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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 971-979.doi: 10.3724/SP.J.1006.2012.00971

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation of GsGST19 from Glycine soja and Analysis of Saline-Alkaline Tolerance for Transgenic Medicago sativa

WANG Zhen-Yu1,2,3,CAI Hua1,BAI Xi1,JI Wei1,LI Yong1,WEI Zheng-Wei1,ZHU Yan-Ming1,*   

  1. 1 Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030, China; 2 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, China; 3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-11-17 Revised:2012-02-22 Online:2012-06-12 Published:2012-04-06
  • Contact: 朱延明, E-mail: ymzhu2001@neau.edu.cn E-mail:wangzhenyu128@163.com

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Abstract: Alfalfa (Medicago sativa L.) is one of the most important leguminous forage crops worldwide. Saline-alkaline stress significantly limits the productivity of alfalfa due to its adverse effects on growth, formation of nodules, and symbiotic nitrogen-fixation capacity, and resulting in the formation of reactive oxygen species (ROS). To protectplants from the toxicity ofreactiveoxygen, aerobic organisms are equipped with an array of defense mechanisms, including one based on glutathione S-transferases (GSTs). Glutathione S-transferases (GSTs) are ubiquitous enzymes that play a key role in stress tolerance and cellular detoxification. The GST gene GsGST19 isolated fromwild type soybean(Glycine soja) under saline-alkaline stress was transformed into alfalfa (Medicago sativa L.). Transgenic alfalfa plants showed 0.52–0.49 times higher levels of GST activity than wild type plants. Transgenic alfalfa grew well in the conditions of 100 mmol L–1 NaHCO3, while wild type plants exhibited discoloration and stunted growth, or even death. There were significantly changes in malondialdehyde content and relative membrane permeability caused by saline-alkaline stress in non-transgenic lines compared to transgenic lines (P<0.05). Moreover, compared with non-transgenic, transgenic alfalfa had higher levels of chlorophyll content and root activity under saline-alkali stress conditions. The results indicated that the gene GsGST19 could enhanceresistance to saline-alkaline in alfalfa.

Key words: Glutathione S-transferase, Transgenic alfalfa, Glycine soja, Saline-alkaline stress

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