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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (3): 541-545.doi: 10.3724/SP.J.1006.2009.00541

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Cloning and characterization of the NAC-Like Gene AhNAC2 and AhNAC3 in Peanut

LIU Xu;LI Ling*   

  1. Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631
  • Received:2008-08-20 Revised:2008-10-06 Online:2009-03-12 Published:2009-01-16
  • Contact: LI Ling

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Abstract:

NAC transcription factors existed differentially in plant are the new transcription regulatory factors with multiple biological functions. Two NAC-like genes from peanut were cloned by RT-PCR and RACE methods, named AhNAC2 and AhNAC3 (GenBank accession No. EU755023 and EU755022), which contain an ORF of 1 050 bp and 1 008 bp and encode 349 and 335 amino acids, respectively. Gene sequence analysis showed that the putative protein of both two genes contained conserved NAC domain and highly different C terminal which is the typical characteristics of NAC transcription factors. Meanwhile, the transcription levels of the two genes were investigated by semi-quantitative RT-PCR, and the result showed that the expressions of AhNAC2 and AhNAC3 genes were enhanced by ABA, GA3, water stress, and cold stress respectively. Furthermore, the two genes expressed constitutively in peanut tissues, and their expression patterns were different in various tissues. In conclusion, AhNAC2 and AhNAC3 genes isolated from peanut were new members of NAC transcription factor family, their comparison to RD26 (AT4G27410) reveals a high amino acid homology. We suggested that they play key roles in ABA signal transduction and drought response in peanut.

Key words: NAC transcription factor, Gene expression and regulation, Signal transduction

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