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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 863-870.doi: 10.3724/SP.J.1006.2010.00863

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Mechanism of Tolerance to Drought in Sugarcane Plant Enhanced by Foliage Dressing of Abscisic Acid under Water Stress

LI Chang-Ning1,2,Manoj Kumar SRIVASTAVA2,NONG Qian1,LI Yang-Rui2,*   

  1. 1College of Agriculture,Guangxi University,Nanning 530004,China;2Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Nanning 530007,China
  • Received:2009-10-20 Revised:2010-02-05 Online:2010-05-12 Published:2010-03-15
  • Contact: LI Yang-Rui，E-mail:lyr@gxaas.net E-mail:lcn560@163.com

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Abstract:

Drought is a major limiting factor for sugarcane growth. Abscisic acid (ABA) regulates much important plant physiological and biochemical processes, and induces tolerance to different stresses including drought. Understanding the mechanism of tolerance to drought in sugarcane plant with foliage dressing of ABA under water stress would facilitate breeding and field management for improving drought resistance. Thus, an experiment was set up to investigate the interrelationship between drought induced ABA biosynthesis and antioxidative defense system, and to confer the farther role of foliar application of ABA in imparting drought tolerance to the sugarcane plant. The treatments were: T1 (drought), T2 (drought + foliar application of 15 µmol L–1 ABA) and C (control, normal irrigation) using a drought tolerant cultivar ROC22. The results showed that drought treatment (T1) enhanced the ABA concentration in leaf that was significantly higher in combined treatment (T2), suggesting the ABA biosynthesis was triggered in leaf by the ABA application. Both T1 and T2 resulted in an increase in proline, H2O2 and MDA contents while the exogenous ABA alleviated the increase in MDA content. ABA application decreased the degradation of chlorophyll, and counteracted, at least in part, the decrease in maximal PSII efficiency (Fv/Fm) and quantum efficiency of PSII (ΦPSII). Overproduction of H2O2 in T1 was followed by increasing activities of CAT, GPX, GR, and APX, which was further improved by the ABA treatment (T2). A decrease in H2O2 level with increasing stress in T2 showed that ABA highly induced antioxidative defense system which was found to be vanished progressively in T1. The results clearly suggest that the tolerant cultivar showed an enhanced protective system against drought conditions, and the foliar application of ABA further improved its tolerance by triggering the over expression of antioxidative defense system.

Key words: ABA, Sugarcane, Drought tolerance, Antioxidative enzyme, H2O2, Chlorophyll fluorescence


[1] Duan B L, Yang Y Q, Lu Y W, Helena K, Frank B, Li C Y. Interactions between water deficits, ABA, and provenances in Picea asperata. J Exp Bot, 2007, 58: 3025-3036

[2] Jiang M Y, Zhang J H. Abscisic acid and antioxidant defense in plant Cells. Acta Bot Sin, 2004, 46: 1-9

[3] Ikegami K, Okamoto M, Seo M, Koshiba T. Activation of abscisic acid biosynthesis in the leaves of Arabidopsis thaliana in response to water deficit. J Plant Res, 2009, 122: 235-243

[4] Zhang J H, Jia W S, Yang J C, Ismail A M. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Res, 2006, 97: 111-119

[5] Jiang M Y, Zhang J H. Role of abscisic acid in water stress induced antioxidant defence in leaves of maize seedlings. Free Radical Res, 2002, 36: 1001-1015

[6] Jiang M Y, Zhang J H. Water stress induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot, 2002, 53: 2401-2410

[7] Jiang M Y, Zhang J H. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol, 2001, 42: 1265-1273

[8] Murata Y, Pei Z M, Mori I C, Schroeder J L. Abscisic acid activation of plasma membrane Ca2+ channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants. Plant Cell, 2001, 13: 2513-2523

[9] Zhang X, Zhang L, Dong F C, Gao J F, Galbraith D W, Song C P. Hydrogen peroxide is involved in abscisic acid induced stomatal closure in Vicia faba. Plant Physiol, 2001, 126: 1438-1448

[10] Anderson M D, Prasad T K, Martin B A, Stewart C R. Differential gene expression in chilling acclimated maize seedlings and evidence for the involvement of abscisic acid in chilling tolerance. Plant Physiol, 1994, 105: 331-339

[11] Guan L Q, Scandalios J G. Two structurally similar maize cytosolic superoxide dismutase genes, Sod 4 and Sod4A, respond differentially to abscisic acid and high osmoticum. Plant Physiol, 1998, 117: 217-224

[12] Kamminaka H, Morita S, Tokumoto M, Masumura T, Tanaka K. Differential gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radical Res, 1999, 31: 219-225

[13] Shao H B, Liang Z S, Shao M A, Wang B C. Changes of anti-oxidative enzymes and membrane peroxidation for soil water deficits among 10 wheat genotypes at seedling stage. Colloids Surfaces, 2005, 42: 107-113

[14] Wang W(王玮), Zhang F(张枫), Li D-Q(李德全). The effects of exogenous ABA on osmotic adjustment in maize roots under osmotic stress. Acta Agron Sin (作物学报), 2002, 28(1): 121-126 (in Chinese with English abstract)

[15] Agarwal S, Sairam R K, Srivastava G C, Meena R C. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biol Plant, 2005, 49: 541-550

[16] Li C Y, Yin C Y, Liu S R. Different responses of two contrasting Populus davidiana populations to exogenous abscisic acid application. Environ Exp Bot, 2004, 51: 237-246

[17] Gong M, Li Y J, Chen S Z. Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems. J Plant Physiol, 1998, 153: 488-496

[18] Hu XCalcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. New Phytol, 2007, 173: 27-38, Jiang M, Zhang J, Zhang A, Lin F, Tan M.

[19] Health R L, Packer L. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives Biochem Biophysics, 1968, 125: 189-198

[20] Bates L S, Waldren R P, Teare I D. Rapid determination of free proline for water stress studies. Plant Soil, 1973, 39: 205-207

[21] Brennan T, Frenkel C. Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol, 1977, 59: 411-416

[22] Aebi H. Catalase in vitro. Methods Enzymol, 1984, 105: 121-126

[23] Schaedle M, Bassham J A. Chloroplast glutathione reductase. Plant Physiol, 1977, 59: 1011-1012.

[24] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol, 1981, 22: 867-880

[25] Zheng X, Van Huystee R B. Peroxidase regulated elongation of segments from peanut hypocotyls. Plant Sci, 1992, 81: 47-56

[26] Liu J, Jiang M Y, Zhou Y F, Liu Y L. Production of polyamines is enhanced by endogenous abscisic acid in maize seedlings subjected to salt stress. J Integr Plant Biol, 2005, 47: 1326-1334

[27] Gomez C A, Arbona V, Jacas J, Primo M E, Talon M. Abscisic acid reduces leaf abscission and increases salt tolerance in citrus plants. J Plant Growth Regul, 2003, 21: 234-240

[28] Iqbal M, Ashraf M, Rehman S, Rha E S. Does polyamine seed pretreatment modulate growth and levels of some plant growth regulators in hexaploid wheat (Triticum aestivum L.) plants under salt stress? Bot Studies, 2006, 47: 239-250

[29] Smirnoff N, Cumbes Q J. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry, 1989, 28: 1057-1060

[30] Bassi R, Rigoni F, Giacometti G M. Chlorophyll binding proteins with antenna function in higher plants and green algae. Photochem Photobiol, 1990, 52: 1187-1206

[31] Kause G H, Weis E. Chlorophyll fluorescence and photosynthesis: The basis. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42: 313-349

[32] Jiang M Y, Zhang J H. Involvement of plasma membrane NADPH oxidase in abscisic acid and water stress-induced antioxidant defense in leaves of maize seedling. Planta, 2002, 215: 1022-1030

[33] Shinozaki K, Yamaguchi S K. Gene expression and signal transduction in water stress response. Plant Physiol, 1997, 115: 327-334
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