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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 650-660.doi: 10.3724/SP.J.1006.2011.00650

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Leaf Number of Stock or Scion in Graft Union on Scion Growth and Development of Soybean

JIA Zhen1,2,WU Cun-Xiang1,WANG Miao1,SUN Hong-Bo1,HOU Wen-Sheng1,JIANG Bing-Jun1,HAN Tian-Fu1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Tianshui Normal College, Tianshui 741001, China
  • Received:2010-11-03 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 韩天富, E-mail: hantf@mail.caas.net.cn, Tel: 010-82108784

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Abstract: To elucidate whether there exist floral stimuli and inhibitors in flowering process, what the underlying mechanism is, and how they regulate plant growth and development, we used the early-maturing soybean cultivar Heihe 27 and late-maturing cultivar Zigongdongdou as stock and scion, respectively to make graf in which scions and stocks had different leaf numbers in combination with defoliation, and to study the effect of leaf number of stock and scion on the reproductive development. Under long-day (16 h) conditions, Zigongdongdou scions flowered at day 30 after being grafted onto Heihe 27 stocks, while its self-grafts failed to flower until the experiment finished (over 120 days). It suggested that under noninductive photoperiods (long-day), the transmissible floral stimuli were produced in the leaves of the early-maturing Heihe 27 and moved to the apical meristem of the late-maturing Zigongdongdou to induce the scion to flower. And the effect of the stock on the flowering of the scion was dependent on the number of leaves remained in the stock. It indicated that there exists an accumulative effect of the transmissible stimuli on soybean flowering. Defoliation on scions promoted flowering and increased flower number, which showed that some floral inhibitors were produced in the leaves of scions under long-day conditions. The number of flowers and pods on scions negatively correlated with the number of remained scion leaves. It indicated that the floral inhibitors suppress the development of the reproductive organs, and the inhibitions have an accumulative effect as well. The node location where scions were defoliated also had an effect on the flowering development. It was proposed that, during the growth and development of soybean, leaves regulate the amount and balance of floral stimuli and inhibitors based on photoperiodic signals, consequently trigger the proceeding from vegetative growth to the reproductive development to ensure the completion of life cycle.

Key words: Soybean, Graft, Maintained leaf number, Transmissible floral substance, Accumulative effect

[1]Knott J E. Effect of localized photoperiod on Spinach. Proc Am Soci Hort Sci, 1934, 31: 152-154
[2]Chailakhyan M Kh. New facts in support of the hormonal theory of plant development. Comp Rend de l'Académie des Sci de l'URSS, 1936, 13: 79-83
[3]Chailakhyan M Kh. Gormonal’naya Teoriya Razvitiya Rastenii (Hormonal Theory of Plant Development), Moscow: Akad. Nauk SSSR, 1937
[4]Lang A. Physiology of flower initiation. Ruhland W. ed, Encycl. Plant Physiol, Berlin: Springer-Verlag, 1965, XV/1: 1380-1536
[5]Zeevaart J A. Physiology of flower formation. Annu Rev Plant Physiol, 1976, 27: 321-348
[6]Hamner K C, Bonner J. Photoperiodism in relation to hormones as factors in floral initiation and development. Bot Gaz, 1938, 100: 388-431
[7]Yukiyoshi O, Roderick W K. The inhibition of flowering by non-induced cotyledons of Pharbitis nil. Plant Cell Physiol, 1990, 31: 129-135
[8]Lang A, Chailakhyan M K, Frolova I A. Promotion and inhibition of flower formation in a dayneutral plant in grafts with a short-day plant and a long-day plant. Proc Natl Acad Sci USA, 1977, 74: 2412-2416
[9]Zeevaart J A. Florigen coming of age after 70 years. Plant Cell, 2006, 18: 1783-1789
[10]Lang A, Melchers G. Die photoperiodische reaktion von Hyoscyamus niger. Planta, 1943, 33: 653-702
[11]Evans L T. Inflorescence initiation in Lolium temulentum L. II. Evidence for inhibitory and promotive photoperiodic processes involving transmissible products. Proc Amer Soc Hort Sci, 1960, 13: 429-440
[12]Thompson P A, Guttridge C G. The role of leaves as inhibitors of flower induction in strawberry. Ann Bot, 1960, 24: 482-490
[13]Raghavan V, Jacobs W P. Studies on the floral histogenesis and physiology of perilla in floral induction in cultured apical buds of P. frutescens. Am J Bot, 1961, 48: 751-760
[14]Weller J L, Murfet I C, Reid J B. Pea mutants with reduced sensitivity to far-red light define an important role for phytochrome A in day-length detection. Plant Physiol, 1997, 114: 1225-1236
[15]Garner W W, Allard H A. Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants. J Agric Res, 1920, 18: 553-606
[16]Borthwick H A, Parker M W. Photoperiodic perception in Biloxi soybean. Bot Gaz, 1938, 100: 374-387
[17]Hamner K C. Interrelation of light and darkness in photoperiodic induction. Bot Gaz, 1940, 101: 658-687
[18]Parker M W, Hendricks S B, Borthwick H A, Scully N J. Action spectrum for the photoperiodic control of floral initiation of short-day plants. Bot Gaz, 1946, 108: 1-26
[19]Coulter M W, Hamner K C. Photoperiodic flowering response of Biloxi soybean in 72-hour cycles. Plant Physiol, 1964, 39: 848-856
[20]Hamner K C, Takimoto A. Circadian rhythms and plant photoperiodism. Am Nat, 1964, 98: 295-322
[21]Heinze P H, Parker M W, Borthwick H A. Floral initiation in Biloxi soybean as influenced by grafting. Bot Gaz, 1942, 103: 518-530
[22]Kiyosawa S, Kiyosawa K. A study on varietal difference in flowering habits of soybean plants as followed by grafting experiments. Plant Cell Physiol, 1962, 3: 263-273
[23]Kiihl R A S, Hartwig E E, Kilen T C. Grafting as a tool in soybean breeding. Crop Sci, 1977, 17: 181-183
[24]Beaver J S, Nelson R L. Effect of grafting date and maturity of the stock on the flowering behavior of soybean scions. Soybean Genet Newsl, 1981, 8: 37-40
[25]Sinclair T R, Hinson K. Soybean flowering in response to the long-juvenile trait. Crop Sci, 1992, 32: 1242-1248
[26]Cober E R, Curtis D F. Both promoters and inhibitors affected flowering time in grafted soybean flowering-time isolines. Crop Sci, 2003, 43: 886-891
[27]Przepiorkowski T, Martin S K S. The effect of grafting on the flowering of near-isogenic lines of soybean. Crop Sci, 2003, 43: 1760-1763
[28]Jia Z(贾贞), Han T-F(韩天富). Uses of graft techniques in the studies of physiology and breeding of soybean. Soybean Sci (大豆科学). 2010, 29(1): 136-142 (in Chinese with English abstract)
[29]Han T-F(韩天富), Wang J-L(王金陵). Studies on the post flowering photoperiodic responses in soybean. Acta Bot Sin (植物学报), 1995, 37(11): 863-869 (in Chinese with English abstract)
[30]Han T-F(韩天富), Gai J-Y(盖钧镒), Wang J-L(王金陵), Zhou D-X(周东兴). Discovery of flowering reversion in soybean plants. Acta Agron Sin (作物学报), 1998, 24(2): 168-172 (in Chinese with English abstract)
[31]Washburn C F, Thomas J F. Reversion of flowering in Glycine max (Fabaceae). Am J Bot, 2000, 87: 1425-1438
[32]Wu C, Ma Q, Yam K-M, Cheung M-Y, Xu Y, Han T, Lam H-M, Chong K. In situ expression of the GmNMH7 gene is photoperiod-dependent in a unique soybean (Glycine max
[L.] Merr.) flowering reversion system. Planta, 2006, 223: 725-735
[33]Battey N, Lyndon R. Reversion of flowering. Bot Rev, 1990, 56: 162-189
[34]Han T, Wu C, Tong Z, Mentreddy R S, Tan K, Gai J. Postflowering photoperiod regulates vegetative growth and reproductive development of soybean. Environ Exp Bot, 2006, 55: 120-129
[35]Fehr W R, Caviness C E. Stages of Soybean Development. Special Report 80, Cooperative Extension Service, Agriculture and Home Economic Experiment Station. Ames, Iowa: Iowa State University, 1977. pp 1-11
[36]Tang Q-Y(唐启义), Feng M-G(冯明光). DPS Data Processing System—Experimental Design, Statistical Analysis and Data Mining (DPS数据处理系统—实验设计、统计分析及数据挖掘). Beijing: Science Press, 2007 (in Chinese)
[37]Shanmugasundaram S, Wang C-C, Toung T S. Photoperiodic response of flowering in two-branched soybean plants. Bot Gaz, 1979, 140: 4141-4147
[38]Liu H, Wang H, Gao P, Xu J, Xu T, Wang J, Wang B, Lin C, Fu Y F. Analysis of clock gene homologs using unifoliolates as target organs in soybean (Glycine max). J Plant Physiol, 2009, 166: 278-289
[39]Corbesier L, Vincent C, Jang S, Fornara F, Fan Q, Searle I, Giakountis A, Farrona S, Gissot L, Turnbull C, Coupland G. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science, 2007, 316: 1030-1033
[40]Jaeger K E, Wigge P A. FT protein acts as a long-range signal in Arabidopsis. Curr Biol, 2007, 17: 1050-1054
[41]Mathieu J, Warthmann N, Kuttner F, Schmid M. Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis. Curr Biol, 2007, 17: 1055-1060
[42]Notaguchi M, Abe M, Kimura T, Daimon Y, Kobayashi T, Yamaguchi A, Tomita Y, Dohi K, Mori M, Araki T. Long-distance, graft-transmissible action of Arabidopsis FLOWERING LOCUS T protein to promote flowering. Plant Cell Physiol, 2008, 49: 1645-1658
[43]Tamaki S, Matsuo S, Wong H L, Yokoi S, Shimamoto K. Hd3a protein is a mobile flowering signal in rice. Science, 2007, 316: 1033-1036
[44]Lin M K, Belanger H, Lee Y J, Varkonyi-Gasic E, Taoka K, Miura E, Xoconostle-Cazares B, Gendler K, Jorgensen R A, Phinney B, Lough T J, Lucas W J. FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the cucurbits. Plant Cell, 2007, 19: 1488-1506
[45]Lifschitz E, Eshed Y. Universal florigenic signals triggered by FT homologues regulate growth and flowering cycles in perennial day-neutral tomato. J Exp Bot, 2006, 57: 3405-3414
[46]Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T. A pair of related genes with antagonistic roles in mediating flowering signals. Science, 1999, 286: 1960-1962
[47]Hanzawa Y, Money T, Bradley D. A single amino acid converts a repressor to an activator of flowering. Proc Natl Acad Sci USA, 2005, 102: 7748-7753
[48]Ahn J H, Miller D, Winter V J, Banfield M J, Lee J H, Yoo S Y, Henz S R, Brady R L, Weigel D. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J, 2006, 25: 605-614
[49]Kardailsky I, Shukla V K, Ahn J H, Dagenais N, Christensen S K, Nguyen J T, Chory J, Harrison M J, Weigel D. Activation tagging of the floral inducer FT. Science, 1999, 286: 1962-1965
[50]Ohshima S, Murata M, Sakamoto W, Ogura Y, Motoyoshi F. Cloning and molecular analysis of the Arabidopsis gene TERMINAL FLOWER 1. Mol Gen Genet, 1997, 254: 186-194
[51]Foucher F, Morin J, Courtiade J, Cadioux S, Ellis N, Banfield M J, Rameau C. DETERMINATE and LATE FLOWERING are two TERMINAL FLOWER1/CENTRORADIALIS homologs that control two distinct phases of flowering initiation and development in pea. Plant Cell, 2003, 15: 2742-2754
[52]Pillitteri L J, Lovatt C J, Walling L L. Isolation and characterization of a TERMINAL FLOWER homolog and its correlation with juvenility in citrus. Plant Physiol, 2004, 135: 1540-1551
[53]Conti L, Bradley D. TERMINAL FLOWER1 is a mobile signal controlling Arabidopsis architecture. Plant Cell, 2007, 19: 767-778
[54]Liu B, Watanabe S, Uchiyama T, Kong F, Kanazawa A, Xia Z, Nagamatsu A, Arai M, Yamada T, Kitamura K, Masuta C, Harada K, Abe J. The soybean stem growth habit gene Dt1 is an ortholog of Arabidopsis TERMINAL FLOWER1. Plant Physiol, 2010, 153: 198-210
[55]Tian Z, Wang X, Lee R, Li Y, Specht J E, Nelson R L, McClean P E, Qiu L, Ma J. Artificial selection for determinate growth habit in soybean. Proc Nat Acad Sci USA, 2010, 107: 8563-8568
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