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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (08): 1342-1350.doi: 10.3724/SP.J.1006.2011.01342

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Analysis of 18-C Unsaturated Fatty Acid Contents in Zero-Erucic Rapeseed (Brassica napus L.)

YANG Yan-Yu,YANG Sheng-Qiang,CHEN Zhe-Hong,GUAN Chun-Yun,CHEN She-Yuan,LIU Zhong-Song   

  1. Oilseed Crops Institute, Hunan Agricultural University, Changsha 410128, China
  • Received:2011-01-28 Revised:2011-04-26 Online:2011-08-12 Published:2011-06-13
  • Contact: 刘忠松, E-mail: zsliu48@sohu.com

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Abstract: Rapeseed oil is a major edible oil source for human consumption. Its quality depends on its fatty acid composition. Among fatty acids, 18-C unsaturated fatty acids are most crucial. QTL mapping will pave a way for molecular breeding and cloning genes for fatty acid biosynthesis in rapeseed. In this study, the zero-erucic rapeseed parents HOP (high oleic) and Xiangyou 15 (low oleic) were used to construct a F2 population consisting of 189 individual plants. The construction of a genetic map containing 342 SSR markers and the mapping of QTLs for the 18-C unsaturated fatty acid contents were carried out. Two sampling methods, i.e., half-seed sampling from F2 single seeds and bulk-seed sampling from F2 individual plants (F3 seeds), were used to determine the fatty acid contents by gas chromatography. The correlations of the fatty acid contents between the two sampling methods as well as most of the correlations between these fatty acids were significant. Totally two major QTLs for oleic acid content were detected in the linkage groups (LG) of A5 and C5, which could explain 60-70% of the variance for oleic acid content. The superior one of the two QTLs was mapped in LG A5 and tightly linked to the FAD2 gene. The other one which located in LG C5 was a major QTL newly found for oleic acid content. The marker closest to this QTL was found to be homologous to the marker on the LG A5, implying the QTL on C5 was also associated with the FAD2 gene. Consistent effect of the two QTLs was confirmed using oleic acid contents determined by the two sampling methods. The QTLs for oleic acid content was found to be the ones for the linoleic acid content, which was consistent with the high significant correlation between the two fatty acids. Nevertheless, one more minor QTL for linoleic acid content with a low LOD value was shown on LG A4 using the bulk-seed method. For linolenic acid content, three major QTLs on LG A4, A5, and C4, respectively, were detected consistently using the determinations of the two sampling methods, totally explaining 72-80% of the variance for linolenic acid content. Furthermore, another QTL with a low LOD value, only accounting for 12.42% of variance was detected on LG A4 using the half-seed method.

Key words: Brassica napus, SSR, Fatty acid content, QTL mapping

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