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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1351-1359.doi: 10.3724/SP.J.1006.2011.01351

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Tolerance Analysis of GsNAC20 Gene Linked to Response to Stress in Glycine soja

CAI Hua,ZHU Yan-Ming*,LI Yong,BAI Xi,JI Wei,WANG Dong-Dong,SUN Xiao-Li   

  1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China,
  • Received:2011-01-16 Revised:2011-04-27 Online:2011-08-12 Published:2011-06-13
  • Contact: 朱延明,E-mail:ymzhu2001@yahoo.com.cn

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Abstract: Abiotic stresses, such as salt and drought, affect plant growth, development and reduce crop yield. Isolationof a key regulatory gene linked to response to abiotic-stress and identification of thegenes function areurgently needed. Glycine soja is an excellent material to isolate abiotic stress-related genes because of its high stress tolerance. Plant-specific transcription factor NAC (NAM, ATAF1/2, CUC2) proteins play essential roles in many biological processes such as development, senescence, morphogenesis, and stress signal transduction pathways. It has become a new research focus in the abiotic-stress field. Based on that, we screened a new NAC gene from Glycine soja by yeast one hybrid, which has 99% similarity with NAC20 of Glycine max (EU440353.1), named as GsNAC20. GsNAC20 had typical NAC DNA-binding domain at the N-terminal and transcription activation region at the C-terminal. It can bind to MYB1AT element (the core sequence: AAACCA) in vitro, but no transcriptional activation activity in the yeast assay system, which was consistent with GmNAC20. Localization of GsNAC20 protein was analyzed by transient expression in tobacco epidermis cells and the result showed that GsNAC20 waslocalized in nucleus. Semi-quantitative RT-PCR showed the expression level of GsNAC20 was induced by drought, low temperature and salt stresses, but there existed difference between leaf and root in G. soja. Arabidopsis thaliana plants overexpressing GsNAC20 showed higher sensitivity under salt stress. All results showed that GsNAC20 perhaps is a new member of NAC family in G. soja, and is closely related to salt and drought stresses, so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance in plant.

Key words: Glycine soja, Transcription factor, GsNAC20, Abiotic stress

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