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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 650-660.doi: 10.3724/SP.J.1006.2011.00650

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Leaf Number of Stock or Scion in Graft Union on Scion Growth and Development of Soybean

JIA Zhen1,2,WU Cun-Xiang1,WANG Miao1,SUN Hong-Bo1,HOU Wen-Sheng1,JIANG Bing-Jun1,HAN Tian-Fu1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Tianshui Normal College, Tianshui 741001, China
  • Received:2010-11-03 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 韩天富, E-mail: hantf@mail.caas.net.cn, Tel: 010-82108784

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Abstract: To elucidate whether there exist floral stimuli and inhibitors in flowering process, what the underlying mechanism is, and how they regulate plant growth and development, we used the early-maturing soybean cultivar Heihe 27 and late-maturing cultivar Zigongdongdou as stock and scion, respectively to make graf in which scions and stocks had different leaf numbers in combination with defoliation, and to study the effect of leaf number of stock and scion on the reproductive development. Under long-day (16 h) conditions, Zigongdongdou scions flowered at day 30 after being grafted onto Heihe 27 stocks, while its self-grafts failed to flower until the experiment finished (over 120 days). It suggested that under noninductive photoperiods (long-day), the transmissible floral stimuli were produced in the leaves of the early-maturing Heihe 27 and moved to the apical meristem of the late-maturing Zigongdongdou to induce the scion to flower. And the effect of the stock on the flowering of the scion was dependent on the number of leaves remained in the stock. It indicated that there exists an accumulative effect of the transmissible stimuli on soybean flowering. Defoliation on scions promoted flowering and increased flower number, which showed that some floral inhibitors were produced in the leaves of scions under long-day conditions. The number of flowers and pods on scions negatively correlated with the number of remained scion leaves. It indicated that the floral inhibitors suppress the development of the reproductive organs, and the inhibitions have an accumulative effect as well. The node location where scions were defoliated also had an effect on the flowering development. It was proposed that, during the growth and development of soybean, leaves regulate the amount and balance of floral stimuli and inhibitors based on photoperiodic signals, consequently trigger the proceeding from vegetative growth to the reproductive development to ensure the completion of life cycle.

Key words: Soybean, Graft, Maintained leaf number, Transmissible floral substance, Accumulative effect

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