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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1360-1371.doi: 10.3724/SP.J.1006.2011.01360


Postulation of Stripe Rust Resistance Genes and Analysis of Adult Resistance in 50 Wheat Varieties (Lines) in Gansu Province

CAO Shi-Qin1,2,ZHANG Bo2,LI Ming-Ju3,4,XU Shi-Chang3,LUO Hui-Sheng2,JIN She-Lin2,*,JIA Qiu-Zhen2,HUANG Jin2,SHANG Xun-Wu1   

  1. 1College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 2Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 4 Institute of Agriculture Environment and Resource, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
  • Received:2011-01-19 Revised:2011-04-27 Online:2011-08-12 Published:2011-06-13
  • Contact: 金社林, E-mail: jinshelin@163.com; 尚勋武, E-mail: shangxuwu@163.com

Abstract: By means of artificial inoculation of 26 Puccinia striiformis isolates with different spectra of pathogenicity at seedling stage, the resistance to strip rust was identified in 50 wheat varieties (lines) from Gansu province, China. In combination with pedigree analysis and resistance reactions of 30 standard varieties with known resistant genes, the resistance genes in the 50 varieties (lines) were postulated. The resistant genes Yr5, Yr10, Yr15, Yr26, and Yr9 (1B/1R) were validated using molecular markers in 43 varieties (lines). The results of resistance gene postulation showed that Zhongliang 25 was carrying Yr3 and unknown genes. The vareity Lantian 20 was postulated to possess Yr3a, Yr4a, Yr16, and unknown genes. The line Y9220-12 was postulated to possess Yr9, YrCle, and unknown genes. The varieties (lines) Lantian 14, Longyuan 932, Longyu 216, and Longyuan 992 were postulated to possess Yr9 and unknown genes. Genes Yr10 and YrMor were postulated in Longjian 9343, 93-Bao 4-4, Tianxuan 43, and Guinong 22. The variety Lantian 19 was postulated to possess Yr12 and unknown resistant genes. Gene Yr26 was postulated in Lantian 17, 95-111-3, 98-178-3-2-4, and 92R137. Fourteen varieties (lines) were detected to be Yr9 (1B/1R) wheat–rye translocation, which accounted for 32.6% of the total varieties. Yr26 was detected in 92R178 and Lantian 17. Other varieties (lines) were controlled by unknown genes. Although most varieties (lines) tested were highly susceptible at seedling stage, some of them showed adult resistance in the observation pool in Longnan, Gansu, China. Ten varieties (lines) including Lantian 10 might possess adult resistance and another 10 varieties (lines) including Lantian 14 might be varieties with slow rusting characters.

Key words: Wheat stripe rust, Resistance gene, Gene postulation, Molecular detection, Gansu Province of China

[1]Li Z-Q(李振岐), Zeng S-M(曾士迈). Wheat Rust in China (中国小麦锈病). Beijing: China Agriculture Press, 2002 (in Chinese)
[2]Chen W-Q(陈万权), Wang J-X(王剑雄). Genes for leaf and stem rust resistance in 76 wheat genetic resources. Acta Agron Sin (作物学报), 1997, 23(6): 655-663 (in Chinese with English abstract)
[3]Niu Y-C(牛永春), Qiao Q(乔奇), Wu L-R(吴立人). Postulation of resistance genes to stripe rust in commercial wheat cultivars from Henan, Shandong and Anhui provinces. Acta Phytopathol Sin (植物病理学报), 2000, 30(2): 122-128 (in Chinese with English abstract)
[4]Wang F-L(王凤乐), Wu L-R(吴立人), Wan A-M(万安民), Song W-Z(宋位中). Analysis of resistance genes to stripe rust in commercial wheat cultivars from Shanxi, Gansu and Sichuan provinces. Acta Agron Sin (作物学报), 1994, 20(5): 589-594 (in Chinese with English abstract)
[5]Wang F-L(王凤乐), Wu L-R(吴立人), Xie S-X(谢水仙), Wan A-M(万安民). Postulation of genes and adult resistance to stripe rust of Chinese important wheat resistance sources. Acta Phytopathol Sin (植物病理学报), 1994, 24(2): 175-180 (in Chinese with English abstract)
[6]Chen W-Q(陈万权), Qin Q-M(秦庆明), Chen Y-L(陈扬林), Wu Z-X(吴支行), Ma Z-Q(马志强), Liao Q(廖琴). Leaf rust resistance of 40 wheat cultivars in China. Acta Phytopathol Sin (植物病理学报), 2001, 31(1): 16-25 (in Chinese with English abstract)
[7]Chen W-Q(陈万权), Hu C-C(胡长程). Postulation of genes for leaf rust resistance in 28 Chinese wheat. Acta Agron Sin (作物学报), 1993, 19(3): 268-275 (in Chinese with English abstract)
[8]Chen W-Q(陈万权), Qin Q-M(秦庆明), Wang K-R(王奎荣), Xia S-B(夏善保), Feng G-H(冯国华), Chen R-Z(陈荣振). Genes for leaf and stem rust resistance in important wheat cultivars in Jiangsu province. Acta Phytophylacica Sin (植物保护学报), 1997, 24(3): 225-233 (in Chinese with English abstract)
[9]Chen W-Q(陈万权), Wang J-X(王剑雄). Genes for leaf and stem rust resistance in 76 wheat genetic resources. Acta Agron Sin (作物学报), 1997, 23(6): 655-663 (in Chinese with English abstract)
[10]Yuan J-H(袁军海), Liu T-G(刘太国), Chen W-Q(陈万权). Postulation of leaf rust resistance genes in 47 new wheat cultivars (lines) at seedling stage. Sci Agric Sin(中国农业科学), 2007, 40(9): 1925-1935 (in Chinese with English abstract)
[11]Li Z F, Xia X C, Zhou X C, Niu Y C, He Z H, Zhang Y, Li G Q, Wan A M, Wang D S, Chen X M, Lu Q L, Singh R P. Seedling and slow rusting resistances to stripe rust in Chinese common wheat. Plant Dis, 2006, 90: 1302-1312
[12]Francis H A, Leitch A R, Koebner R M D. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theor Appl Genet, 1995, 90: 636-642
[13]Chen X M, Soria M A, Yan G P, Sun J, Dubcovsky J. Development of sequence tagged site and cleaved amplified polymorphic sequence markers for wheat stripe rust resistance gene Yr5. Crop Sci, 2003, 43: 2058-2064
[14]Shao Y-T(邵映田), Niu Y-C(牛永春), Zhu L-H(朱立煌), Zhai W-X(翟文学), Xu S-C(徐世昌), Wu L-R(吴立人). Identification of all AFLP marker linked to the stripe rust resistance gene Yr10 in wheat. Chin Sci Bull (科学通报), 2001, 46(8): 669-672 (in Chinese with English abstract)
[15]Peng J H, Fahima T, Roeder M S, Huang Q Y, Dahan A, Li Y C, Grama A, Nevo E. High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yrl5 derived from wild emmer wheat, Triticum dicoccoides. Genetica, 2000, 109: 199-210
[16]Ma J X, Zhou R H, Dong Y S, Wang L F, Wang X M, Jia J Z.Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica, 2001, 120: 219-226
[17]Wang C M, Zhang Y P, Han D J, Kang Z S, Li G P, Cao A Z, Chen P D. SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica, 2008, 159: 359-366
[18]Li F-Q(李峰奇), Han D-J(韩德俊), Wei G-R(魏国荣), Zeng Q-D(曾庆东), Huang L-L(黄丽丽), Kang Z-S(康振生). Molecular detection of stripe rust resistant genes in 126 winter wheat varieties from the Huanghuai wheat region. Sci Agric Sin (中国农业科学), 2008, 41(10): 3060-3069 (in Chinese with English abstract)
[19]Yang W-X(杨文雄), Yang F-P(杨芳萍), Liang D(梁丹), He Z-H(何中虎), Shang X-W(尚勋武), Xia X-C(夏先春). Molecular characterization of slow-rusting genes Lr34/Yr18 in Chinese wheat cultivars. Acta Agron Sin (作物学报), 2008, 34(7): 1109-1103 (in Chinese with English abstract)
[20]Bai Y-L(白玉路), Sun Q(孙权), Zhang C-Y(张春宇), Cui N(崔娜), Lin F(林凤), Xu S-C(徐世昌), Zhang Z-Y(章振羽), Gao Y(高阳), Xu X-D(徐晓丹). Molecular detection and resistance evaluation of 59 cultivars from the Northwest of the United States to Chinese stripe rust races. Sci Agric Sin(中国农业科学), 2010, 43(6): 1147-1155 (in Chinese with English abstract)
[21]Wu L(伍玲), Tan J(谭君), Zhu H-Z(朱华忠), Wang Z-L(王中烈), Pu X-R(蒲晓蓉). Detection of stripe rust resistant genes of Yr5, Yrl0 and Yr15 in some Sichuan wheat lines by molecular markers. Southwest China J Agric Sci (西南农业学报), 2007, 20(2): 316-320 (in Chinese with English abstract)
[22]Li Z-Q(李振岐), Shang H-S(商鸿生). Wheat Rusts and Disease Control (小麦锈病及其防治). Shanghai: Shanghai Sciences and Technology Publisher, 1989 (in Chinese)
[23]Dubin H J, Johnson R, Stubbs R W. Postulated genes for resistance to stripe rust in selected CIMMYT and related wheat. Plant Dis, 1989, 73: 472-475
[24]Browde L E. Specificity of the Puccinia recondita f. sp. tritici: Triticum aestivum ‘Bulgaria 88’ relationship. Phytopathology, 1973, 63: 524-528
[25]Han B(韩冰), Lin R-M(蔺瑞明), Cao Y-Y(曹远银), Xu S-C(徐世昌). Comparison of the methods of isolating wheat yellow rust genomic DNA. Chin Agric Sci Bull (中国农学通报), 2006, 22(4): 81-83 (in Chinese with English abstract)
[26]Wu L-R(吴立人), Niu Y-C(牛永春). Strategies of sustainable control of wheat stripe rust in China. Sci Agric Sin (中国农业科学), 2000, 33(5): 1-7 (in Chinese with English abstract)
[27]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis, 2004, 88: 896-904
[28]Cao S-Q(曹世勤), Jin S-L(金社林), Jin M-A(金明安), Jia Q-Z(贾秋珍), Li J-P(李继平). Identification of wheat varieties (lines) for resistance to stripe rust in 1994-2002. J Plant Genet Resour(植物遗传资源学报), 2003, 4(2): 119-122 (in Chinese with English abstract)
[29]Li G Q, Li Z F, Yang W Y, Zhang Y, He Z H, Xu S C, Singh R P, Qu Y Y, Xia X C. Molecular tagging of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet, 2006, 112: 1434-1440
[30]Liu T G., Peng Y L, Chen W Q, Zhang Z Y. First detection of virulence in Puccinia striiformis f. sp. tritici in China to resistance genes Yr24 (=Yr26) present in wheat cultivar Chuanmai 42. Plant Dis, 2010, 94: 1163 (Abstract)
[31]Cao S-Q(曹世勤), Luo H-S(骆惠生), Wu C-P(武翠平), Jin S-L(金社林), Wang X-M(王晓鸣), Zhu Z-D(朱振东), Jia Q-Z(贾秋珍), Huang J(黄瑾), Zhang B(张勃), Shang X-W(尚勋武). Postulation of powder mildew resistance genes in 64 wheat cultivars (lines) in Gansu Province. Acta Agron Sin (作物学报), 2010, 36(12): 2107-2115 (in Chinese with English abstract)
[32]Wang K-N(汪可宁), Wu L-R(吴立人), Meng Q-Y(孟庆玉), Xie S-X(谢水仙), Lu D-Y(路端谊), Yuan W-H(袁文焕), Yu X-R(于孝如), Song W-Z(宋位中), Liu S-J(刘守俭), Wang J-Q(王吉庆), Yang J-X(杨家秀), Huang G-M(黄光明), Li Y-F(李艳芳), Yang S-C(杨世诚). On the physiologic specialization of stripe rust of wheat in China during the years 1975-1984. Acta Phytopathol Sin (植物病理学报), 1986, 16(2): 74-85 (in Chinese with English abstract)
[33]Wang J-Q(王吉庆), Lu J-X(陆家兴), Liu S-J(刘守俭), Dai S-K(戴书琨), Liu R-Z(刘汝祯). A preliminary study on the trend of over summer of stripe rust of wheat in Gansu province. Acta Phytopathol Sin (植物病理学报), 1965, 8(1): 1-10 (in Chinese with English abstract)
[34]Li Z-Q(李振岐),Wang M-N(王美楠), Jia M-G(贾明贵), Lu H-P(路和平), Shang H-S(商鸿生), Kang Z-S(康振生). Study on the epidemiology and control strategy of wheat stripe rust in south Gansu. Acta Univ Agric Boreali-Occident (西北农业大学学报), 1997, 25(2): 1-5 (in Chinese with English abstract)
[35]Li Z-Q(李振岐), Shang H-S(商鸿生), Yin S-L(阴省林), Qiang Z-F(强中发), Zhao Y-Q(赵宜谦), Lu H-P(陆和平), Hong X-W(洪锡午), Song W-Z(宋位中), Liu S-J(刘守俭). Studies on the breakdown of resistance of Lovrin cultivars of wheat to stripe rust (Puccinia striiformis West). Sci Agric Sin (中国农业科学), 1984, 8(1): 68-73 (in Chinese with English abstract)
[36]Xie S-X(谢水仙), Wan A-M(万安民), Wu L-R(吴立人), Chen Y-L(陈扬林). Studies of stripe rust spread on autumn wheat in south Gansu Province. Plant Prot(植物保护), 1994, 20(5): 6-8 (in Chinese with English abstract)
[37]Xie S-X(谢水仙), Chen W-Q(陈万权), Chen Y-L(陈扬林), Song W-Z(宋位中), Liu S-J(刘守俭), Yang F-R(杨发荣), Yang S-F(杨世发), Hu J-W(胡箭卫), Dong J-S(董敬生). Occurrence and management of wheat stripe rust (Puccinia striiformis West) in autumn, winter and spring in the south of Gansu Province. Acta Phytophylacica Sin (植物保护学报), 1997, 21(3): 29-34 (in Chinese with English abstract)
[38]Xie S-X(谢水仙), Wan A-M(万安民), Zhang Q-Q(张庆勤), Jin S-L(金社林), Li J-P(李继平), Cao S-Q(曹世勤). Identification and screening of new germplasm resources of wheat resistance to stripe rust and powdery mildew diseases. Plant Prot (植物保护), 1997, 23(6): 3-5 (in Chinese with English abstract)
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