水稻细卷叶突变体nrl2(t)的遗传分析和基因定位

doi:10.3724/SP.J.1006.2011.01159

Abstract

Abstract: Study of the genes which regulate leaf development is significant in rice functional genomics research and molecular breeding. In the present study, a novel rolled and narrow leaf mutant was obtained from the rice (Oryza sativa L.) restorer line Jinhui 10 treated by ethyl methyl sulphonate (EMS), named nrl2_(t). The mutant exhibited narrow/semi-rolled and elongate leaves, earlier flowering, thinner stalk, higher pigment content and higher plant height compared with the control. The indole-3-acetic acid (IAA) content relatively reduced in the flag leaf compared to that of wild type, but increased in the young panicle at the heading stage. Genetic analysis indicated that the mutant was controlled by a single recessive gene, located on the chromosome 3 between SSR markers s3RM1 and s3RM3, delimiting to a 114 kb region. These results will lay a good foundation for molecular cloning and functional analysis of the NRL2_(t).

Key words: Rice (Oryza sativa L.), Narrow and rolled leaf, Genetic analysis, Gene mapping, IAA

WANG De-Zhong, SANG Xian-Chun, LIU Xiao-Qiang, WANG Ceng, WANG Qiu-Shi, DIAO Fang-Meng, LING Yang-Hua, LI Yun-Feng, HE Guang-Hua. Genetic Analysis and Gene Mapping of a Novel Narrow and Rolled Leaf Mutant nrl2(t) in Rice (Oryza sativa L.)[J].Acta Agron Sin, 2011, 37(07): 1159-1166.

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Genetic Analysis and Gene Mapping of a Novel Narrow and Rolled Leaf Mutant nrl2(t) in Rice (Oryza sativa L.)

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