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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1474-1482.doi: 10.3724/SP.J.1006.2009.01474


Comparison of Genetic Diversity between In-situ Conserved and Non-conserved Oryza rufipogon Populations in China

WANG Jia-Xiang,CHEN You-Tao,HUANG Juan,QIAO Wei-Hua,ZHANG Wan-Xia,YANG Qing-Wen*   

  1. Institute of Crop Sciences/National key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2009-02-23 Revised:2009-04-21 Online:2009-08-12 Published:2009-06-10
  • Contact: YANG Qing-Wen, E-mail: qwyang@mail.caas.net.cn; Tel: 010-62122851


By the end of 2007, altogether 15 Oryza rufipogon in-situ conservation sites had been established in different parts of China. However, these sites were selected only on the basis of the scientists’ experience and the materials submitted by different provinces. Thus, the validity of the selected populations still needs to be verified. By virtue of 24 pairs of SSR markers, the research was conducted on genetic diversity for 427 accessions of Oryza rufipogon from the 15 in-situ conserved populations and 357 accessions from 15 non-conserved populations which were randomly selected according to their latitude, so as to clarify the genetic diversity and representativeness of the existing Oryza rufipogon in situ conserved populations in China. The results indicated that the average Ae and I of the 24 lociof the conserved populations were 5.98 and 1.90 respectively, which were both higher than those of non-conserved populations (5.85 and 1.86 respectively). However, the average He of the 24 loci of the conserved populations was 0.79, which was lower than that of the non-conserved populations (0.80). Besides, test of significance on the Ae, I, and He of the SSRloci showed that there was no significant difference between the two populations, demonstrating that the genetic diversity of the conserved populations could be representative of the Oryza rufipogon populations in China. Moreover, the number of specificalleles of the conserved populations was 40, which far exceeded that of the non-conserved populations(20), showing that the conserved populations possessed more unique genes which are worth conserved. In addition, based on previous research findings and in accordance with the geographic information of the conserved populations, we found that the 15 conservation sites covered all the typical geographic types in the Oryza rufipogon distribution areas in China. Thus, we may conclude that the 15 in-situ conservation sites were scientifically and rationally selected. On the basis of the analysis of the genetic structure of the Oryza rufipogon populations in China, it was recommended that future in-situ conservation sites should be established mainly in the southern part of Guangxi and in Guangdong.

Key words: Oryza rufipogon Griff., In-situ conservation, Genetic diversity, SSR

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