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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1360-1371.doi: 10.3724/SP.J.1006.2011.01360

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Postulation of Stripe Rust Resistance Genes and Analysis of Adult Resistance in 50 Wheat Varieties (Lines) in Gansu Province

CAO Shi-Qin1,2,ZHANG Bo2,LI Ming-Ju3,4,XU Shi-Chang3,LUO Hui-Sheng2,JIN She-Lin2,*,JIA Qiu-Zhen2,HUANG Jin2,SHANG Xun-Wu1   

  1. 1College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 2Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 4 Institute of Agriculture Environment and Resource, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
  • Received:2011-01-19 Revised:2011-04-27 Online:2011-08-12 Published:2011-06-13
  • Contact: 金社林, E-mail: jinshelin@163.com; 尚勋武, E-mail: shangxuwu@163.com

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Abstract: By means of artificial inoculation of 26 Puccinia striiformis isolates with different spectra of pathogenicity at seedling stage, the resistance to strip rust was identified in 50 wheat varieties (lines) from Gansu province, China. In combination with pedigree analysis and resistance reactions of 30 standard varieties with known resistant genes, the resistance genes in the 50 varieties (lines) were postulated. The resistant genes Yr5, Yr10, Yr15, Yr26, and Yr9 (1B/1R) were validated using molecular markers in 43 varieties (lines). The results of resistance gene postulation showed that Zhongliang 25 was carrying Yr3 and unknown genes. The vareity Lantian 20 was postulated to possess Yr3a, Yr4a, Yr16, and unknown genes. The line Y9220-12 was postulated to possess Yr9, YrCle, and unknown genes. The varieties (lines) Lantian 14, Longyuan 932, Longyu 216, and Longyuan 992 were postulated to possess Yr9 and unknown genes. Genes Yr10 and YrMor were postulated in Longjian 9343, 93-Bao 4-4, Tianxuan 43, and Guinong 22. The variety Lantian 19 was postulated to possess Yr12 and unknown resistant genes. Gene Yr26 was postulated in Lantian 17, 95-111-3, 98-178-3-2-4, and 92R137. Fourteen varieties (lines) were detected to be Yr9 (1B/1R) wheat–rye translocation, which accounted for 32.6% of the total varieties. Yr26 was detected in 92R178 and Lantian 17. Other varieties (lines) were controlled by unknown genes. Although most varieties (lines) tested were highly susceptible at seedling stage, some of them showed adult resistance in the observation pool in Longnan, Gansu, China. Ten varieties (lines) including Lantian 10 might possess adult resistance and another 10 varieties (lines) including Lantian 14 might be varieties with slow rusting characters.

Key words: Wheat stripe rust, Resistance gene, Gene postulation, Molecular detection, Gansu Province of China

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