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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1839-1846.doi: 10.3724/SP.J.1006.2012.01839


Cloning and Expression Analysis of Heat Shock Protein Gene ZmHSP90-1 in Maize

LIU Ling-Ling1,2,LIU Si-Si2,4,WENG Jian-Feng2,WANG Chang-Tao3,LI Xin-Hai2,ZHANG Shi-Huang2,SHI Qing-Hua4,WANG Li-Juan1,*,HAO Zhuan-Fang2,*   

  1. 王丽娟, E-mail: lj-wang@163.com; 郝转芳, E-mail: haozhuanfang@yahoo.com.cn
  • Received:2012-03-12 Revised:2012-04-20 Online:2012-10-12 Published:2012-07-03


The heat shock protein 90 (HSP90) is a widespread family of molecular chaperones found in prokaryotes and all eukaryotes, with high conservation among plant species. In this study, a HSP90 gene was isolated from maize, named ZmHSP90-1. The full cDNA sequence of ZmHSP90-1 is 2 371 bp, containing a 2 094 bp open reading frame (ORF) and encoding 697 amino acids with a predicted molecular mass of 79.98 kD. The ZmHSP90-1 protein contains a predicted ATPase site and a HSP90 conservative structure domain, which is highly conserved in plants and especially similar to AtHSP90.1. Instantaneous expression analysis showed that ZmHSP90-1 proteins was localized in cytoplasm. The expression of ZmHSP90-1 in maize analyzed by quantitative real-time PCR was induced by hot, high-salt, ABA, cold and drought treatments. These results suggested that ZmHSP90-1 might be a stress related gene of maize.

Key words: Maize (Zea mays L.), Heat shock protein, Abiotic stresses, ZmHSP90-1

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