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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2297-2305.doi: 10.3724/SP.J.1006.2012.02297

• RESEARCH NOTES • Previous Articles     Next Articles

Development of Soybean Lines with α'-Subunit or (α'+α)-Subunits Deficiency in 7S Globulin by Backcrossing

SONG Bo1,**,LAN Lan1,**,TIAN Fu-Dong2,TUO Yun1,BAI Yue1,JIANG Zi-Qin1,SHEN Li-Wei1,LI Wen-Bin1,LIU Shan-Shan1,*   

  1. 1 Soybean Research Institute of Northeast Agricultural University, Harbin 150030, China; 2 Agriculture Science Institute of Tongliao City, Tongliao 028015, China
  • Received:2012-03-27 Revised:2012-08-15 Online:2012-12-12 Published:2012-10-08
  • Contact: 刘珊珊, E-mail:ars336699@yahoo.com.cn, Tel: 0451-55190521


Theα'- and α-subunits of 7S globulin, the components of soybean storage protein as well as the main allergens, are important factors affecting the nutritional and processing quality of soybean. It is currently focused to improve the quality of soybean variety by reducing the contents of α' and α-subunits in soybean breeding. In the experiment, we successfully recombined the character of allergenic proteins deficiency into Chinese soybean, using a Japanese variety “Ri B” with (α'+α)-subunits deficiency of 7S globulin (β-conglycinin) as the donor parent, and soybean variety Dongnong 47 with high oil content, wildly planted in Heilongjiang Province of China, as the recurrent parent. The results showed that the plants of α'-subunit-deficient lines (Cc) and (α'+α)-subunit-deficient lines (Cd) could normally grow, set seeds, and stably inherited. The average values of the yield traits in the two lines (Cc, Cd) increased compared with those in the recurrent parent Dongnong47. The average protein contents in the two lines were higher than those in the parents. The protein content reached to 46.7% in the Cd lines. The average oil content in Cc and Cd lines was ranged between that of both parents and a bit more than that of “Ri B”. Fat content in part lines was 20% more than that in Cc and Cd lines, respectively. Total Amino acid contents were increased by introducing a'-subunit and (a'+α)-subunit, amount of arginine (Arg) and asparagine (Asp) in two lines markedly increased. The sulfur-containing amino acid (Met and Cys) contents and the total amino acid content in Cd lines were 0.11% and 0.56% more than those in Dongnong 47, respectively. So the recombined lines have a great potential to enhance food safety and quality, and could be used in soybean breeding for quality.

Key words: Soybeans (Glycine max L. Merr.), Deficiency of allergenic proteins, High sulfur amino acids lines, Backcrossing

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