome variances occurred near centromere. Compared with the short arm, the long arm of the alien chromosome was lost more often in the next generations. The F2 plants with 2AS-substitution background (N530) were very few when detected with 2Ai-2S-specific EST-PCR marker(s).The difference between observed and expected values was significant (P < 0.01), suggesting that the alien telosome might be lost freguently. Structure variationoftelosome 2Ai-2S was also observed inthe offsprings with2AS-substitution background. GISH results confirmed that the EST-PCR markers can be used effectively in tracing the alien chromosome in wheat background.

Key words: Disomic substitution lines, EST-PCR markers, Chromosome, Transmission ratio, Structure variation

[1] Liu S-X(刘四新), Xin Z-Y(辛志勇). The development of wheat-rye addition lines resistant to powdery mildew. Acta Agron Sin (作物学报), 1993, 19(5): 395–403 (in Chinese with English abstract)
[2] Zhang X-Y(张学勇), Li Z-S(李振声), Chen S-Y(陈漱阳). Studies on the nullisomic backcrossing procedures for producing alien substitution lines of common wheat. Acta Genet Sin (遗传学报), 1989, 16(6): 420–431 (in Chinese with English abstract)
[3] Sears E R. Relationships of chromosomes 2A, 2B and 2D with their rye homoeologue. In: Finalay K W, Shepherd K W eds. Proc. 3rd Intl. Wheat Genet Symp. Canberra, Austrilia, 1968. pp 53–61
[4] Zhang X-Y(张学勇), Dong Y-C(董玉琛), Yang X-M(杨欣明). Cytogenetic research on hybrids of Triticum with both Thinopyrum proticum and Th. intermedium as well as their derivatives. Acta Genet Sin (遗传学报), 1995, 22(3): 217–222 (in Chinese with English abstract)
[5] Zhang W-J(张文俊), Jing J-K(景健康), Hu H(胡含). The transmission of rye chromosome 6R in wheat background. Acta Genet Sin (遗传学报), 1995, 22(3): 211–216 (in Chinese with English abstract)
[6] Tang S-X(唐顺学), Liang H(梁辉), Li Y-W(李义文), Jia S-E(贾双娥), Li Z-S(李振声), Jia X(贾旭). The genetic behavior of an Agropyron intermedium chromosome conferring BYDV resistance in wheat background. Acta Genet Sin (遗传学报), 2000, 27(6): 520–526 (in Chinese with English abstract)
[7] Wang H-G(王洪刚), Liu S-B(刘树兵), Qi Z-J(亓增军), Kong F-J(孔凡晶), Gao J-R(高居荣). Application studies of Elytrigia intermedium in hereditary improvement of wheat. J Shandong Agric Univ (山东农业大学学报), 2000, 31(3): 333–336 (in Chinese)
[8] Wang H-G(王洪刚), Liu S-B(刘树兵), Li X-F(李兴锋), Gao J-R(高居荣), Feng D-S(封德顺), Chen D-H(陈冬花). Breeding and identification of six octoploid trititrigia. J Triticeae Crops (麦类作物学报), 2006, 26(4): 6–10 (in Chinese with English abstract)
[9] Zhang Z-Y(张增艳), Xin Z-Y(辛志勇). Advances in barley yellow dwarf virus resistance heredity in wheat breeding. Crops (作物杂志), 1998, (4): 6–7 (in Chinese)
[10] Cauderon Y, Saigne B, Dange M. The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. Proc 4th Intl wheat Genet Symp. Missouri: University of Missouri, 1973. pp 401–407
[11] Larkin P J, Banks P M, Lagudah E S, Appels R, Chen X, Xin Z Y, Ohm H W, McIntosh R A. Disomic Thinopyrum intermedium addition lines in wheat with barley yellow dwarf virus resistance and with rust resistance. Genome, 1995, 38: 385–394
[12] Lin Z-S, Huang D-H, Du L-P, Ye X-G, Xin Z-Y. Identification of wheat–Thinopyrum intermedium 2Ai-2 ditelosomic addition and substitution lines with resistance to barley yellow dwarf virus. Plant Breed, 2006, 125: 114–119
[13] Ai S-J(艾山江·阿布都拉), Wen Y-X(文玉香), Tang S-X(唐顺学), Li Y-W(李义文), Zhuang J-J(庄家骏), Jia X(贾旭). Breeding and cytological and molecular identification of a new germplasm with multiple disease resistance from Trititum aestivum–Agropyon intermedium. Acta Genet Sin (遗传学报), 1997, 24(5): 441–446 (in Chinese with English abstract)
[14] Lin Z S, Cui Z F, Zeng X Y, Ma Y Z, Zhang Z Y, Nakamura T, Ishikawa G, Nakamura K, Yoshida H, Xin Z Y. Analysis of wheat-Thinopyrum intermedium derivatives with BYDV-resistance. Eupytica, 2007, 158: 109–118
[15] Xin Z-Y(辛志勇), Xu H-J(徐惠君), Chen X(陈孝), Lin Z-S(林志珊). Introduction of genes resistant to barley yellow dwarf virus into common wheat by biotechnology. Sci China (Ser B) (中国科学·B辑), 1991, 21(1): 36–36 (in Chinese)
[16] Xu H-J(徐惠君), Xin Z-Y(辛志勇), Liu S-X(刘四新), Chen X(陈孝), Du L-P(杜丽璞). Development of common wheat alien translocation lines by tissue culture. Acta Genet Sin (遗传学报), 1996, 23(5): 376–381 (in Chinese with English abstract)
[17] Zhang Z-Y(张增艳), Xin Z-Y(辛志勇), Ma Y-Z(马有志), Chen X(陈孝), Xu Q-F(徐琼芳), Lin Z-S(林志珊). Mapping of a BYDV resistance gene from Th. intermedium in wheat background by molecular markers. Sci China (Ser C) (中国科学·C辑), 1999, 42(6): 663–668 (in Chinese with English abstract)
[18] Bassam B J, Caetano-Anolles G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gel. Anal Biochem, 1991, 196: 80–83
[19] Wei W H, Qin R, Song Y C, Ning S B, Guo L Q, Gu M G. Location and analysis of introgressed segments in the parthenogenetic progenies of Zea mays × Z. diploperennis by GISH. Acta Bot Sin, 2002, 44: 373–376
[20] Yuan Y, Chen X, Xiao S, Islam A K M R. The improving wheat starch quality by transferring barley a-amylase inhibitor gene to wheat. In: Proc10th Intl Wheat Genetics Symp, Paestum, Italy, 2003. pp 463–466
[21] Tang S-X, Li Z-S, Jia X. Genomic in situ hybridization (GISH) analyses of Thinopyrum intermedium, its partial amphiploid Zhong 5, and disease-resistant derivatives in wheat. Theor Appl Genet, 2000, 100: 344–352
[22] Ren Z-L(任正隆). The MADI process for genetic transfer. J Sichuan Agric Univ (四川农业大学学报), 1990, 8(1): 1–6 (in Chinese with English abstract)
[23] Sears E R. Chromosome engineering in wheat. Proc Stadler Genet Symp, 1972, 4: 23–38
[24] Cui Z-F(崔志富), Lin Z-S(林志珊), Xin Z-Y(辛志勇), Tang Y-M(唐益苗), Zhang Z-Y(张增艳), Lu Q(卢勤). Identification of wheat–Thinopyrum intermedium telosomic lines resistant to barley yellow dwarf virus by GISH and STS markers converted from RFLP. Acta Agron Sin (作物学报), 2006, 32(12): 1855–1859 (in Chinese with English abstract)
[25] Li G-P(李桂萍), Chen P-D(陈佩度), Zhang R-Q(张瑞奇), Wang C-M(王春梅), Cao A-Z(曹爱忠), Zhang S-Z(张守忠). Transmission of 6VS/6AL chromosome through gametes and its genetic stability in different genetic backgrounds. J Triticeace Crops (麦类作物学报), 2007, 27(2): 183–187(in Chinese with English abstract)
[26] Ma J-X(马渐新), Zhou R-H (周荣华), Jia J-Z(贾继增), Dong Y-C(董玉琛). Genetic stability and transmission of chromosome 6V from H. villosa through gametes in wheat background. Acta Genet Sin (遗传学报), 1999, 6(4): 384–390 (in Chinese with English abstract)
[27] Tao Y-Z(陶跃之), Hu H(胡含). Transmission of rye chromosome in pollen plants derived from the hybrid 6x Triticale ′ common wheat. Acta Genet Sin (遗传学报), 1990, 17(2): 98-102 (in Chinese with English abstract)
[28] Ren Z-L(任正隆), Lelley T, Roebbelen G. Transmission of the wheat and rye chromosomes in octoploid triticale ′ common wheat population. Acta Genet Sin (遗传学报), 1991, 18(2): 161–167 (in Chinese with English abstract)
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