Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (07): 1219-1228.doi: 10.3724/SP.J.1006.2011.01219

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Dissecting and QTL Mapping of Component Traits of Resistance to Wheat Powdery Mildew at Early Infection Stage

WANG Hua-Zhong,ZHANG Zhen,HE Yang,YUE Jie-Yu   

  1. School of Life Sciences / Tianjin Key Laboratory of Cyto-genetical & Molecular Regulation, Tianjin Normal University, Tianjin 300387, China
  • Received:2010-11-02 Revised:2011-03-06 Online:2011-07-12 Published:2011-04-12

PDF

1507

Abstract: Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of the major fungal diseases of wheat, and the use of resistant cultivars is the most effective disease control approach. Here, the International Triticeae Mapping Initiative (ITMI) W7984 × Opata85 wheat recombinant inbred population was used for powdery mildew resistance genetic study. Resistance complex trait was dissected into component traits as papilla index (PI), papilla scale (PC), haustorium index (HI) and secondary hypha index (SI) of several successive timepoint at early stage of Bgt infection. The change trends of PI and HI with time were both controlled by single main effect genes. With quantitative trait analysis, 34 resistance-related QTLs including 21 main effect QTLs were found and distributed in wheat 1B, 1D, 2B, 3A, 3B, 3D, 4A, 4B, 4D, 5B, 6A, 6B, 6D, 7B, and 7D chromosomes. QTL QPmPI16.tn-7B, which regulated the formation of papilla, had the largest effect, singly accounting for 48.69% of the phenotypic variance. Locus of QPmPI16.tn-7B promoting papilla formation was derived from the parent Opata85. Colocalized QTLs were found among different component traits. Component trait-specific QTLs may provide more information about the genetic mechanism of wheat powdery mildew resistance.

Key words: Wheat powdery mildew, Early stage of infection, Component traits, QTL mapping

[1]Vanderplank J E. Plant Disease: Epidemics and Control. New York: Academic Press, 1963
[2]Lillemo M, Asalf B, Singh R, Huerta-Espino J, Chen X, He Z, Bjørnstad A. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet, 2008, 116: 1155–1166
[3]Miranda L M, Murphy J P, Marshall D, Cowger C, Leath S. Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 1451–1456
[4]Miranda L M, Murphy J P, Marshall D, Leath S. Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet, 2006, 113: 1497–1504
[5]Zhu Z D, Zhou R H, Kong X Y, Dong Y C, Jia J Z. Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat. Genome, 2005, 48: 585–590
[6]Hautea R A., Coffman W R, Sorrells M E, Bergstrom G C. Inheritance of partial resistance to powdery mildew in spring wheat. Theor Appl Genet, 1987, 73: 609–615
[7]Shaner G. Evaluation of slow-mildewing resistance of Knox wheat in the field. Phytopathology, 1973, 63: 867–872
[8]Gustafson G D, Shaner G. The influence of plant age on the expression of slow-mildewing resistance in wheat. Phytopathology, 1982, 72: 746–749
[9]Griffey C A, Das M K, Stromberg E L. Effectiveness of adult-plant resistance in reducing grain yield loss to powdery mildew in winter wheat. Plant Dis, 1993, 77: 618–622
[10]Griffey C A, Das M K. Inheritance of adult-plant resistance to powdery mildew in Knox62 and Massey winter wheats. Crop Sci, 1994, 34: 641–646
[11]Keller M, Keller B, Schachermayr G, Winzeler M, Schmid J E, Stamp P, Messmer M M. Quantitative trait loci for resistance against powdery mildew in a segregating wheat × spelt population. Theor Appl Genet, 1999, 98: 903–912
[12]Liu S X, Griffey C A, Saghai Maroof M A. Identification of Molecular Markers Associated with Adult Plant Resistance to Powdery Mildew in Common Wheat Cultivar Massey. Crop Sci, 2001, 41: 1268–1275
[13]Chantret N, Mingeot D, Sourdille P, Bernard M, Jacquemin J M, Doussinault G. A major QTL for powdery mildew resistance is stable over time and at two development stages in winter wheat. Theor Appl Genet, 2001, 103: 962–971
[14]Hu G, Webb C A, Hulbert S H. Adult plant phenotype of the Rp1–DJ compound rust resistance gene in maize. Phytopathology, 1997, 87: 236–241
[15]Penning, B W, Johal G S, McMullen M D. A major suppressor of cell death, slm1, modifies the expression of the maize (Zea mays L.) lesion mimic mutation les23. Genome, 2004, 47: 961–969
[16]Andaya C B, Ronald P C. A catalytically impaired mutant of the rice Xa21 receptor kinase confers partial resistance to Xanthomonas oryzae pv. oryzae. Physiol Mol Plant Pathol, 2003, 62: 203–208
[17]Smith S M, Hulbert S H. Recombination events generating a novel Rp1 race specificity. Mol Plant Microbe Interact, 2005, 18: 220–228
[18]Lawrence G J, Finnegan E J, Ayliffe M A, Ellis J G. The l6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. Plant Cell, 1995, 7: 1195–1206
[19]Stewart H E, Bradshaw J E, Pande B. The effect of the presence of R-genes for resistance to late blight (Phytophthora infestans) of potato (Solanum tuberosum) on the underlying level of field resistance. Plant Pathol, 2003, 52: 193–198
[20]Parniske M, Hammond-Kosack K E, Golstein C, Thomas C M, Jones D A, Harrison K, Wulff B B, Jones J D. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato. Cell, 1997, 91: 821–832
[21]Whitaker V M, Zuzek K, Hokanson S C. Resistance of 12 rose genotypes to 14 isolates of Diplocarpon rosae Wolf (rose blackspot) collected from eastern North America. Plant Breed, 2007, 126: 83–88
[22]Li Z K, Arif M, Zhong D B, Fu B Y, Xu J L, Domingo-Rey J, Ali J, Vijayakumar C H, Yu S B, Khush G S. Complex genetic networks underlying the defensive system of rice (Oryza sativa L.) to Xanthomonas oryzae pv. oryzae. Proc Natl Acad Sci USA, 2006, 103: 7994–7999
[23]Parlevliet J E. Race-specific aspects of polygenic resistance of barley to leaf rust, Puccinia hordei. Eur J Plant Pathol, 1978, 84: 121–126
[24]Perchepied L, Dogimont C, Pitrat M. Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon. Theor Appl Genet, 2005, 111: 65–74
[25]Darvishzadeh R, Poormohammad Kiani S, Dechamp-Guillaume G, Gentzbittel L, Sarrafi A. Quantitative trait loci associated with isolate specific and isolate nonspecific partial resistance to Phoma macdonaldii in sunflower. Plant Pathol, 2007, 56: 855–861
[26]Arru L, Francia E, Pecchioni N. Isolate-specific QTLs of resistance to leaf stripe (Pyrenophora graminea) in the ‘Steptoe’ × ‘Morex’ spring barley cross. Theor Appl Genet, 2003, 106: 668–675
[27]Wang Z-L(王竹林), Liu S-D(刘曙东), Wang H(王辉), He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民), Duan X-Y(段霞瑜), Zhou Y-L(周益林). Advances of study on adult-plant resistance in bread wheat. J Triticeae Crops (麦类作物学报), 2006, 26(1): 129–134 (in Chinese with English abstract)
[28]Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder M S, Weber W E. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet, 2002, 105: 921–936
[29]Huo N-X(霍纳新), Zhou R-H(周荣华), Zhang L-F(张丽芳), Jia J-Z(贾继增). Mapping quantitative trait loci for powdery mildew resistance in wheat. Acta Agron Sin (作物学报), 2005, 31(6): 692–696 (in Chinese with English abstract)
[30]Sheng B-Q(盛宝钦). Investigation of host-plant response to Blumeria graminis f. sp. tritici at seedling stage in wheat. Plant Protect (植物保护), 1988, (1): 49 (in Chinese with English abstract)
[1] ZHANG Bo, PEI Rui-Qing, YANG Wei-Feng, ZHU Hai-Tao, LIU Gui-Fu, ZHANG Gui-Quan, WANG Shao-Kui. Mapping and identification QTLs controlling grain size in rice (Oryza sativa L.) by using single segment substitution lines derived from IAPAR9 [J]. Acta Agronomica Sinica, 2021, 47(8): 1472-1480.
[2] ZHOU Xin-Tong, GUO Qing-Qing, CHEN Xue, LI Jia-Na, WANG Rui. Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(4): 587-598.
[3] LI Shu-Yu, HUANG Yang, XIONG Jie, DING Ge, CHEN Lun-Lin, SONG Lai-Qiang. QTL mapping and candidate genes screening of earliness traits in Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(4): 626-637.
[4] SHEN Wen-Qiang, ZHAO Bing-Bing, YU Guo-Ling, LI Feng-Fei, ZHU Xiao-Yan, MA Fu-Ying, LI Yun-Feng, HE Guang-Hua, ZHAO Fang-Ming. Identification of an excellent rice chromosome segment substitution line Z746 and QTL mapping and verification of important agronomic traits [J]. Acta Agronomica Sinica, 2021, 47(3): 451-461.
[5] Dai-Ling LIU,Jun-Feng XIE,Qian-Rui HE,Si-Wei CHEN,Yue HU,Jia ZHOU,Yue-Hui SHE,Wei-Guo LIU,Wen-Yu YANG,Xiao-Ling WU. QTL analysis for relative contents of glycinin and β-conglycinin fractions from storage protein in soybean seeds under monoculture and relay intercropping [J]. Acta Agronomica Sinica, 2020, 46(3): 341-353.
[6] WU Hai-Tao, ZHANG Yong, SU Bo-Hong, Lamlom F Sobhi, QIU Li-Juan. Development of molecular markers and fine mapping of qBN-18 locus related to branch number in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2020, 46(11): 1667-1677.
[7] WANG Cun-Hu,LIU Dong,XU Rui-Neng,YANG Yong-Qing,LIAO Hong. Mapping of QTLs for leafstalk angle in soybean [J]. Acta Agronomica Sinica, 2020, 46(01): 9-19.
[8] YANG Xiao-Meng, LI Xia, PU Xiao-Ying, DU Juan, Muhammad Kazim Ali, YANG Jia-Zhen, ZENG Ya-Wen, YANG Tao. QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population [J]. Acta Agronomica Sinica, 2020, 46(01): 52-61.
[9] WANG Da-Chuan,WANG Hui,MA Fu-Ying,DU Jie,ZHANG Jia-Yu,XU Guang-Yi,HE Guang-Hua,LI Yun-Feng,LING Ying-Hua,ZHAO Fang-Ming. Identification of rice chromosome segment substitution Line Z747 with increased grain number and QTL mapping for related traits [J]. Acta Agronomica Sinica, 2020, 46(01): 140-146.
[10] Li-Juan WEI,Rui-Ying LIU,Li ZHANG,Zhi-You CHEN,Hong YANG,Qiang HUO,Jia-Na LI. Detection of stem height QTL and integration of the loci for plant height- related traits in B. napus [J]. Acta Agronomica Sinica, 2019, 45(6): 818-828.
[11] YAN Chao,ZHENG Jian,DUAN Wen-Jing,NAN Wen-Bin,QIN Xiao-Jian,ZHANG Han-Ma,LIANG Yong-Shu. Locating QTL controlling yield traits in overwintering cultivated rice [J]. Acta Agronomica Sinica, 2019, 45(4): 522-537.
[12] ZHANG Chun-Xiao,LI Shu-Fang,JIN Feng-Xue,LIU Wen-Ping,LI Wan-Jun,LIU Jie,LI Xiao-Hui. QTL mapping of salt and alkaline tolerance-related traits at the germination and seedling stage in maize (Zea mays L.) using three analytical methods [J]. Acta Agronomica Sinica, 2019, 45(4): 508-521.
[13] LIU Jiang-Ning,WANG Chu-Xin,ZHANG Hong-GEN,MIAO Yi-Xu,GAO Hai-Lin,XU Zuo-Peng,LIU Qiao-Quan,TANG Shu-Zhu. Mapping of QTLs for resistance to rice black-streaked dwarf disease [J]. Acta Agronomica Sinica, 2019, 45(11): 1664-1671.
[14] Wen-Xiang WANG,Wen CHU,De-Sheng MEI,Hong-Tao CHENG,Lin-Lin ZHU,Li FU,Qiong HU,Jia LIU. Quantitative trait loci mapping for branch angle and candidate gene screening in Brassica napus L. [J]. Acta Agronomica Sinica, 2019, 45(1): 37-45.
[15] Chao LI,Zhi-Kun LI,Qi-Shen GU,Jun YANG,Hui-Feng KE,Li-Qiang WU,Guo-Ning WANG,Yan ZHANG,Jin-Hua WU,Gui-Yin ZHANG,Yuan-Yuan YAN,Zhi-Ying MA,Xing-Fen WANG. Molecular Evaluation for Chromosome Segment Substitution Lines of Gossypium barbadense and QTL Mapping for Fiber Quality and Yield [J]. Acta Agronomica Sinica, 2018, 44(8): 1114-1126.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd