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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 285-292.doi: 10.3724/SP.J.1006.2012.00285


High Light Tolerance is Enhanced by Overexpressed PEPC in Rice under Drought Stress

DING Zai-Song,ZHOU Bao-Yuan,SUN Xue-Fang,ZHAO Ming*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Cultivition, Ministry of Agriculture, Beijing 100081, China
  • Received:2011-04-12 Revised:2011-09-29 Online:2012-02-12 Published:2011-12-01
  • Contact: 赵明, E-mail: zhaoming@caas.net.cn, Tel: 01082108752

Abstract: Overexpression of phosphoenolpyruvate carboxylase (PEPC) gene in transgenic rice to improve photosynthesis and stress resistance have brought extensive interest in the world. Our previous report proved that the overexpressed PEPC alleviate drought stress inhibition on photosynthesis. In the present research, photosynthetic light curve, chlorophyll a fluorescence parameters, pigment content and reactive oxygen metabolism were studied under drought stress at flowering stage in 2 lines of PEPC transgenic rice plants. The results showed that under drought especially severe drought stress, net photosynthetic rate decreased dramatically under high photosynthetic active radiation (PAR) in wild type untransformed rice, while maintained unchanged in PEPC transgenic lines under high PAR even more than 1 200 μmol m-2 s-1. And the photochemistry activities (Fv/Fm, ΦPSⅡ, qP) decreased less under drought stress in both the PEPC transgenic lines. These indicated PEPC enhanced the photoinhibition tolerance of rice under drought stress. The higher zeaxanthin content under drought stress made the PEPC transgenic rice leaves disperse more light energy as heat, thus decreased the O2? producing rate in PSII. At the same time, the SOD, POD and CAT activities were higher in PEPC transgenic rice plants under drought stress, they could effectively diminished the reactive oxygen species and reduced the membrane lipid peroxidation.

Key words: PEPC transgenic rice, Drought, Photoinhibition, Chlorophyll fluorescence parameter, Reactive oxygen metabolism

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