Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1397-1406.doi: 10.3724/SP.J.1006.2012.01397

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Map-Based Cloning of a Green-Revertible Albino and High-Tillering Dwarf Gene hw-1(t) in Rice

GUO Tao**,HUANG Yong-Xiang**,HUANG Xuan,LIU Yong-Zhu,WANG Hui,ZHANG Jian-Guo,CHEN Zhi-Qiang*,WANG Hui*   

  1. South China Agricultural University, National Engineering Research Center of Plant Space Breeding, Guangzhou 510642, China
  • Received:2012-01-06 Revised:2012-04-16 Online:2012-08-12 Published:2012-06-04
  • Contact: 王慧, E-mail: wanghui@scau.edu.cn; 陈志强, E-mail: chenlin@scau.edu.cn

PDF

1538

Cited

1

Abstract: In this study, the green-revertible albino and high-tillering dwarf gene hw-1(t) was fine mapped with a 24.9 kb physical distance between two InDel markers, HW36 and HW7 on chromosome 5, where five open reading frames were predicted. Sequencing and restriction enzyme digestion analysis revealed hfa-1 mutant carried a single nucleotide substitution (G→A) in the putative gene LOC_Os4g57320, which resulted in a premature stop codon. Therefore, we deduced that LOC_Os4g57320 is hw-1(t). BLAST analysis indicated that hw-1(t) exits as a single copy in rice genome. Sequence alignment revealed that hw-1(t) was highly homologous to AtIM and SiPTOX, except some variation on transcript and protein structure. Gene expression analysis indicated HW-1(t) was constitutive gene, which did not be affected by light.

Key words: Rice (Oryza sativa L.), Green-revertible albino, High-tillering dwarf, hw-1(t), Cloning

[1]Beale S I. Green genes gleaned. Trends Plant Sci, 2005, 10: 301–312

[2]Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. Defect in non-yellow coloring 3, an alpha/beta hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J, 2009, 59: 940–?952

[3]Terry M J, Kendrick R E. Feedback inhibition of chlorophyll synthesis in the phytochrome chromophore-deficient aurea and yellow-green-2 mutants of tomato. Plant Physiol, 1999, 119: 143?–152

[4]Chen G, Bi Y R, Li N. EGY1 encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development. Plant J, 2005, 41: 364?–375

[5]Kushnir S, Babiychuk E, Storozhenko S, Davey M W, Papenbrock J, Rycke R D, Engler G, Stephan U W, Lange H, Kispal G, Lill R, Van M M. A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starik. Plant Cell, 2001, 13: 89?–100

[6]Wu Z, Zhang X, He B, Sheng S, Wang J, Guo X, Su N, Wang L, Jiang L, Wang C, Zhai H, Wan J. A chlorophyll-deficient rice mutant with impaired chlorophylide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40

[7]Jiang H, Li M, Liang N, Yan H, Wei Y, Xu X, Liu J, Xu Z, Chen F, Wu G. Molecular cloning and analysis of the stay green gene in rice. Plant J, 2007, 52: 197–209

[8]Park S Y, Yu J W, Park J S, Li J, Yoo SC, Lee N Y, Lee S K, Jeong S W, Seo H S, Koh H J, Jeon J S, Park Y I, Paek N C. The senescence-induced stay green protein regulates chlorophyll degradation. Plant Cell, 2007, 19: 1649–1664

[9]Fang J, Chai C, Qian Q, Li C, Tang J, Sun L, Huang Z, Guo X, Sun C, Liu M, Zhang Y, Lu Q, Wang Y, Lu C, Han B, Chen F, Cheng Z, Chu C. Mutations of genes in synthesis of the carotenoid precursors of ABA lead to preharvest sprouting and photo-oxidation in rice. Plant J, 2008, 54: 177–189

[10]Guo T(郭涛), Huang X(黄宣), Huang Y-X(黄永相), Liu Y-Z(刘永柱), Zhang J-G(张建国), Chen Z-Q(陈志强), Wang H(王慧). Characterizations of a mutant gene hw-1(t) for green-revertible albino, high tillering and dwarf in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2012, 38(1): 23–35 (in Chinese with English abstract)

[11]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325

[12]Kumar S, Tamura K, Jakobsen I B, Nei M. MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics, 2001, 17: 1244–1245

[13]Meijer H J G, Divecha N, van den Ende H, Munnik T. Hyperosmotic stress induces rapid synthesis of phosphatidyl-D-inositol 3,5-bisphosphate in plant cells. Planta, 1999, 208: 294–298

[14]Wilson M P, Sun Y, Cao L, Majerus P W. Inositol 1,3,4-trisphosphate 5/6-kinase is a protein kinase that phosphorylates the transcription factors c-Jun and ATF-2. J Biol Chem, 2001, 276: 40998–41004

[15]Ginalski K, Kinch L, Rychlewski L, Grishin N V. DCC proteins: a novel family of thiol-disulfide oxidoreductases. Trends Biochem Sci, 2004, 29: 339–342

[16]Nelson N, Perzov N, Cohen A, Hagai K, Padler V, Nelson H. The cellular biology of proton-motive force generation by V-ATPases. J Exp Biol, 2000, 203: 89–95

[17]Worley C K, Zenser N, Ramos J, Rouse D, Leyser O, Theologis A, Callis1 J. Degradation of Aux/IAA proteins is essential for normal auxin signaling. Plant J, 2000, 21: 553–562

[18]Dieterle M, Zhou Y C, Schafer E, Funk M, Kretsch T. EID1, an F-box protein involved in phytochrome A-specific light signaling. Genes Dev, 2001, 15: 939–944

[19]Carol P, Stevenson D, Bisanz, C, Breitenbach J, Sandmann G, Mache R, Coupland G, Kuntz M. Mutations in the Arabidopsis gene immutans cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. Plant Cell, 1999, 11: 57–68

[20]Wu D, Wright D A, Wetzel C, Voytas D F, Rodermel S. The IMMUTANS variegation locus of Arabidopsis defines a mitochondrial alternative oxidase homolog that functions during early chloroplast biogenesis. Plant Cell, 1999, 11: 43–55

[21]Josse E M, Simkin A J, Gaffé J, Labouré A M, Kuntz M, Carol P. A plastid terminal oxidase associated with carotenoid desaturation during chromoplast differentiation. Plant Physiol, 2000, 123: 1427–1436

[22]Barr J, White W S, Chen L, Bae H, Rodermel S. The GHOST terminal oxidase regulates developmental programming in tomato fruit. Plant Cell Environ, 2004, 27: 840–852

[23]Fu A, Aluru M, Rodermel S R. Conserved active site sequences in Arabidopsis plastid terminal oxidase (PTOX): in vitro and in planta mutagenesis studies. J Biol Chem, 2009, 284: 22625–22632

[24]Fu A, Park S, Rodermel S. Sequences required for the activity of PTOX (IMMUTANS), a plastid terminal oxidase: in vitro and in planta mutagenesis of iron-binding sites and a conserved sequence that corresponds to exon 8. J Biol Chem, 2005, 280: 42489–42496

[25]Wetzel C M, Jiang C Z, Meehan L J, Voytas D F, Rodermel S R. Nuclear-organelle interactions: the immutans variegation mutant of Arabidopsis is plastid autonomous and impaired in carotenoid biosynthesis. Plant J, 1994, 6: 161–175

[26]Aluru M, Bae H, Wu D, Rodermel S R. The Arabidopsis immutans mutation affects plastid differentiation and the morphogenesis of white and green sectors in variegated plants. Plant Physiol, 2001, 127: 67–77

[27]Beveridge C A, Kyozuka J. New genes in the strigolactone-related shoot branching pathway. Curr Opin Plant Biol, 2010, 13: 34–39

[28]Domagalska M A, Leyser O. Signal integration in the control of shoot branching. Nat Rev Mol Cell Biol, 2011, 12: 211–221

[29]Wang Y, Li J. Branching in rice. Curr Opin Plant Biol, 2011, 14: 94–99
[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[3] XIE Qin-Qin, ZUO Tong-Hong, HU Deng-Ke, LIU Qian-Ying, ZHANG Yi-Zhong, ZHANG He-Cui, ZENG Wen-Yi, YUAN Chong-Mo, ZHU Li-Quan. Molecular cloning and expression analysis of BoPUB9 in self-incompatibility Brassica oleracea [J]. Acta Agronomica Sinica, 2022, 48(1): 108-120.
[4] TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing. Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance [J]. Acta Agronomica Sinica, 2021, 47(4): 672-683.
[5] YUE Jie-Ru, BAI Jian-Fang, ZHANG Feng-Ting, GUO Li-Ping, YUAN Shao-Hua, LI Yan-Mei, ZHANG Sheng-Quan, ZHAO Chang-Ping, ZHANG Li-Ping. Cloning and potential function analysis of ascorbic peroxidase gene of hybrid wheat in seed aging [J]. Acta Agronomica Sinica, 2021, 47(3): 405-415.
[6] LI Jing-Lin, LI Jia-Lin, LI Xin-Peng, AN Bao-Guang, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jing, LONG Tuan. Creation and combining ability analysis of recessive genic sterile lines with a new ptc1 locus in rice [J]. Acta Agronomica Sinica, 2021, 47(11): 2173-2183.
[7] YANG Qin-Li, YANG Duo-Feng, DING Lin-Yun, ZHANG Ting, ZHANG Jun, MEI Huan, HUANG Chu-Jun, GAO Yang, YE Li, GAO Meng-Tao, YAN Sun-Yi, ZHANG Tian-Zhen, HU Yan. Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes [J]. Acta Agronomica Sinica, 2021, 47(10): 1854-1862.
[8] HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao. Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate [J]. Acta Agronomica Sinica, 2021, 47(10): 1941-1952.
[9] Tong-Hong ZUO, He-Cui ZHANG, Qian-Ying LIU, Xiao-Ping LIAN, Qin-Qin XIE, Deng-Ke HU, Yi-Zhong ZHANG, Yu-Kui WANG, Xiao-Jing BAI, Li-Quan ZHU. Molecular cloning and expression analysis of BoGSTL21 in self-incompatibility Brasscia oleracea [J]. Acta Agronomica Sinica, 2020, 46(12): 1850-1861.
[10] Li-Na SHANG,Xin-Long CHEN,Sheng-Nan MI,Gang WEI,Ling WANG,Ya-Yi ZHANG,Ting LEI,Yong-Xin LIN,Lan-Jie HUANG,Mei-Dan ZHU,Nan WANG. Phenotypic identification and gene mapping of temperature-sensitive green- revertible albino mutant tsa2 in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2019, 45(5): 662-675.
[11] XUE Xiao-Meng,LI Jian-Guo,BAI Dong-Mei,YAN Li-Ying,WAN Li-Yun,KANG Yan-Ping,HUAI Dong-Xin,LEI Yong,LIAO Bo-Shou. Expression profiles of FAD2 genes and their responses to cold stress in peanut [J]. Acta Agronomica Sinica, 2019, 45(10): 1586-1594.
[12] Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG. Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1021-1031.
[13] Chen-Yu MA,Wei-Min ZHAN,Wen-Liang LI,Meng-Di ZHANG,Zhang-Ying XI. Cloning and Function Analysis of ZmNAOD Gene in Maize [J]. Acta Agronomica Sinica, 2018, 44(10): 1433-1441.
[14] Chao-Xian LIU, Jiu-Guang WANG, Xiu-Peng MEI, Ting-Ting YU, Guo-Qiang WANG, Lian ZHOU, Yi-Lin CAI. Cloning and Imprinting Characterization Analyses of Paternally Expressed Gene ZmVIL1 in Maize Endosperm [J]. Acta Agronomica Sinica, 2018, 44(03): 376-384.
[15] Yun-Fei LIANG, Lin-Cheng ZHANG, Quan-Ming PU, Zhen-Ze LEI, Song-Mei SHI, Yu-Peng JIANG, Xue-Song REN, Qi-Guo GAO. Cloning of BoLH27 Gene from Cabbage and Phenotype Analysis of Transgenic Cabbage [J]. Acta Agronomica Sinica, 2018, 44(03): 397-404.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd