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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (07): 1212-1218.doi: 10.3724/SP.J.1006.2011.01212

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Proteomic Analyses of the Maize Cross Incompatibility Gene Ga1-S

LIU Huai-Hua,WANG Li-Wen**,LIU Xu,MA Xia,NING Li-Hua,ZHANG Hua,CUI De-Zhou,JIANG Chuan,CHEN Hua-Bang*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College, Shandong Agricultural University, Tai’an 271018, China
  • Received:2010-11-16 Revised:2011-03-28 Online:2011-07-12 Published:2011-04-12
  • Contact: 陈化榜, E-mail: hbchen@sdau.edu.cn, Tel: 0538-8249939

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Abstract: The objective of this paper was to study the cross incompatibility gene Ga1-S in maize through proteomic approach. Near isogonic lines of Ga1-S gene, W22 (GG) and w22 (gg), were used to make the crosses of GG×GG, gg×GG,and GG×gg respectively. First，we observed the growth process of pollen tubes grown into silks in three crosses by fluorescence microscopy; second, total silk proteins were extracted from silks of the W22 (GG) at 10 h after pollination. Total proteins were extracted by TCA/Acetone, separated by two-dimensional gel electrophoresis (2-DE) and analyzed through MALDI-TOF-MS mass spectrometry. The results indicated that pollen tube couldn’t grow into silks base in the cross of GG×gg, but could in the other two crosses; In the silk proteome of GG×GG and GG×gg , there were 25 differentially expressed proteins, including 15 specifically expressed in GG×GG, and 10 specifically expressed in GG×gg. And 12 of them were annotated in various databases by MALDI-TOF-MS and MASCOT analyses. Proteins 11, 12, 14, 18, 22 and 24 presumably play important roles in the maize cross incompatibility.

Key words: Zea mays L., Cross incompatibility, Ga1-S, Proteome, 2-DE, MALDI-TOF-MS

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