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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1771-1777.doi: 10.3724/SP.J.1006.2009.01771


Transgenic Ramie with Bt Gene  Mediated by Agrobacterium tumefacien and Evaluation of Its Pest-Resistance

FU Jia-Ping,WANG Bo**,LIU Li-Jun,YANG Jin-Yu,WANG Xu-Xia,XING Xiu-Long,PENG Ding-Xiang*   

  1. College of Plant Sciences and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2009-03-03 Revised:2009-06-25 Online:2009-10-12 Published:2009-08-07
  • Contact: PENG Ding-Xiang,E-mail: pdxiang@mail.hzau.edu.cn; Tel: 13397121298


Agrobacterium tumefaciens-mediated approach is an effectively and widely used to introduce foreign DNA into plants. However, there were few reports published on ramie transformation mediated by Agrobacterium tumefaciens up to date. In the present study, transgenic ramie containing Bt gene were obtained by our highly efficient Agrobacterium-mediated transformation system using ramie Luzhuqing. Three T0 plants of transgenic ramie were chosen for further experiments and were transplanted into the fields. Laboratory assay of artificial infestation and the survey on agronomic traits and quality traits of T0 plants were conducted. The results showed that T0 transgenic plants exhibited more excellent pest resistance than the controls, and some were significantly superior to the controls. In addition, T0 transgenic plants maintained the main agronomic traits, such as plant height, stem diameter, rate of effective tiller, raw fiber weight, fiber production ratio from stem and fiber production ratio from phloem, and quality traits, such as strength, filament breaking tenacity, fiber fineness, fineness uniformity and glue content of raw fiber in Luzhuqing. PCR and Southern blotting analysis showed that T1 plants contained Bt gene, which indicated that Bt gene could be stably inherited. T1 plants in the fields performed highly pest resistance compared with non-transgenic plants.

Key words: Ramie, Transformation, Bt gene, Pyrameis indica(HerBst), Agrobaterium tumefaciens

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