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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1666-1673.doi: 10.3724/SP.J.1006.2010.01666

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Tolerance Analysis of GsANN Gene Related to Response on Stress in Glycine soja

WANG Xi,LI Yong,ZHU Yan-Ming*,BAI Xi,CAI Hua,JI Wei   

  1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
  • Received:2010-02-10 Revised:2010-05-24 Online:2010-10-12 Published:2010-07-05
  • Contact: ZHU Yan-Ming,E-mail:ymzhu2001@neau.edu.cn E-mail:lagow@163.com

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Abstract: Abiotic stresses, such as salt and drought, affect plant growth, development and reduce crop yield. Glycine soja is an excellent material to clone abiotic stresses related genes because of its high stress tolerance. In previous work, an EST database was constructed using a salt-tolerant variety of G. soja, and a gene expression profile was also obtained by microarray hybridization. Expression of all G. soja ESTs was analyzed, and one 3¢-EST was found to be induced by stress, representing an annexin (ANN) gene, a widely-existing super family which has been found to respond to some kinds of abiotic stresses and plays roles in many important cellular processes including membrane formation, regulation of ion channels, signal transduction and so on. Complete coding sequence of this annexin gene was obtaind with developed 5¢-RACE and named as GsANN. Translation product of GsANN had 57% similarity with ANN4 of Arabidopsis thaliana. GsANN had three ANN domains, with moderate hydrophobicity/hydrophilicity, and no signal peptide or transmembrane segment, which was consistent with other ANNs of other plants. Localization of GsANN protein was analyzed by transient expression in tobacco epidermis cells and the result showed that GsANN localized around plasma membrane unevenly and might form oligomers. Semi-quantitative RT-PCR showed the expression level of GsANN was induced by drought and salt stresses in G. soja leaf. Arabidopsis thaliana plants overexpressing GsANN showed lower survival rate and higher sensitivity under both salt and drought stresses. GsANN is a novel ANN gene and is closely related to salt and drought stresses, so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance of plant.

Key words: Glycine soja, Drought stress, Salt stress, Annexin, GsANN

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