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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 23-35.doi: 10.3724/SP.J.1006.2012.00023


Characterizations of a Mutant Gene hw-1(t) for Green-revertible Albino, High Tillering and Dwarf in Rice (Oryza sativa L.)

GUO Tao**,HUANG Xuan**,HUANG Yong-Xiang,LIU Yong-Zhu,ZHANG Jian-Guo,CHEN Zhi-Qiang*,WANG Hui*   

  1. South China Agricultural University / National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2011-05-04 Revised:2011-09-12 Online:2012-01-12 Published:2011-11-07
  • Contact: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

Abstract: A rice (Oryza sativa L.) mutant hfa-1 exhibiting green-revertible albino, high-tillering dwarf was detected from a M2 population of an American variety Francis by space mutagenesis. hfa-1 displayed distinctive albino before 3rd leaf stage but finally turned to normal green after 3rd leaf stage, and the expression of this phenotype was conditionally controlled by development and temperature. Examining ultrastructure and measuring chlorophyll content indicated that hfa-1 showed albino due to abnormal development of chloroplast, which resulted in inhibiting chlorophyll synthesis and decreasing photosynthetic efficiency. Moreover, the increased tiller number of hfa-1 was ascribed to initiate more higher-order tiller buds. Further endogenous hormones analysis demonstrated that the enhanced tillering capacity of hfa-1 might not result from inhibiting the synthesis of IAA. In addition, the dwarfism of hfa-1 was caused by shortening internodes and was independent of biosynthesis and signal transduction of GA. Genetic analysis indicated that the phenotype of green-revertible albino, high-tillering dwarf in hfa-1 was controlled by a recessive nucleic gene, namely hw-1(t). Using a large F2 mapping population derived from a cross between hfa-1 and an japonica rice variety, 02428, hw-1(t) was fine mapped into a 46.9 kb of physical distance between two InDel markers, HW27 and HW7 on chromosome 4, where 13 open reading frames were predicted. In the mapping interval LOC_Os04g57320 encoded a IMMUTANTS protein, which was the most properly candidate gene of hw-1(t).

Key words: Rice (Oryza sativa L.), Green-revertible albino, High-tillering dwarf, hw-1(t), Fine mapping

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