Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (10): 1766-1774.doi: 10.3724/SP.J.1006.2012.01766

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Basis of the High-Tillering Dwarf Trait in the Rice DUS Test Standard Variety Cong’ai 2

WANG Tao1,YUAN Shou-Jiang2,YIN Liang2,ZHAO Jin-Feng1,WAN Jian-Min1,3,LI Xue-Yong1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Shandong Rice Research Institute, Jinan 250100, China; 3 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-03-01 Revised:2012-06-10 Online:2012-10-12 Published:2012-07-27
  • Contact: 李学勇, E-mail: xueyong.li@caas.net.cn; Tel: 010-82109716

Abstract:

Cong’ai 2 (cl2), one of the standard varieties of rice DUS test in China, shows typical high-tillering dwarf phenotype.Genetic analysis showed that this trait is controlled by a single recessive nucleus gene, which was mapped between two InDel markers C4-CL5 and C4-CL4 on the long arm of chromosome 4. Between these two markers, there is a known gene D17/HTD1, mutations in which caused high-tillering dwarf phenotype. Sequencing analysis of the D17/HTD1 allele in cl2 revealed that the 1796th base was substituted from C to T, changing the 599th amino acid from proline to leucine. Another high-tillering dwarf mutant S1-40 was obtained from japonica variety Nipponbare mutagenized by EMS. The 3' splicing site of the 3rd intron of D17/HTD1 was substituted from AG to AA,which caused the formation of two aberrant transcripts in S1-40. D17/HTD1 encodes the carotenoid cleavage dioxygenase 7 (CCD7), which is a key enzyme involved in the biosynthesis of a new plant hormone strigolactones (SLs). Exogenous application of GR24, a synthetic analogue of SLs, inhibited the tillering phenotype of cl2. Phylogenetic analysis revealed that CCD7 homologs existed in almost all plant species. Real-time RT-PCR showed D17/HTD1 was expressed in all rice tissues examined, with the highest expression in stems.

Key words: Cong&rsquo, ai 2, High-tillering dwarf, Strigolactones, Carotenoid-cleavage dioxygenase

[1]Wang Y, Li J. Branching in rice. Curr Opin Plant Biol, 2011, 14: 94–99



[2]McSteen P, Leyser O. Shoot branching. Annu Rev Plant Biol, 2005, 56: 353–374



[3]Gomez-Roldan V, Fermas S, Brewer P B, Puech-Page`s V, Dun E A, Pillot J P, Letisse F, Matusova R, Danoun S, Portais J C, Bouwmeester H, Be´card G, Beveridge C A, Rameau C, Rochange S F. Strigolactone inhibition of shoot branching. Nature, 2008, 455: 189–194



[4]Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N, Magome H, Kamiya Y, Shirasu K, Yoneyama K, Kyozuka J, Yamaguchi S. Inhibition of shoot branching by new terpenoid plant hormones. Nature, 2008, 455: 195–200



[5]Booker J, Auldridge M, Wills S, McCarty D, Klee H, Leyser O. MAX3/CCD7 is a carotenoid cleavage dioxygenase required for the synthesis of a novel plant signaling molecule. Curr Biol, 2004, 14: 1232–1238



[6]Johnson X, Brcich T, Dun E A, Goussot M, Haurogne K, Beveridge C A, Rameau C. Branching genes are conserved across species. Genes controlling a novel signal in pea are coregulated by other long distance signals. Plant Physiol, 2006, 142: 1014–1026



[7]Simons J L, Napoli C A, Janssen B J, Plummer K M, Snowden K C. Analysis of the DECREASED APICAL DOMINANCE genes of petunia in the control of axillary branching. Plant Physiol, 2007, 143: 697–706



[8]Zou J, Zhang S, Zhang W, Li G, Chen Z, Zhai W, Zhao X, Pan X, Xie Q, Zhu L. The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. Plant J, 2006, 48: 687–698



[9]Bainbridge K, Sorefan K, Ward S, Leyser O. Hormonally controlled expression of the Arabidopsis MAX4 shoot branching regulatory gene. Plant J, 2005, 44: 569–580



[10]Foo E, Bullier E, Goussot M, Foucher F, Rameau C, Beveridge C A. The branching gene RAMOSUS1 mediates interactions among two novel signals and auxin in pea. Plant Cell, 2005, 17: 464–474



[11]Snowden K, Simkin A, Janssen B, Templeton K, Loucas H, Simons J, Karunairetnam S, Gleave A, Clark D, Klee H. The decreased apical dominance1⁄ Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE8 gene affects branch production and plays a role in leaf senescence, root growth, and flower development, Plant Cell, 2005, 17: 746–759



[12]Arite T, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojima M, Sakakibara H, Kyozuka J. DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice. Plant J, 2007, 51: 1019–1029



[13]Booker J, Sieberer T, Wright W, Williamson L, Willett B, Stirnberg P, Turnbull C, Srinivasan M, Goddard P, Leyser O. MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone. Dev Cell, 2005, 8: 443–449



[14]Lin H, Wang R, Qian Q, Yan M, Meng X, Fu Z, Yan C, Jiang B, Su Z, Li J, Wang Y. DWARF27, an iron-containing protein required for the biosynthesis of strigolactones, regulates rice tiller bud outgrowth. Plant Cell, 2009, 21: 1512–1525



[15]Stirnberg P, Furner I J, Leyser O. MAX2 participates in an SCF complex which acts locally at the node to suppress shoot branching. Plant J, 2007, 50: 80–94



[16]Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I, Kyozuka J. Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiol, 2005, 46: 79–86



[17]Arite T, Umehara M, Ishikawa S, Hanada A, Maekawa M, Yamaguchi S, Kyozuka J. d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers. Plant Cell Physiol, 2009, 50: 1416–1424



[18]Liu W, Wu C, Fu Y, Hu G, Si H, Zhu L, Luan W, He Z, Sun Z. Identi?cation and characterization of HTD2: a novel gene negatively regulating tiller bud outgrowth in rice. Planta, 2009, 230: 649–658



[19]Gao Z, Qian Q, Liu X, Yan M, Feng Q, Dong G, Liu J, Han B. Dwarf88, a novel putative esterase gene affecting architecture of rice plant. Plant Mol Biol, 2009, 71: 265–276



[20]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325



[21]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[22]Kamachi K, Yamaya T, Mae T, Ojima K. A role for glutamine synthetase in the recombination of leaf nitrogen during natural senescence in rice leaves. Plant Physiol, 1991, 96: 411–417



[23]Zou J H, Chen Z X, Zhang S Y, Zhang W P, Jiang G H, Zhao X F, Zhai W X, Pan X B, Zhu L H. Characterizations and fine mapping of a mutant gene for high tillering and dwarf in rice (Oryza sativa L.). Planta, 2005, 222: 604–612



[24]Drummond R S, Martínez-Sánchez N M, Janssen B J, Templeton K R, Simons J L, Quinn B D, Karunairetnam S, Snowden K C. Petunia hybrida CAROTENOID CLEAVAGE DIOXYGENASE7 is involved in the production of negative and positive branching signals in petunia. Plant Physiol, 2009, 151: 1867–1877



[25]Vogel J T, Walter M H, Giavalisco P, Lytovchenko A, Kohlen W, Charnikhova T, Simkin A J, Goulet C, Strack D, Bouwmeester H J, Fernie A R, Klee H J. SlCCD7 controls strigolactone biosynthesis, shoot branching and mycorrhiza-induced apocarotenoid formation in tomato. Plant J, 2009, 61: 300–311



[26]Koltai H. Strigolactones are regulators of root development. New Phytol, 2011, 190: 545–549



[27]Cook C E, Whichard L P, Turner B, Wall M E, Egley G H. Germination of witchweed (Striga lutea Lour.): isolation andproperties of a potent stimulant. Science, 1966, 154: 1189–1190



[28]Akiyama K, Matsuzaki K, Hayashi H. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature, 2005, 435: 824–827



[29]Proust H, Hoffmann B, Xie X, Yoneyama K, Schaefe D G, Yoneyama K, Nogué F, Rameau C. Strigolactones regulate protonema branching and act as a quorum sensing-like signal in the moss Physcomitrella patens. Development, 2011, 138: 1531–1539



[30]Schwartz S H, Qin X, Loewen M C. The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching. J Biol Chem, 2004, 279: 46940–46945



[31]Beveridge C A, Kyozuka J. New genes in the strigolactone-related shoot branching pathway. Curr Opin Plant Biol, 2010, 13: 34–39



[32]Zhou H, Liu Q J, Li J, Jiang D G, Zhou L Y, Wu P, Lu S, Li F, Zhu L Y, Liu Z L, Chen L T, Liu Y G, Zhuang C X. Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA. Cell Res, 2012, 22: 649–660



[33]Ding J H, Lu Q, Ouyang Y D, Mao H L, Zhang P B, Yao J L, Xu C G, Li X H, Xiao J H, Zhang Q F. A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice. Proc Natl Acad Sci USA, 2012, 109: 2654–2659

[1] YANG Zheng-Zhao, WANG Zi-Hao, HU Zhao-Rong, XIN Ming-Ming, YAO Ying-Yin, PENG Hui-Ru, YOU Ming-Shan, SU Zhen-Qi, GUO Wei-Long. Comparative analysis of the genomic sequences between commercial wheat varieties Jimai 22 and Liangxing 99 [J]. Acta Agronomica Sinica, 2020, 46(12): 1870-1883.
[2] GUO Tao,HUANG Yong-Xiang**,HUANG Xuan,LIU Yong-Zhu,WANG Hui,ZHANG Jian-Guo,CHEN Zhi-Qiang,WANG Hui. Map-Based Cloning of a Green-Revertible Albino and High-Tillering Dwarf Gene hw-1(t) in Rice [J]. Acta Agron Sin, 2012, 38(08): 1397-1406.
[3] GUO Tao, HUANG Xuan, HUANG Yong-Xiang, LIU Yong-Zhu, ZHANG Jian-Guo, CHEN Zhi-Jiang, WANG Hui. Characterizations of a Mutant Gene hw-1(t) for Green-revertible Albino, High Tillering and Dwarf in Rice (Oryza sativa L.) [J]. Acta Agron Sin, 2012, 38(01): 23-35.
[4] NI Xiao-Wen;YAN Jun;CHEN Xin-Min;XIA Xian-Chun;HE Zhong-Hu;ZHANG Yong;WANG De-Sen;Morten Lillemo. Heritability and Number of Genes Controlling Slow-Mildewing Resis-tance in Wheat Cultivar Lumai 21 [J]. Acta Agron Sin, 2008, 34(08): 1317-1322.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!