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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1942-1947.doi: 10.3724/SP.J.1006.2009.01942

• RESEARCH ACTIVITIES • Previous Articles    

In silico Mapping and Structure Analysis of Key Enzyme Genesis in Fatty Acid Synthesis of Soybean

SONG Wan-Kun1,2,ZHU Ming-Xi1,2,ZHAO Yang-Lin1,WANG Jing1,LI Wen-Fu1,LIU Chun-Yan1,2,CHEN Qing-Shan2,*,HU Guo-Hua1,3,*   

  1. 1The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,China;2Agriculture College,Northeast Agricultural University,Harbin 150030,China;3The National Research Center of Soybean Engineering and Technology,Harbin 150050,China
  • Received:2009-01-15 Revised:2009-04-26 Online:2009-10-12 Published:2009-07-04
  • Contact: HU Guo-hua,E-mail:hugh757@vip.163.com;CHEN Qing-Shan,E-mail:qshchen@126.com;Tel:0451-55191945

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Abstract:

The 12 genes of key enzyme such as acyl-CoA carboxylase and fatty acid synthase in fatty acid synthesis were mapped on the newest genetic map integrating from physical map and genetic map, and the gene structure was analyzed. They were mapped on nine linkage groups, including A2, B2, C2, D1b, D2, G, I, L, M, and the flank markers of the gene on the linkage groups were obtained. At the same time, we compared the sequence information of cDNA with gDNA to get the structure information of the 12 genes, with the number of intron from 0 to 30. FAD2-1, FAD2-2, FAD2-3, and FatB were all single extron gene, but there were six introns in KASI, twelve introns in KASII, two introns in SACPD, and seven introns in FAD3. So the corresponding markers obtained from the mapping are available for molecular assisted selection, while the structure information can be better used in gene function analysis.

Key words: Soybean, Fatty acid, Gene, In silico mapping, Gene structure

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