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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 962-970.doi: 10.3724/SP.J.1006.2012.00962


Exploring Elite Alleles for Chlorophyll Content of Flag Leaf in Natural Population of Wheat by Association Analysis

LI Wei-Yu1,2,ZHANG Bin2,ZHANG Jia-Nan2,3,CHANG Xiao-Ping2, LI Run-Zhi1,*,JING Rui-Lian2,*   

  1. 1 Agronomy College, Shanxi Agricultural University, Taigu 030801, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 National Millet Improvement Center of China, Institute of Millet Crops / Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
  • Received:2012-01-19 Revised:2012-04-18 Online:2012-06-12 Published:2012-04-23
  • Contact: 景蕊莲, E-mail: jingrl@caas.net.cn, Tel: 010-82105829; 李润植, E-mail: rli2001@hotmail.com

Abstract: Elite alleles associated with chlorophyll content of flag leaf in a natural population of winter wheat (Triticum aestivum L.) consisting of 262 accessions were measured at flowering and grain-filling stages under both rainfed and well-watered conditions in two experimental locations in Beijing, China. A total of 169 SSR markers distributed on the 21 chromosomes of wheat were employed to detect the genetic diversity and genetic structure of the population. Association analysis between SSR loci and chlorophyll content trait was performed using TASSEL MLM (mixed linear model) program. The phenotypic allele effect was estimated through comparing the average phenotypic value over accessions with that of “null allele”. A total of 2048 alleles were identified on the 169 SSR loci, and each locus had 2–37 alleles with an average of 12. The polymorphism information contents (PICs) of the SSR loci ranged from 0.008 to 0.936 with an average of 0.628. Forty markers were found to be significantly associated with chlorophyll content (P<0.001) on 22 loci, of which 11 markers showed repeated associations, particularly, loci Xwmc419-1B and Xgwm501-2B had associations for three times. The alleles on loci Xcfa2123-7A, Xgwm232-1D, and Xgwm429-2B exhibited more than 4.0 of phenotypic effect value.

Key words: Wheat (Triticum aestivum L.), Chlorophyll content, SSR, Association analysis, Allelic variation

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