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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1468-1473.doi: 10.3724/SP.J.1006.2009.01468

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Expression of Critical Lignin Metabolism Genes in Flax(Linum usitatissimum)

WANG Jin1,CHEN Xin-Bo1,2,*,GAO Yuan2,ZHANG Yan-Hong1,LONG Song-Hua1,DENG Xin1,HE Dong-Feng2,WANG Yu-Fu1   

  1. 1Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences/Key Laboratory of Genetic Improvement & Engineering Microbiology for Bast Fiber Crops,Ministry of Agriculture,Changsha 410205,China;2Crop Gene Engineering Key Laboratory of Hunan Province,Hunan Agricultural University,Changsha 410128,China
  • Received:2008-11-13 Revised:2009-03-20 Online:2009-08-12 Published:2009-06-10
  • Contact: CHEN Xin-Bo，E-mail:chenxinbo@hotmail.com;Tel:0731-8998533

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Abstract:

Flax (Linum usitatissimum) is an important bast fiber crop. High lignin content in the flax fiber greatly affects the performance and quality in the flax textile industry. CCoAOMT1, 4CL1, 4CL2, COMT, F5H1, F5H2,and F5H3 are putative genes coding the key enzymes involved in lignin accumulation. Expression patterns of these genes were analyzed by semi-quantitative RT-PCRin different flax tissues and in xylem and phloem (bark) at different development stages of the variety Baihua. The results revealed that All genes were detected to express in seedlings, roots, florescent xylem, florescent phloem, leaves, flowers and young fruits excluding F5H1 in roots. Expression of CCoAOMT1 was consistently high in all the tissues detected. There existed complementary expression between two 4CL genesand among the three F5H genes in various tissues. In the phloem, all genes lowly expressed except for 4CL2 at florescence and highly expressed after flowering. Expression of 4CL1 was high, and that of F5H3 was low in the phloem at all stages compared with that of other genes. In the xylem, expression of 4CL1 gene was low at all the developing stages, that of F5H1 reached a peak before flowering and that of the other genes in the xylem reached the highest at 30 d after emergence of seedlings and gradually declined at late growing stages, with a sharp decrease to very lower level after flowering. The expression of 4CL1compared with other genes was significantly higher in phloem than in xylem.

Key words: Flax, Lignin, Expression analysis, Semi-quantitative PT-PCR


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