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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1833-1838.doi: 10.3724/SP.J.1006.2012.01833

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of GmPGIP3 Transgenic Wheat Yangmai 18 with Enhanced Resistance to Common Root Rot

DANG Liang1,2,**,WANG Ai-Yun1,3,**,XU Hui-Jun1,ZHU Xiu-Liang1,DU Li-Pu1,SHAO Yan-Jun2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Biology Science, Agricultural University of Hebei, Baoding 071001, China; 3 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
  • Received:2012-04-19 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn

Abstract:

GmPGIP3 is a polygalacturonase-inhibiting protein from soybean, which could reduce the infection of fungal pathogen throughr inhibiting the endo-polygalacturonase activity of pathogen fungi. Using genetic recombination technique, we constructed the transformation vector of GmPGIP3 gene expressing highly in monocot plants, pA25-GmPGIP3, in which GmPGIP3 gene was driven by maize ubiquitin promoter. Embryo callus of Yangmai 18 was bombarded by the particle containing pA25-GmPGIP3 vector DNA. The GmPGIP3 transgenic wheat plants from T0 to T2 generations were detected by PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. The alien GmPGIP3 proved to be introduced into seven transgenic wheat lines with heritability and expression events. We also evaluated the disease resistance in these GmPGIP3 transgenic plants through inoculating the common root rot pathogen, Bipolaris sorokiniana. Compared with untransformated Yangmai 18, five GmPGIP3 transgenic lines showed significantly-enhanced resistance to Bipolaris sorokiniana.

Key words: Polygalacturonase-inhibiting protein (PGIP), GmPGIP3, Transgenic wheat, Molecular detection, Common root rot, Resistance

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