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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1766-1774.doi: 10.3724/SP.J.1006.2012.01766


Genetic Basis of the High-Tillering Dwarf Trait in the Rice DUS Test Standard Variety Cong’ai 2

WANG Tao1,YUAN Shou-Jiang2,YIN Liang2,ZHAO Jin-Feng1,WAN Jian-Min1,3,LI Xue-Yong1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Shandong Rice Research Institute, Jinan 250100, China; 3 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-03-01 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 李学勇, E-mail: xueyong.li@caas.net.cn; Tel: 010-82109716


Cong’ai 2 (cl2), one of the standard varieties of rice DUS test in China, shows typical high-tillering dwarf phenotype.Genetic analysis showed that this trait is controlled by a single recessive nucleus gene, which was mapped between two InDel markers C4-CL5 and C4-CL4 on the long arm of chromosome 4. Between these two markers, there is a known gene D17/HTD1, mutations in which caused high-tillering dwarf phenotype. Sequencing analysis of the D17/HTD1 allele in cl2 revealed that the 1796th base was substituted from C to T, changing the 599th amino acid from proline to leucine. Another high-tillering dwarf mutant S1-40 was obtained from japonica variety Nipponbare mutagenized by EMS. The 3' splicing site of the 3rd intron of D17/HTD1 was substituted from AG to AA,which caused the formation of two aberrant transcripts in S1-40. D17/HTD1 encodes the carotenoid cleavage dioxygenase 7 (CCD7), which is a key enzyme involved in the biosynthesis of a new plant hormone strigolactones (SLs). Exogenous application of GR24, a synthetic analogue of SLs, inhibited the tillering phenotype of cl2. Phylogenetic analysis revealed that CCD7 homologs existed in almost all plant species. Real-time RT-PCR showed D17/HTD1 was expressed in all rice tissues examined, with the highest expression in stems.

Key words: Cong&rsquo, ai 2, High-tillering dwarf, Strigolactones, Carotenoid-cleavage dioxygenase

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