小麦BNS雄性不育系及其转换系花药差异蛋白鉴定与分析

doi:10.3724/SP.J.1006.2011.01540

Abstract

Abstract: BNS is a new type of thermo-sensitive nuclear male-sterile lines in wheat (Triticum aestivum L.). The mechanism of itssterility was studied using two-dimensional electrophoresis and MALDI-TOF-MS methods. Anthers of the sterile line (SL) and the conversion line (CL, fertile pollen rate higher than 50%) were obtained from plants with a series seeding dates, and two set of samples were used to compare the differentially expressed proteins which was extracted from the anthers at early stage of uninucleate and from late stage of uninucleate to binucleate stage of pollen development. The abundant expressions of proteins involved in respiratory and photosynthetic energy metabolism were identified in the CL, such as ATP synthase α and β subunit, NAD (P) Rossmann fold binding protein, cytoplasmic malate dehydrogenase, mitochondrial aldehyde dehydrogenase subunit, and Rubisco subunit protein. However, they were absent or down-regulated in the SL. Besides, a few abnormal proteins were detected in the SL, including sorbitol dehydrogenase, maturase K, histone H2B.2, Harpin-induced 1, and elongation factor TU. According to the functions of these differentially expressed proteins, we inferred that ATP synthase α and β subunits were most likely the source protein for the sterility of BNS. Their expressions are regulated by a temperature sensor that is located at upstream of the subunit gene operon in BNS. Mutation of the temperature sensor is determined by the temperature higher than a threshold, which may startup the transcriptions of α and β subunit genes. When the temperature is lower than the threshold, the expressions of α and β subunits are in very low levels, which affect the assembly of ATP synthase, and result in deficiency of ATP supply for the growth of microspore. This effect can be amplified in downstream physiological functions, such as development of respiratory and photosynthetic enzymes, which finally causes abnormal metabolism and pollen sterility.

Key words: Wheat BNS male-sterility, Anther differentially expressed proteins, Two-dimensional gel electrophoresis, MALDI-TOF-MS, ATP synthase

LI You-Yong, RU Zhen-Gang, SU Qing, FU Qing-Yun. Identification and Analysis of Differentially Expressed Proteins of BNS Male Sterile Line and Its Conversion Line of Wheat[J].Acta Agron Sin, 2011, 37(09): 1540-1550.

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Identification and Analysis of Differentially Expressed Proteins of BNS Male Sterile Line and Its Conversion Line of Wheat

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