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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (4): 565-573.doi: 10.3724/SP.J.1006.2010.00565

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Mutants from Adzuki Bean (Vigna angularisi) Jingnong 6 seed Induced by Physical and Chemical Agents

TONG Xing1,ZHAO Bo1,JING Wen-Lin1,ZENG Chao-Wu1,2,LIU Hong-Xia1,2,WU Bao-Mei1,PU Shao-Jing1,CHEN Xue-Zhen1,PAN Jin-Bao1,WAN Ping1*   

  1. 1 College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2009-09-27 Revised:2010-01-09 Online:2010-04-12 Published:2010-02-09
  • Contact: FANG Ping, E-mail: pingwan3@yahoo.com.cn

Abstract:

Mutational approaches have been widely exploited in breeding, genetics and gene function researches. We firstly developed a large collection of mutants from adzuki bean (Vigna angularisi) cultivar Jingnong 6 treated by 0.5%, 0.9%, and 1.4% EMS for 12 h and 24 h, 400 Gy 60Co-γ ray and 100 Gy, 300 Gy, and 600 Gy electron beam. The results indicated that the maximum mutantions were induced by 0.5% or 0.9% EMS for 24 h. More seed color mutants including cream-colored and light red seeds, pod color mutants such as brown, dark brown and black pods were produced by o.5% EMS. Needle leaf, sword, kidney-shaped and small heart-shaped leaf mutants were obtained by 0.9% EMS treatment. The percentage of mutants with 100, 300, and 600 Gy electron beam treatment were 4.09%, 3.64%, and 2.22% respectively. The percentage of mutants treated by 400 Gy 60Co-γ ray was 7.23%. Nine hundred thirty-seven EMS-induced M3 lines and nine hundred thirty-four M2 lines radiated by 60Co-γ ray and electron beam were generated. A total of 1 490 mutants were collected. Mutations included in plant height, plant architecture, leaf shape and color, leaf size, seed shape and color, seed size, pod color, branching type and number, bushy leaf or branch, sprawl, definite growth, sterile, early and late mature, flowering time. The mutant populations are very useful to genetic analysis of gene, gene mapping and cloning, and further the research on functional genomics. These mutants will be useful to serve the adzuki bean improvement.

Key words: Adzuki bean(Vigna angularisi), EMS and irradiation mutagenesis, Mutants, Mutant identification

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