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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1936-1941.doi: 10.3724/SP.J.1006.2009.01936

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Genetic Diversity Analysis of Black Sesame(Sesamum indicum DC) Core Collection of China Using SRAP Markers

CHE Zhuo1,2,ZHANG Yan-Xin2,**,SUN Jian2,ZHANG Xiu-Rong2,*,SHANG Xun-Wu1,WANG Hua-Jun1   

  1. 1 Gansu Agricultural University / Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement, Lanzhou 730070, China; 2 Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2009-01-14 Revised:2009-04-23 Online:2009-10-12 Published:2009-08-07
  • Contact: ZHANG Xiu-Rong，E-mail: zhangxr@oilcrops.cn

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Abstract:

China has a long history and rich germplasm resources for growing black sesame. Black sesame is well used because of its nutrition superior to black rice and black soybean. However, Research on black sesame is relatively less than that on other crops. In the paper, genetic diversity of black sesame core collection of China was analyzed using SRAP (sequence-related amplified polymorphism) markers. The results showed that 13 SRAP primer combinations were employed to evaluate the genetic diversity of 100 black sesame accessions, a total of 182 amplified fragments were detected and 126 of them were polymorphic, the polymorphism percentage was 69.2%, the number of amplified fragments and polymorphic fragments of each primer combination were 14.0 and 9.7, respectively. The 100 accessions were grouped into five clusters at genetic similarity of 0.68, indicating the genetic diversity of these accessions was abundant. As regards geographic regions, Shannon's information index of black sesame accessions in south China (0.3557) was the highest，the followings were that in central regions of China (0.3415) and north China (0.2986). The characteristics of genetic diversity of black sesame core collection of China were fully revealed in this study, which provided the theoretical foundations for further exploring, collecting and introducing black sesame germplasm as well as mining and utilizing excellent black sesame germplasm in the future.

Key words: Black sesame, Core collection, SRAP, Genetic diversity

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