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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1666-1673.doi: 10.3724/SP.J.1006.2010.01666

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Tolerance Analysis of GsANN Gene Related to Response on Stress in Glycine soja

WANG Xi,LI Yong,ZHU Yan-Ming*,BAI Xi,CAI Hua,JI Wei   

  1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
  • Received:2010-02-10 Revised:2010-05-24 Online:2010-10-12 Published:2010-07-05
  • Contact: ZHU Yan-Ming,E-mail:ymzhu2001@neau.edu.cn E-mail:lagow@163.com

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Abstract: Abiotic stresses, such as salt and drought, affect plant growth, development and reduce crop yield. Glycine soja is an excellent material to clone abiotic stresses related genes because of its high stress tolerance. In previous work, an EST database was constructed using a salt-tolerant variety of G. soja, and a gene expression profile was also obtained by microarray hybridization. Expression of all G. soja ESTs was analyzed, and one 3¢-EST was found to be induced by stress, representing an annexin (ANN) gene, a widely-existing super family which has been found to respond to some kinds of abiotic stresses and plays roles in many important cellular processes including membrane formation, regulation of ion channels, signal transduction and so on. Complete coding sequence of this annexin gene was obtaind with developed 5¢-RACE and named as GsANN. Translation product of GsANN had 57% similarity with ANN4 of Arabidopsis thaliana. GsANN had three ANN domains, with moderate hydrophobicity/hydrophilicity, and no signal peptide or transmembrane segment, which was consistent with other ANNs of other plants. Localization of GsANN protein was analyzed by transient expression in tobacco epidermis cells and the result showed that GsANN localized around plasma membrane unevenly and might form oligomers. Semi-quantitative RT-PCR showed the expression level of GsANN was induced by drought and salt stresses in G. soja leaf. Arabidopsis thaliana plants overexpressing GsANN showed lower survival rate and higher sensitivity under both salt and drought stresses. GsANN is a novel ANN gene and is closely related to salt and drought stresses, so it can either be used as a new resource in gene engineering on stress tolerance or be further studied to provide more information for the researches on the mechanism of stress tolerance of plant.

Key words: Glycine soja, Drought stress, Salt stress, Annexin, GsANN

[1] Kreps J A, Wu Y, Chang H S, Zhu T, Wang X, Harper J. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol, 2002, 130: 2129–2141
[2] Seki M, Narusaka M, Ishida J. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J, 2002, 31: 279–292
[3] Yamaguchi-Shinozaki K. Gene networks involved in drought stress response and tolerance. J Exp Bot, 2007, 58: 221–227
[4] Wang Y-F(汪耀富), Yang T-X(杨天旭), Liu G-S(刘国顺), Zhao C-H(赵春华), Wang P(王佩), Chen X-J(陈新建). Differently expressed genes in tobacco leaves under osmotic stress. Acta Agron Sin (作物学报), 2007, 33: 914–920 (in Chinese with English abstract)
[5] Nakashima K, Ito Y, Yamaguchi-Shinozaki K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol, 2009, 149: 88–95
[6] Ma T-C(马廷臣), Chen R-J(陈荣军), Yu R-R(余蓉蓉), Zeng H-L(曾汉来), Zhang D-P(张端品). Global genome expression analysis of transcription factors under PEG osmotic stress in rice root system. Acta Agron Sin (作物学报), 2009, 35: 1030–1037 (in Chinese with English abstract)
[7] Shinozaki K, Yamaguchi-Shinozaki K. Gene expression and signal transduction in water-stress reponse. Plant Physiol, 1997, 115: 327–334
[8] Li J(李杰), Chen L-H(陈丽华), Zhu Y-M(朱延明). Study on transgenic plant of osmotic stress resistance. J Northeast Agric Univ (东北农业大学学报), 2005, 36: 241–248 (in Chinese with English abstract)
[9] Moss S E, Morgan R O. The annexins. Genome Biol, 2004, 5: 219
[10] Na J(那杰), Yang H-Y(杨怀义). Advances in the study of plant annexins. J Liaoning Norm Univ (Nat Sci Edn) (辽宁师范大学学报·自然科学版), 2004, 27: 464–467 (in Chinese with English abstract)
[11] Delmer D P, Potikha T S. Structures and functions of annexins in plants. Cell Mol Life Sci, 1997, 53: 546–553
[12] Clark G B, Sessions A, Eastburn D J, Roux S J. Differential expression of members of the annexin multigene family in Arabidopsis. Plant Physiol, 2001, 126: 1072–1084
[13] Gidrol X, Sabelli P A, Fern Y S, Kush A K. Annexin-like protein from Arabidopsis thaliana rescues delta oxyR mutant of Escherichia coli from H2O2 stress. Proc Natl Acad Sci USA, 1996, 93: 11268–11273
[14] Gorecka K M, Thouverey C, Buchet R, Pikula S. Potential role of annexin AnnAt1 from Arabidopsis thaliana in pH-mediated cellular response to environmental stimuli. Plant Cell Physiol, 2007, 48: 792–803
[15] Boustead C M, Smallwood M, Small H, Bowles D J, Walker J H. Identification of calcium-dependent phospholipid-binding proteins in higher plant cells. FEBS Lett, 1989, 244: 456–460
[16] Hofmann A, Proust J, Dorowski A, Schantz R, Huber R.Annexin 24 from Casicum annuum: X-ray structure and biochemical characterization. J Biol Chem, 2000, 275: 8072–8082
[17] Cantero A, Barthakur S, Bushart T J, Chou S, Morgan R O, Fernandez M P, Clark G B, Roux S J. Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress. Plant Physiol Bioch, 2006, 44: 13–24
[18]Breton G, Vazquez-Tello A, Danyluk J, Sarhan F. Two novel intrinsic annexins accumulate in wheat membranes in response to low temperature. Plant Cell Physiol, 2000, 41: 177–184
[19] Chandran D, Sharopova N, Ivashuta S, Gantt J S, VandenBosch K A, Samac D A. Transcriptome profiling identified novel genes associated with aluminum toxicity, resistance and tolerance in Medicago truncatula. Planta, 2008, 228: 151–166
[20] Ji W, Li Y, Li J, Dai C H, Wang X, Bai X, Cai H, Yang L, Zhu Y M. Generation and analysis of expressed sequence tags from NaCl-treated Glycine soja. BMC Plant Biol, 2006, 6: 4
[21] Sambrook J, Russell D W. Molecular Cloning: A Laboratory Manual, 3rd edn. New York: Cold Spring Harbor Laboratory Press, 2001. pp 1–709
[22] Brigneti G, Voinnet O, Li W X, Ji L H, Ding S W, Baulcombe D C. Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J, 1998, 17: 6739–6746
[23] Yang L(杨靓), Ji W(纪巍), Dai C-H(代翠红), Zhu Y-M(朱延明). Construction of the high throughput technology for screening osmotic stress relevant genes. China Biotechnol (中国生物工程杂志), 2008, 28: 60–64 (in Chinese with English abstract)
[24] Clough S J, Bent A F. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743
[25] Monastyrskaya K, Babiychuk E B, Draeger A. The annexins: spatial and temporal coordination of signaling events during cellular stress. Cell Mol Life Sci, 2009, 66: 2623–2642
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