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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 865-870.doi: 10.3724/SP.J.1006.2012.00865


Accumulation and Distribution of Hydrogen Peroxide in Interaction between Sugarbeet Plant and Sugarbeet Necrotic Yellow Vein Virus

CHEN Yu-Zhen1,ZHANG Shao-Ying1,*,KANG Zhen-Sheng2,HAN Qing-Mei2,BAI Zhen-Qing1

  1. 1 College of Agronomy, Inner Mongolia Agricultural University / Sugarbeet Physiological Institute, Huhhot 010019, China; 2 Northwest Sci-Tech University of Agriculture and Forestry / State Key Laboratory of Crop Stress Biology in the Arid Areas, Yangling 712100, China
  • Received:2011-11-03 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张少英, E-mail: syzh36@yahoo.com.cn, Tel: 0471-4301709 E-mail:chenyuzhen818@126.com

Abstract: Hydrogen peroxide (H2O2) plays an important role in the interaction between plant and pathogen defense response. The objective of thisresearch was owing the interaction system of beet necrotic yellow vein virus (BNYVV) and resistant, susceptible sugarbeet lines to disclose the relationship of accumulation and distribution of H2O2 with sugarbeet rhizomania resistance. The production and accumulation of H2O2 in the two interaction systems were detected and the distribution of H2O2 was observed in subcellular level using the cytochemical technique. The result showed that there was plenty of H2O2 in both interaction systems, and the production of H2O2 was evidently higher in the resistant lines than with in the susceptible lines. In addition, distribution and the location of H2O2 were similar in the two lines, where we observed mainly in the tonoplast and the plasma membrane of host root, vein cells as well as the intercellular space of some host vein cells. But there were obvious differences in the content of H2O2 accumulated in the two interaction lines, the deposition of H2O2 in the susceptible line was significantly lower than that in the resistant line, showing it may have a close relationship with resistance of sugarbeet against rhizomania, H2O2 showed the involved in the defense responses to virus infection in sugar beets.

Key words: Sugarbeet, BNYVV, H2O2, Cytochemistry

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