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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1223-1230.doi: 10.3724/SP.J.1006.2013.01223

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Fine Mapping of a Lesion Mimic Mutant spl31 in Rice

DAI Gao-Meng,ZHU Xiao-Yan,LI Yun-Feng,LING Ying-Hua,ZHAO Fang-Ming,YANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China
  • Received:2012-11-16 Revised:2013-01-15 Online:2013-07-12 Published:2013-04-23
  • Contact: 何光华,E-mail: hegh@swu.edu.cn

Abstract:

A lesion mimic mutant temporarily designated as spl31 was discovered in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by ethyl methane sulfonate (EMS). The mutant phenotype was normal before the three leaf period but with yellow bad spots onleaves after the four leaf period. Along with the growth and development of the plant, the number of spots didn’t show significant difference, whereas the lesion area gradually enlarged and developed with tawny margin. As the spl31 matured, lesion spots interconnected between each other becoming a continuous piece, resulting in leaves’ death. The observation by transmission electronic microscope (TEM) demonstrated that most grana lamellae in spl31’s chroloplasts were irregularly stacked. Histochemical analysis revealed that deep blue stained cells presented discrete dietilution, illustrating that the lesion mechanism of spl31 may be spontaneously generated. The photosynthetic data showed that the mutation of spl31 had no effect onPSII. Furthermore, both 1000-grain weight and seed setting rate of the mutant decreased as compared with the wild type. Genetic analysis suggested that spl31 was controlled by a single nuclear recessive gene. Nipponbare was crossed with the spl31 and 621 mutational F2 recessive plants were used for gene mapping. And finally, Spl31 locus was mapped on chromosome 12 near the centromere between ID104 and ID11 with physical distance of 383 kb and co-segregated with ID105.

Key words: Oryza sativa L., Lesion mimic mutants, Programmed cell death, Gene mapping

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