甘蓝型油菜千粒重全基因组关联分析

doi:10.3724/SP.J.1006.2021.04136

Abstract

Abstract:

Thousand-seed weight (TSW) is one of the important component of seed yield. In this study, a collection of 496 representative rapeseed accessions were genotyped by the high-throughput 60K SNP array for TSW in three environments (14NJ, 15TZ, 16TZ). The genome-wide association study (GWAS) of TSW was performed via the MLM (Mixed linear model) and GLM (General linear model). The results showed that the broad sense heritability of TSW was 63.12% in three environments. Six and 61 loci were detected with MLM and GLM, which explained 28.92% and 47.08% of the phenotypic variance, respectively. Combining the common loci between two models, 62 significant loci were obtained and accounted for 47.31% of the phenotypic variance. These loci were distributed on all chromosomes of the genome, with the largest number of 9, 8, and 7 loci detected on Chromosome A07, A03, and C06, respectively. The most significant locus Bn-scaff_17526_1-p1066214 was detected on C09, and accounted for 5.55% and 15.26% of the phenotypic variance in MLM and GLM, respectively. Among them, 21 loci overlapped with previously reported QTLs, of which 8 loci were verified by at least two populations. The remaining 41 loci were newly detected, and many of them had high effects and were detected in multiple environments, such as Bn-A03-p560769, Bn-scaff_15743_1-p599416 and Bn-scaff_15743_1-p590955. Besides, 11 candidates orthologous to documented Arabidopsis seed weight genes, like DGAT, EOD3, AGL61, WRI1, DA2, and RAV1, were found near our GWAS loci. The results are helpful for analyzing the genetic basis of TSW of rapeseed and lay a foundation for studying the regulation mechanism and guiding the genetic improvement of TSW.

Key words: Brassica napus L., 1000-seed weight, yield, GWAS, SNP

ZHANG Chun, ZHAO Xiao-Zhen, PANG Cheng-Ke, PENG Men-Lu, WANG Xiao-Dong, CHEN Feng, ZHANG Wei, CHEN Song, PENG Qi, YI Bin, SUN Cheng-Ming, ZHANG Jie-Fu, FU Ting-Dong. Genome-wide association study of 1000-seed weight in rapeseed (Brassica napus L.)[J].Acta Agronomica Sinica, 2021, 47(4): 650-659.

Figures/Tables 7

Table S1

Information of 496 rapeseed accessions"

材料号 Line No.	材料名称 Name	来源 Origin	材料号Line No.	材料名称 Name	来源 Origin
1	甘油5号 Ganyou 5	中国湖北Hubei, China	2	农林42 Nonglin 42	日本 Japan
3	科里纳 Kelina	国外Unknown	4	淮油6号 Huaiyou 6	中国江苏 Jiangsu, China
5	炎81-2 Yan 81-2	中国重庆Chongqing, China	6	28887	中国重庆Chongqing, China
7	黔油4号 Qianyou 4	中国贵州Guizhou, China	8	黔油331 Qianyou 331	中国贵州 Guizhou, China
9	恩油73 Enyou 73-1-2	中国湖北 Hubei, China	10	牛耳朵 Niuerduo	中国贵州 Guizhou, China
11	880101	中国重庆Chongqing, China	12	SWU40	中国重庆 Chongqing, China
13	SWU42	中国重庆Chongqing, China	14	SWU43	中国重庆 Chongqing, China
15	SWU44	中国重庆Chongqing, China	16	SWU45	中国重庆 Chongqing, China
17	SWU46	中国重庆Chongqing, China	18	SWU47	中国重庆 Chongqing, China
19	SWU48	中国重庆Chongqing, China	20	SWU52	中国重庆 Chongqing, China
21	SWU53	中国重庆Chongqing, China	22	SWU56	中国重庆 Chongqing, China
23	SWU59	中国重庆Chongqing, China	24	SWU65	中国重庆 Chongqing, China
25	SWU82	中国重庆Chongqing, China	26	SWU83	中国重庆 Chongqing, China
27	SWU92	中国重庆Chongqing, China	28	SWU101	中国重庆 Chongqing, China
29	SWU106	中国重庆Chongqing, China	30	SWU108	中国重庆 Chongqing, China
31	川油20 Chuanyou 20	中国四川 Sichuan, China	32	川油18 Chuanyou 18	中国四川Sichuan, China
33	CY12NY-7	中国四川 Sichuan, China	34	CY12Q95406	中国四川Sichuan, China
35	CY12Q8-7	中国四川 Sichuan, China	36	CY12QSZ06	中国四川Sichuan, China
37	CY12QCWH-1	中国四川 Sichuan, China	38	CY12Q95108	中国四川Sichuan, China
39	CY12Q21535-N3	中国四川 Sichuan, China	40	CY12PXW-4	中国四川Sichuan, China
41	CY12PXW-6	中国四川 Sichuan, China	42	CY12PXW-9	中国四川Sichuan, China
43	CY13PXW-17	中国四川 Sichuan, China	44	CY14PXW-18	中国四川Sichuan, China
45	CY15PXW-31	中国四川 Sichuan, China	46	CY16PXW-35	中国四川 Sichuan, China
47	CY17PXW-58	中国四川 Sichuan, China	48	CY18PXW-62	中国四川 Sichuan, China
49	CY19PXW-65	中国四川 Sichuan, China	50	CY20PXW-66	中国四川 Sichuan, China
51	CY21PXW-84	中国四川 Sichuan, China	52	CY12GJ-1	中国四川 Sichuan, China
53	wx1025	中国湖南 Hunan, China	54	wx10213	中国湖南 Hunan, China
55	wx10296	中国湖南 Hunan, China	56	wx10315	中国湖南 Hunan, China
57	10-1043	中国湖南 Hunan, China	58	10-1047	中国湖南 Hunan, China
59	10-1061	中国湖南 Hunan, China	60	10-1070	中国湖南 Hunan, China
61	10-804	中国湖南 Hunan, China	62	10-1358	中国湖南 Hunan, China
63	1472	中国湖南 Hunan, China	64	湘油13号 Xiangyou 13	中国湖南 Hunan, China
65	湘油15号 Xiangyou 15	中国湖南 Hunan, China	66	湘油11号 Xiangyou 11	中国湖南 Hunan, China
67	740	中国湖南 Hunan, China	68	631	中国湖南 Hunan, China
69	613	中国湖南 Hunan, China	70	783	中国湖南 Hunan, China
71	782	中国湖南 Hunan, China	72	YB3	中国湖南 Hunan, China
73	1360	中国湖南 Hunan, China	74	563	中国湖南 Hunan, China
75	WX10329	中国湖南 Hunan, China	76	santana	德国 German
77	1281	中国湖南 Hunan, China	78	509	中国湖南 Hunan, China
79	1368	中国湖南 Hunan, China	80	1322	中国湖南 Hunan, China
81	1252	中国湖南 Hunan, China	82	1321	中国湖南 Hunan, China
83	07022	中国湖北 Hubei, China	84	07094	中国湖北 Hubei, China
85	07016	中国湖北 Hubei, China	86	9F087	中国湖北 Hubei, China
87	97096	中国湖北 Hubei, China	88	97097	中国湖北 Hubei, China
89	07189	中国湖北 Hubei, China	90	07191	中国湖北 Hubei, China
91	07037	中国湖北 Hubei, China	92	RQ011	中国湖北 Hubei, China
93	RR009	中国湖北 Hubei, China	94	RR002	中国湖北 Hubei, China
95	97177	中国湖北 Hubei, China	96	96021	中国湖北 Hubei, China
97	96063	中国湖北 Hubei, China	98	01111	中国湖北 Hubei, China
99	01570	中国湖北 Hubei, China	100	9保22 9Bao 22	中国湖北 Hubei, China
101	01188	中国湖北 Hubei, China	102	02354	中国湖北 Hubei, China
103	02359	中国湖北 Hubei, China	104	02365	中国湖北 Hubei, China
105	93205	中国湖北 Hubei, China	106	93210	中国湖北 Hubei, China
107	Nca	国外 Unknown	108	中双4号 Zhongshuang 4	中国湖北 Hubei, China
109	中双9号 Zhongshuang 9	中国湖北 Hubei, China	110	中双11号 Zhongshuang 11	中国湖北 Hubei, China
111	2011-6200	中国湖北 Hubei, China	112	2011-6308	中国湖北 Hubei, China
113	2011-7103	中国湖北 Hubei, China	114	2012-11526	中国湖北 Hubei, China
115	2012-3448	中国湖北 Hubei, China	116	2012-3546	中国湖北 Hubei, China
117	2012-4531	中国湖北 Hubei, China	118	2012-5086	中国湖北 Hubei, China
119	2012-5113	中国湖北 Hubei, China	120	2012-8327	中国湖北 Hubei, China
121	2012-8355	中国湖北 Hubei, China	122	2012-8380	中国湖北 Hubei, China
123	2012-8998	中国湖北 Hubei, China	124	2012-9323	中国湖北 Hubei, China
125	2012-9354	中国湖北 Hubei, China	126	2012-9380	中国湖北 Hubei, China
127	2012-9478	中国湖北 Hubei, China	128	2012-9542	中国湖北 Hubei, China
129	2012-K8053	中国湖北 Hubei, China	130	R2	中国湖北 Hubei, China
131	希望106 Xiwang 106	中国湖北 Hubei, China	132	阳光198 Yangguang 198	中国湖北 Hubei, China
133	阳光2009 Yangguang 2009	中国湖北 Hubei, China	134	中双10号 Zhongshuang 10	中国湖北 Hubei, China
135	中双12号 Zhongshuang 12	中国湖北 Hubei, China	136	中双6号 Zhongshuang 6	中国湖北 Hubei, China
137	中双7号 Zhongshuang 7	中国湖北 Hubei, China	138	中油589 Zhongyou 589	中国湖北 Hubei, China
139	华油2号 Huayou 2	中国湖北 Hubei, China	140	Major	法国 France
141	华双2号 Huashuang 2	中国湖北 Hubei, China	142	Aurora	德国 German
143	华油13号 Huayou13	中国湖北 Hubei, China	144	Rucabo	德国 German
145	华油3号 Huayou 3	中国湖北 Hubei, China	146	华油14 Huayou 14	中国湖北 Hubei, China
147	宁油1号 Ningyou 1	中国江苏 Jiangsu, China	148	Ceres	德国 German
149	11-9-700	中国湖北 Hubei, China	150	11-9-701	中国湖北 Hubei, China
151	11-9-702	中国湖北 Hubei, China	152	11-9-703	中国湖北 Hubei, China
153	11-9-704	中国湖北 Hubei, China	154	11-9-705	中国湖北 Hubei, China
155	11-9-706	中国湖北 Hubei, China	156	11-9-707	中国湖北 Hubei, China
157	11-P63-5育7 11-P63-5 yu 7	中国湖北 Hubei, China	158	11-P63-8育32 11-P63-8 yu 32	中国湖北 Hubei, China
159	11-P63-3育3 11-P63-3 yu 3	中国湖北 Hubei, China	160	11-P67东 11-P67 Dong	中国湖北 Hubei, China
161	09-P64-1	中国湖北 Hubei, China	162	10-崇23 10-chong23	中国湖北 Hubei, China
163	10-崇24 10-chong 24	中国湖北 Hubei, China	164	10-崇25 10-chong25	中国湖北 Hubei, China
165	10-崇29 10-chong 29	中国湖北 Hubei, China	166	10-崇32 10-chong 32	中国湖北 Hubei, China
167	10-崇33 10-chong 33	中国湖北 Hubei, China	168	10-崇34 10-chong 34	中国湖北 Hubei, China
169	10-江棚2 10-jiangpeng 2	中国湖北 Hubei, China	170	10-江棚3 10-jiangpeng 3	中国湖北 Hubei, China
171	11-育7-103 11-yu 7-103	中国湖北 Hubei, China	172	11-育7-117 11-yu7-117	中国湖北 Hubei, China
173	11-育7-125 11-yu 7-125	中国湖北 Hubei, China	174	11-P74-8	中国湖北 Hubei, China
175	11-P74-13	中国湖北 Hubei, China	176	7-7766-74	中国湖北 Hubei, China
177	P18	中国湖北 Hubei, China	178	64棚-1064 peng-10	中国湖北 Hubei, China
179	圣光77 Shengguang 77	中国湖北 Hubei, China	180	甲预17棚 Jiayu17peng	中国湖北 Hubei, China
181	甲预25棚 Jiayu25peng	中国湖北 Hubei, China	182	甲预16棚 Jiayu16peng	中国湖北 Hubei, China
183	甲预31棚 Jiayu31peng	中国湖北 Hubei, China	184	华双5号 Huashuang5	中国湖北 Hubei, China
185	华双4号 Huashuang 4	中国湖北 Hubei, China	186	甲972Jia972	中国湖北 Hubei, China
187	华双128 Huashuang 128	中国湖北 Hubei, China	188	甲904 Jia904	中国湖北 Hubei, China
189	甲908 Jia 908	中国湖北 Hubei, China	190	甲PF190棚 Jia PF190peng	中国湖北 Hubei, China
191	甲915 Jia 915	中国湖北 Hubei, China	192	甲922 Jia 922	中国湖北 Hubei, China
193	甲951棚 Jia951peng	中国湖北 Hubei, China	194	甲917 Jia 917	中国湖北 Hubei, China
195	甲923 Jia923	中国湖北 Hubei, China	196	甲931 Jia 931	中国湖北 Hubei, China
197	甲预05棚 Jiayu05peng	中国湖北 Hubei, China	198	甲963棚 Jia963peng	中国湖北 Hubei, China
199	沪油12号 Huyou 12	中国上海 Shanghai, China	200	宁油16号 Ningyou 16	中国江苏 Jiangsu, China
201	史力佳 Shilijia	中国江苏 Jiangsu, China	202	杨油6号 Yangyou 6	中国江苏 Jiangsu, China
203	扬油5号 Yangyou 5	中国江苏 Jiangsu, China	204	镇油3号 Zhenyou 3	中国江苏 Jiangsu, China
205	苏油1号 Suyou 1	中国江苏 Jiangsu, China	206	浙油18 Zheyou18	中国浙江 Zhejiang, China
207	浙双72 Zheshuang 72	中国浙江 Zhejiang, China	208	浙双8号 Zheshuang 8	中国浙江 Zhejiang, China
209	浙油758 Zheyou 758	中国浙江 Zhejiang, China	210	沪油14 Huyou 14	中国上海 Shanghai, China
211	沪油18 Huyou 18-2	中国上海 Shanghai, China	212	沪油19 Huyou 19	中国上海 Shanghai, China
213	浙油19 Zheyou 19	中国浙江 Zhejiang, China	214	浙油21 Zheyou 21	中国浙江 Zhejiang, China
215	浙双6号 Zheshuang 6	中国浙江 Zhejiang, China	216	皖油15号 Wanyou 15	中国安徽 Anhui, China
217	皖油16号 Wanyou 16	中国安徽 Anhui, China	218	皖油29 Wanyou 29	中国安徽 Anhui, China
219	AGREV012	德国 German	220	AGREV019	德国 German
221	AGREV021	德国 German	222	Topas	瑞典 Sweden
223	cyclon	丹麦 Denmark	224	伟杰 Weijie	加拿大 Canada
225	四达 Sida	加拿大 Canada	226	至尊 Zhizun	加拿大 Canada
227	海神 Haishen	加拿大 Canada	228	D2	丹麦 Denmark
229	D3	丹麦 Denmark	230	Qu	美国 America
231	11-985	中国青海 Qinghai, China	232	11-504	中国青海 Qinghai, China
233	11-997	中国青海 Qinghai, China	234	11-540	中国青海 Qinghai, China
235	11-1184	中国青海 Qinghai, China	236	10-758	中国青海 Qinghai, China
237	10-1230	中国青海 Qinghai, China	238	10-847	中国青海 Qinghai, China
239	11-1124	中国青海 Qinghai, China	240	垦C1 KenC1	中国陕西 Shaanxi, China
241	P113	中国陕西 Shaanxi, China	242	P158	中国陕西 Shaanxi, China
243	P310	中国陕西 Shaanxi, China	244	P312	中国陕西 Shaanxi, China
245	P668	中国陕西 Shaanxi, China	246	P685	中国陕西 Shaanxi, China
247	A117	中国陕西 Shaanxi, China	248	A172	中国陕西 Shaanxi, China
249	B250	中国陕西 Shaanxi, China	250	B265	中国陕西 Shaanxi, China
251	A109	中国陕西 Shaanxi, China	252	B285	中国陕西 Shaanxi, China
253	C052	中国陕西 Shaanxi, China	254	GY270	中国陕西 Shaanxi, China
255	GY282	中国陕西 Shaanxi, China	256	GY284	中国陕西 Shaanxi, China
257	B262	中国河南 Henan, China	258	A82	中国江西 Jiangxi, China
259	A98	中国陕西 Shaanxi, China	260	B414	中国新疆 Xinjiang, China
261	B308	瑞典 Sweden	262	A97	中国四川 Sichuan, China
263	A148	瑞典 Sweden	264	B431	丹麦 Denmark
265	08-P35	中国湖北 Hubei, China	266	08-P36	中国湖北 Hubei, China
267	09-P32	中国湖北 Hubei, China	268	09-P36	中国湖北 Hubei, China
269	09-P37	中国湖北 Hubei, China	270	10-P10	中国湖北 Hubei, China
271	10-P29	中国湖北 Hubei, China	272	11-P30	中国湖北 Hubei, China
273	12-P24	中国湖北 Hubei, China	274	12-P25	中国湖北 Hubei, China
275	03ⅡB	中国湖北 Hubei, China	276	03Ⅱ3B	中国湖北 Hubei, China
277	03Ⅰ32B	中国湖北 Hubei, China	278	964	中国湖北 Hubei, China
279	DD1	中国湖北 Hubei, China	280	9L302-1	中国湖北 Hubei, China
281	06T9F	中国湖北 Hubei, China	282	03Ⅱ4B	中国湖北 Hubei, China
283	03LF1	中国湖北 Hubei, China	284	9852	中国湖北 Hubei, China
285	9801C	中国湖北 Hubei, China	286	986	中国湖北 Hubei, China
287	876	中国湖北 Hubei, China	288	武164 Wu164	中国湖北 Hubei, China
289	9889	中国湖北 Hubei, China	290	06H7	中国湖北 Hubei, China
291	湖北白花油菜 Hubeibaihuayoucai	中国湖北 Hubei, China	292	南川长角 Nanchuanchangjiao	中国四川 Sichuan, China
293	IMC103	中国重庆 Chongqing, China	294	Oscar	澳大利亚 Australia
295	Sophia	德国 German	296	campina	德国 German
297	Conny	瑞典 Sweden	298	Wesreo	澳大利亚 Australia
299	Wase Chousen	朝鲜 North Korea	300	Gogatsuna	日本 Japan
301	Nakaee Chousen	朝鲜 North Korea	302	Cat.No.117	德国 German
303	90750	中国重庆 Chongqing, China	304	农林43 Nonglin 43	日本
305	西藏油菜 Xizangyoucai	中国西藏 Tibet, China	306	油研2号 Youyan 2	中国贵州
307	矮架早 Aijiazao	中国四川 Sichuan, China	308	SWU41	中国重庆 Chongqing, China
309	SWU49	中国重庆 Chongqing, China	310	SWU54	中国重庆 Chongqing, China
311	SWU57	中国重庆 Chongqing, China	312	SWU60	中国重庆 Chongqing, China
313	SWU61	中国重庆 Chongqing, China	314	SWU62	中国重庆 Chongqing, China
315	SWU63	中国重庆 Chongqing, China	316	SWU64	中国重庆 Chongqing, China
317	SWU66	中国重庆 Chongqing, China	318	SWU67	中国重庆 Chongqing, China
319	SWU68	中国重庆 Chongqing, China	320	SWU69	中国重庆 Chongqing, China
321	SWU70	中国重庆Chongqing, China	322	SWU71	中国重庆 Chongqing, China
323	SWU74	中国重庆 Chongqing, China	324	SWU75	中国重庆 Chongqing, China
325	SWU76	中国重庆 Chongqing, China	326	SWU77	中国重庆 Chongqing, China
327	SWU80	中国重庆 Chongqing, China	328	SWU81	中国重庆 Chongqing, China
329	SWU84	中国重庆 Chongqing, China	330	SWU85	中国重庆 Chongqing, China
331	SWU87	中国重庆 Chongqing, China	332	SWU88	中国重庆 Chongqing, China
333	SWU89	中国重庆 Chongqing, China	334	SWU90	中国重庆 Chongqing, China
335	SWU93	中国重庆 Chongqing, China	336	SWU94	中国重庆 Chongqing, China
337	SWU95	中国重庆 Chongqing, China	338	SWU96	中国重庆 Chongqing, China
339	SWU99	中国重庆 Chongqing, China	340	SWU100	中国重庆 Chongqing, China
341	SWU102	中国重庆 Chongqing, China	342	SWU103	中国重庆 Chongqing, China
343	SWU104	中国重庆 Chongqing, China	344	SWU105	中国重庆 Chongqing, China
345	SWU107	中国重庆 Chongqing, China	346	SWU110	中国重庆 Chongqing, China
347	SWU111	中国重庆 Chongqing, China	348	SWU112	中国重庆 Chongqing, China
349	SWU113	中国重庆 Chongqing, China	350	SWU114	中国重庆 Chongqing, China
351	辐油4号 Fuyou 4	中国重庆 Chongqing, China	352	镇3736 Zhen3736	中国江苏 Jiangsu, China
353	镇2609 Zhen 2609	中国江苏 Jiangsu, China	354	HX0352	中国江苏 Jiangsu, China
355	阳光2009 Yangguang 2009	中国江苏 Jiangsu, China	356	沪油21 Huyou 21	中国上海 Shanghai, China
357	浙双3号 Zheshuang 3	中国浙江 Zhejiang, China	358	浙油21 Zheyou 21	中国浙江 Zhejiang, China
359	皖油20 Wanyou 20	中国安徽 Anhui, China	360	皖油12 Wanyou 12	中国安徽 Anhui, China
361	皖油7号 Wanyou 7	中国安徽 Anhui, China	362	红油3号 Hongyou 3	中国江苏 Jiangsu, China
363	镇油5号 Zhenyou5	中国江苏 Jiangsu, China	364	扬油4号 Yangyou 4	中国上海 Shanghai, China
365	沪油15 Huyou 15	中国上海 Shanghai, China	366	沪油16 Huyou 16	中国上海 Shanghai, China
367	沪油17 Huyou17	中国上海 Shanghai, China	368	浙双72 Zheshuang 72	中国浙江 Zhejiang, China
369	浙双8号 Zheshuang 8	中国浙江 Zhejiang, China	370	浙油50 Zheyou 50	中国浙江 Zhejiang, China
371	中双11 Zhongshuang 11	中国湖北 Hubei, China	372	阳光198 Yangguang198	中国湖北 Hubei, China
373	华航901 Huahang 901	中国湖北 Hubei, China	374	扬J6711 Yang J6711	中国江苏 Jiangsu, China
375	盐6055 Yan 6055	中国江苏 Jiangsu, China	376	杨鉴8号 Yangjian8	中国江苏 Jiangsu, China
377	希望106 Xiwang 106	中国湖北 Hubei, China	378	浙油17号 Zheyou 17	中国浙江 Zhejiang, China
379	中双5号 Zhongshuang 5	中国湖北 Hubei, China	380	中油821 Zhongyou 821	中国湖北 Hubei, China
381	秦油1号 Qinyou 1	中国陕西 Shaanxi, China	382	纬隆88 Weilong 88	中国陕西 Shaanxi, China
383	盐油2号 Yanyou 2	中国江苏 Jiangsu, China	384	秦油5号 Qinyou 5	中国陕西 Shaanxi, China
387	德68-12 De 68-12	中国四川 Sichuan, China	388	Monty	澳大利亚 Australia
389	Oscar	澳大利亚 Australia	390	宁油12 Ningyou 12-1	中国江苏 Jiangsu, China
391	宁油14 Ningyou 14	中国江苏 Jiangsu, China	392	史力丰-1 Shilifeng-1	中国江苏 Jiangsu, China
393	宁油18 Ningyou 18	中国江苏 Jiangsu, China	394	Helios	丹麦 Denmark
395	Hector	加拿大 Canada	396	棉96-203 Mian96-203	中国青海 Qinghai, China
397	青662A Qing662A	中国青海 Qinghai, China	398	699	中国湖北 Hubei, China
399	加拿大2号 Jianada 2	加拿大 Canada	400	中双2号 Zhongshuang 2	中国湖北 Hubei, China
401	WH-12	中国湖北 Hubei, China	402	WH-15	中国湖北 Hubei, China
403	WH-17	中国湖北 Hubei, China	404	WH-19	中国湖北 Hubei, China
405	WH-20	中国湖北 Hubei, China	406	WH-23	中国湖北 Hubei, China
407	WH-24	中国湖北 Hubei, China	408	WH-25	中国湖北 Hubei, China
409	WH-26	中国湖北 Hubei, China	410	WH-27	中国湖北 Hubei, China
411	WH-28	中国湖北 Hubei, China	412	WH-29	中国湖北 Hubei, China
413	WH-30	中国湖北 Hubei, China	414	WH-31	中国湖北 Hubei, China
415	WH-33	中国湖北 Hubei, China	416	WH-37	中国湖北 Hubei, China
417	WH-38	中国湖北 Hubei, China	418	WH-41	中国湖北 Hubei, China
419	WH-42	中国湖北 Hubei, China	420	WH-43	中国湖北 Hubei, China
421	WH-45	中国湖北 Hubei, China	422	WH-49	中国湖北 Hubei, China
423	WH-50	中国湖北 Hubei, China	424	WH-55	中国湖北 Hubei, China
425	WH-56	中国湖北 Hubei, China	426	WH-57	中国湖北 Hubei, China
427	WH-58	中国湖北 Hubei, China	428	WH-59	中国湖北 Hubei, China
429	WH-60	中国湖北 Hubei, China	430	WH-61	中国湖北 Hubei, China
431	WH-62	中国湖北 Hubei, China	432	WH-63	中国湖北 Hubei, China
433	WH-81	中国湖北 Hubei, China	434	WH-83	中国湖北 Hubei, China
435	WH-85	中国湖北 Hubei, China	436	WH-88	中国湖北 Hubei, China
437	WH-93	中国湖北 Hubei, China	438	WH-95	中国湖北 Hubei, China
439	WH-100	中国湖北 Hubei, China	440	WH-127	中国湖北 Hubei, China
441	豫油1号 Yuyou 1	中国河南 Henan, China	442	COBRA	德国 German
443	宁油7号 Ningyou 7	中国江苏 Jiangsu, China	444	Tapidor	法国 France
445	华油6号 Huayou 6	中国湖北 Hubei, China	446	华油12 Huayou 12	中国湖北 Hubei, China
447	Cubs root	朝鲜 North Korea	448	华油10 Huayou 10	中国湖北 Hubei, China
449	Bienvenu	法国 France	450	胜利油菜 Shengliyoucai	中国四川 Sichuan, China
451	ERAKE	波兰 Poland	452	Taisetsu	日本 Japan
453	cresor	加拿大 Canada	454	Daichousen	朝鲜 North Korea
455	comet	瑞典 Sweden	456	Niklas	丹麦 Denmark
457	Askari	德国 German	458	chuosenshu	朝鲜 North Korea
459	WESBROOK	澳大利亚 Australia	460	Suigenshu	朝鲜 North Korea
461	华油4号 Huayou 4	中国湖北 Hubei, China	462	胜利青梗 Shengliqinggeng	中国上海 Shanghai, China
463	密角多头油菜 Mijiaoduotouyoucai	中国上海 Shanghai, China	464	矮箕胜利 Aijishengli	中国上海 Shanghai, China
465	漕泾胜利 Caojingshengli	中国上海 Shanghai, China	466	沪油三号 Huyou 3	中国上海 Shanghai, China
467	勺叶青 Shaoyeqing	中国上海 Shanghai, China	468	非洲油菜乳黄花 Feizhouyoucairuhuanghua	中国上海 Shanghai, China
469	沪激早 Hujizao	中国上海 Shanghai, China	470	漕油2号 Caoyou 2	中国上海 Shanghai, China
471	封顶240 Fengding 240	中国江苏 Jiangsu, China	472	全紫油菜 Quanziyoucai	中国江苏 Jiangsu, China
473	大花球 Dahuaqiu	中国江苏 Jiangsu, China	474	荣选 Rongxuan	中国江苏 Jiangsu, China
475	宁油10号 Ningyou 10	中国江苏 Jiangsu, China	476	宁油8号 Ningyou 8	中国江苏 Jiangsu, China
477	宁油六号 Ningyouliu	中国江苏 Jiangsu, China	478	垛油一号 Duoyou 1	中国江苏 Jiangsu, China
479	淮油12号 Huaiyou 12	中国江苏 Jiangsu, China	480	沛选170 Peixuang 170	中国江苏 Jiangsu, China
481	广德138 Guangde 138	中国安徽 Anhui, China	482	广德8104 Guangde 8104	中国安徽 Anhui, China
483	当油早1号Dangyouzao 1	中国安徽 Anhui, China	484	广德761 Guangde 761	中国安徽 Anhui, China
485	铜陵花叶 Tonglinghuaye	中国安徽 Anhui, China	486	滁610 Chu610	中国安徽 Anhui, China
487	滁107 Chu 107	中国安徽 Anhui, China	488	宿84-6 Su 84-6	中国安徽 Anhui, China
489	皖油早 Wanyouzao	中国安徽 Anhui, China	490	滁油1号 Chuyou 1	中国安徽 Anhui, China
491	滁县白花 Chuxianbaihua	中国安徽 Anhui, China	492	芥65-1 Jie 65-1	中国浙江 Zhejiang, China
493	申黄1号 Shenhuang 1	中国上海 Shanghai, China	494	浙油601 Zheyou 601	中国浙江 Zhejiang, China
495	三高油菜Three high rape	中国浙江 Zhejiang, China	496	早丰一号 Zaofeng 1	中国四川 Sichuan, China

Table S1

Table 1

Fig. 1

Table 2

Fig. 2

Table 3

Significant GWAS loci of 1000-seed weight in GLM (BLUP)"

标记 Marker	染色体 Chr.	位置 Position (bp)	-lg (P)	表型变异 R² (%)	环境 Environment	已报道QTL Reported QTL
Bn-A01-p1156979	A01	760,656	6.37	5.61	15TZ	[35]
Bn-A01-p1440009	A01	1,036,032	5.96	5.21
Bn-A01-p9621623	A01	8,234,343	6.43	5.66		[17,30,36]
Bn-A01-p12222806	A01	10,407,989	7.02	7.03	14NJ
Bn-A01-p15496639	A01	12,941,394	6.64	6.67	16TZ
Bn-A02-p6300284	A02	3,473,319	7.22	7.23	14TZ
Bn-A02-p6564861	A02	3,784,981	8.59	8.53	14NJ/16TZ	[32,35]
Bn-A03-p560769	A03	445,706	9.16	9.08	14NJ/16TZ
Bn-A03-p4352837	A03	3,885,180	6.18	6.22
Bn-A03-p6586436	A03	5,884,508	8.47	8.43	16TZ
Bn-A03-p8851142	A03	8,156,299	7.88	7.86	14NJ/15TZ/16TZ	[32]
Bn-A03-p13758392	A03	12,893,544	6.56	6.60	15TZ
Bn-A03-p14747263	A03	13,916,047	6.57	6.60	14NJ	[35]
Bn-A03-p22125583	A03	20,956,967	5.64	5.60		[32,35]
Bn-A03-p24494224	A03	22,999,825	6.05	6.10		[32]
Bn-A04-p13705636	A04	14,344,669	8.84	8.77	15TZ	[35]
Bn-A05-p2610006	A05	2,720,763	5.69	5.75
Bn-scaff_27198_1-p445589	A06	15,593,147	8.01	7.99	14NJ/15TZ
Bn-A06-p23759352	A06	22,715,814	5.97	6.01
Bn-Scaffold002856-p361	A07	592,982	5.84	5.89	14NJ/16TZ	[14,17,31,36]
Bn-A10-p11601681	A07	2,573,087	6.95	6.15	16TZ	[33]
Bn-A07-p5877587	A07	7,824,442	7.24	6.42	14NJ/15TZ	[13,32]
Bn-A07-p10557557	A07	11,738,166	5.71	5.77	16TZ
Bn-A02-p305007	A07	12,930,679	5.66	4.93	14NJ/15TZ/16TZ
Bn-A07-p11611255	A07	13,756,170	5.95	6.00		[35]
Bn-A07-p16095589	A07	17,996,553	5.96	6.01
Bn-scaff_15743_1-p590955	A07	18,512,786	8.64	8.58	14NJ/15TZ/16TZ
Bn-A07-p17804261	A07	19,646,053	6.20	5.45		[32,38]
Bn-A08-p2675098	A08	2,098,808	6.81	6.83	16TZ
Bn-A08-p10443959	A08	8,372,628	6.30	6.34		[32]
Bn-A08-p13284369	A08	11,051,828	5.89	5.94
Bn-A08-p20343735	A08	17,811,886	8.82	7.90	15TZ
Bn-A09-p7329993	A09	5,542,361	6.20	6.24	14NJ/15TZ	[14,32]
Bn-A10-p15021776	A10	14,967,128	5.78	5.83	15TZ
Bn-A10-p15167470	A10	15,100,392	6.67	6.69	15TZ
Bn-scaff_15803_1-p837307	C01	14,780,320	5.58	5.64	14NJ
Bn-scaff_20942_1-p52095	C02	11,201,048	6.54	6.58	14NJ
Bn-scaff_16002_1-p1803014	C03	12,573,756	6.95	6.97	15TZ
Bn-scaff_26320_1-p269450	C03	30,924,988	5.61	4.89
Bn-scaff_16182_1-p296671	C03	51,992,967	6.41	6.44	14NJ
Bn-scaff_15794_3-p89166	C03	55,716,441	8.28	7.39	14NJ/15TZ
Bn-scaff_17119_1-p148890	C03	56,814,587	9.28	9.19	14NJ/15TZ	[35]
Bn-scaff_16217_1-p597569	C04	21,919,412	5.68	4.96	14NJ
Bn-scaff_18062_1-p229981	C04	31,112,520	6.23	5.47	14NJ/16TZ
标记 Marker	染色体 Chr.	位置 Position (bp)	-lg (P)	表型变异 R² (%)	环境 Environment	已报道QTL Reported QTL
Bn-scaff_15585_1-p1089867	C04	44,500,243	5.88	5.14	16TZ
Bn-scaff_18903_1-p371596	C04	47,531,676	6.87	6.89	16TZ
Bn-scaff_16414_1-p1774629	C05	279,059	7.33	7.33	14NJ
Bn-scaff_20901_1-p2029631	C05	1,973,973	6.47	6.50	14NJ/16TZ
Bn-scaff_20901_1-p1279675	C05	2,707,564	7.36	7.36	14NJ
Bn-scaff_15763_1-p596396	C05	11,699,136	5.71	4.98	16TZ
Bn-scaff_15763_1-p588874	C06	20,160,298	5.85	5.90	16TZ
Bn-scaff_16064_1-p938130	C06	24,703,441	11.57	11.31	14NJ/15TZ/16TZ	[35]
Bn-scaff_15743_1-p599416	C06	27,808,971	9.80	8.80	14NJ/15TZ/16TZ
Bn-scaff_18807_1-p747016	C06	30,110,394	5.69	4.96		[36]
Bn-scaff_16874_1-p411591	C06	31,817,621	6.66	6.69	14NJ/16TZ	[30,36,37]
Bn-scaff_17799_1-p2391172	C06	34,110,582	7.71	6.87	14NJ/16TZ
Bn-scaff_17799_1-p853567	C06	35,737,877	7.70	7.69	14NJ	[35]
Bn-scaff_15705_1-p2279820	C07	35,285,037	6.12	6.16	16TZ
Bn-A08-p10452462	C08	16,678,522	6.88	6.08	14NJ/15TZ/16TZ
Bn-scaff_17526_1-p1066214	C09	1,488,283	15.98	15.26	14NJ/15TZ/16TZ	[32]
Bn-scaff_15576_1-p614226	C09	41,707,720	9.03	8.96	14NJ/16TZ

Table 3

Table 4

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年份/环境 Year/Environment	最小值 Minimum	最大值 Maximum	平均值±标准差 Average ± SD	变异系数 Coefficient of variation
2013/2014 南京Nanjing	2.27	7.63	4.27±0.88	0.21
2014/2015 泰州Taizhou	1.64	5.77	3.65±0.58	0.16
2015/2016 泰州Taizhou	1.50	6.13	3.51±0.64	0.18

标记 Marker	油菜基因 Rapeseed gene	染色体 Chr.	位置 Position	拟南芥同源基因Arabidopsis homolog gene
标记 Marker	油菜基因 Rapeseed gene	染色体 Chr.	位置 Position	基因名称Gene name	基因号Gene ID
Bn-A01-p15496639	BnaA01g20420D	A01	12,306,073	NPC6	AT3G48610
Bn-A03-p22125583	BnaA03g41350D	A03	20,713,211	DGAT	AT3G51520
Bn-A03-p8851142	BnaA03g17130D	A03	8,038,181	TTG2	AT2G37260
Bn-A05-p2610006	BnaA05g36830D	A05	2,713,236	AGL61	AT5G60440
Bn-A07-p11611255	BnaA07g16350D	A07	13,870,810	WRI1	AT3G54320
Bn-A09-p7329993	BnaA09g12210D	A09	6,402,925	GW5	AT1G64500
Bn-scaff_20942_1-p52095	BnaA08g15580D	C02	12,923,783	CLV3	AT2G27250
Bn-scaff_18903_1-p371596	BnaC04g50960D	C04	48,343,005	EOD3	AT2G46660
Bn-scaff_18062_1-p229981	BnaC04g33070D	C04	31,436,211	RAV1	AT1G13260
Bn-scaff_17799_1-p853567	BnaC06g38800D	C06	36,244,755	GRF2	AT1G78300
Bn-scaff_17799_1-p853567	BnaC06g38900D	C06	36,295,477	DA2	AT1G78420
Bn-A08-p10452462	BnaC08g12050D	C08	17,299,420	SHB1	AT4G25350

Genome-wide association study of 1000-seed weight in rapeseed (Brassica napus L.)

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标记 Marker	染色体 Chr.	位置 Position (bp)	-lg (P)	表型变异 R² (%)	环境 Environment	已报道QTL Reported QTL
Bn-A01-p23472380	A01	19,346,196	5.34	6.03	16TZ
Bn-A02-p6300284	A02	3,473,319	4.81	5.41	15TZ
Bn-A02-p6564861	A02	3,784,981	5.38	6.06	14NJ	[32]
Bn-A03-p22125583	A03	20,956,967	4.77	5.36		[32,35]
Bn-scaff_16874_1-p411591	C06	31,817,621	4.33	4.86	16TZ	[30,36,37]
Bn-scaff_17526_1-p1066214	C09	1,488,283	4.93	5.55	16TZ	[32]

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