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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1351-1359.doi: 10.3724/SP.J.1006.2011.01351

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Tolerance Analysis of GsNAC20 Gene Linked to Response to Stress in Glycine soja

CAI Hua,ZHU Yan-Ming*,LI Yong,BAI Xi,JI Wei,WANG Dong-Dong,SUN Xiao-Li   

  1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China,
  • Received:2011-01-16 Revised:2011-04-27 Online:2011-08-12 Published:2011-06-13
  • Contact: 朱延明,E-mail:ymzhu2001@yahoo.com.cn

Abstract: Abiotic stresses, such as salt and drought, affect plant growth, development and reduce crop yield. Isolationof a key regulatory gene linked to response to abiotic-stress and identification of thegenes function areurgently needed. Glycine soja is an excellent material to isolate abiotic stress-related genes because of its high stress tolerance. Plant-specific transcription factor NAC (NAM, ATAF1/2, CUC2) proteins play essential roles in many biological processes such as development, senescence, morphogenesis, and stress signal transduction pathways. It has become a new research focus in the abiotic-stress field. Based on that, we screened a new NAC gene from Glycine soja by yeast one hybrid, which has 99% similarity with NAC20 of Glycine max (EU440353.1), named as GsNAC20. GsNAC20 had typical NAC DNA-binding domain at the N-terminal and transcription activation region at the C-terminal. It can bind to MYB1AT element (the core sequence: AAACCA) in vitro, but no transcriptional activation activity in the yeast assay system, which was consistent with GmNAC20. Localization of GsNAC20 protein was analyzed by transient expression in tobacco epidermis cells and the result showed that GsNAC20 waslocalized in nucleus. Semi-quantitative RT-PCR showed the expression level of GsNAC20 was induced by drought, low temperature and salt stresses, but there existed difference between leaf and root in G. soja. Arabidopsis thaliana plants overexpressing GsNAC20 showed higher sensitivity under salt stress. All results showed that GsNAC20 perhaps is a new member of NAC family in G. soja, and is closely related to salt and drought stresses, so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance in plant.

Key words: Glycine soja, Transcription factor, GsNAC20, Abiotic stress

[1]Li P(李鹏), Huang G-Q(黄耿青), Li X-B(李学宝). Plant NAC transcription factors. Plant Physiol Mol Biol (植物生理学通讯), 2010, 46(3): 294–300 (in Chinese with English abstract)
[2]Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell, 1997, 9: 841–857
[3]Hu H H, Dai M Q, Yao J L, Xiao B Z, Li X H, Zhang Q F, Xiong L Z. Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA, 2006, 103: 12987–12992
[4]Zheng X, Chen B, Lu G, Han B. Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochem Biophys Res Commun, 2009, 379: 985–989
[5]Lu P L, Chen N Z, An R, Su Z, Qi B S, Ren F, Chen J, Wang X C. A novel drought-inducible gene, ATAF1, encodes a NAC family protein that negatively regulates the expression of stress-responsive genes in Arabidopsis. Plant Mol Biol, 2007, 63: 289−305
[6]Tran L S, Nakashima K, Sakuma Y, Simpson S D, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell, 2004, 16: 2481–2498
[7]Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran L S, Yamaguchi-Shinozaki K, Shinozaki K. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J, 2004, 39: 863–876
[8]Souer E, Houwelingen V A, Kloos D, Mol J, Koes R. The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordial boundaries. Cell, 1996, 85: 159–170
[9]Riechmann J L, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, RatcliVe O J, Samaha R R, Creelman R, Pilgrim M, Broun P, Zhang J Z, Ghandehari D, Sherman B K, Yu G L. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000, 290: 2105–2110
[10]Jensen M K, Rung J H, Gregersen P L. The HvNAC6 transcription factor: a positive regulator of penetration resistance in barley and Arabidopsis. Plant Mol Biol, 2007, 65: 137–150
[11]Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res, 2003, 10: 239–247
[12]Fang Y, You J, Xie K, Xie W, Xiong L. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Genet Genomics, 2008, 280: 535–546
[13]Liu Z-J(柳展基), Shao F-X(邵凤霞), Tang G-Y(唐桂英), Shan L(单雷), Bi Y-P(毕玉平). Cloning and characterization of a transcription factor ZmNAC1 in maize (Zea mays). Hereditas (遗传), 2009, 31(2): 199–205 (in Chinese with English abstract)
[14]Liu X(刘旭), Li L(李玲). Cloning and characterization of the NAC-like gene AhNAC2 and AhNAC3 in peanut. Acta Agron Sin (作物学报), 2009, 35(3): 541–545 (in Chinese with English abstract)
[15]Meng Q C, Zhang C H, Gai J Y, Yu D Y. Molecular cloning, sequence characterizatiion and tissue specific expression of six NAC-likegenes in soybean (Glycine max L. Merr.). J Plant Physilol, 2007, 164: 1002–1012
[16]Abe H, Urao T, Ito T, Seki M, Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15: 63–78
[17]Wang X(王希), Li Y(李勇), Zhu Y-M(朱延明), Bai X(柏锡), Cai H(才华), Ji W(纪巍). Cloning and tolerance analysis of GsANN gene related to response on stressin in Glycine soja. Acta Agron Sin (作物学报), 2010, 36(10): 1666–1673 (in Chinese with English abstract)
[18]Olsen A N, Ernst H A, Leggio L L, Skriver K. NAC transcription factors: structurally distinct, functionally diverse. Trends Plant Sci, 2005, 10: 79–87
[19]Hao Y J, Song Q X, Chen H W, Zou H F, Wei W, Kang X S, Ma B, Zhang W K, Zhang J S, Chen S Y. Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation. Planta, 2010, 232: 1033–1043
[20]Tran L S, Quach T N, Guttikonda S K, Aldrich D L, Kumar R, Neelakandan A, Valliyodan B, Nguyen H T. Molecular characterization of stress-inducible GmNAC genes in soybean. Mol Genet Genomics, 2009, 281: 647–664
[21]Wu Y R, Deng Z Y, Lai J B, Zhang Y Y, Yang C P, Yin B J, Zhao Q Z, Zhang L, Li Y, Yang C W, Xie Q. Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses. Cell Res, 2009, 19: 1279–1290
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