Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 457-465.doi: 10.3724/SP.J.1006.2014.00457

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping for Plant Height Using Chromosome Segment Substitution Lines in Upland Cotton

HE Rui,SHI Yu-Zhen,ZHANG Jin-Feng,LIANG Yan,ZHANG Bao-Cai,LI Jun-Wen,WANG Tao,GONG Ju-Wu,LIU Ai-Ying,SHANG Hai-Hong,GONG Wan-Kui,BAI Zhi-Chuan1,*,YUAN You-Lu2,*   

  1. 1 Horticulture and Landscape College, Southwest University, Chongqing 400716, China; 2 State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
  • Received:2013-07-25 Revised:2013-11-30 Online:2014-03-12 Published:2014-01-16
  • Contact: 白志川, E-mail: baizhichuan@yahoo.com.cn, Tel: 13983634203; 袁有禄, E-mail: yuanyl@cricaas.com.cn, Tel: 0372-2525371

Abstract:

Chromosome segment substitution lines (BC5F3, BC5F3:4, and BC5F3:5) were developed with G. hirsutum CCRI36 as the recipient parent and G. barbadense Hai1 as the donor parent. In this study, plant height of BC5F3 individuals (2009 in Anyang Henan), and BC5F3:4 lines (2010 in Anyang Henan) and BC5F3:5 lines (2011 in Anyang Henan, Shihezi Xinjiang and Liaoyang Liaoning, respectively) were evaluated. SSR markers were wed to screen 408 BC5F3 individuals. QTLs for plant height were identified by QTL IciMapping V3.2 software. The  results indicated that the transgressive rate of plant height was from 53.43% to 88.97% and totally 16 QTLs for plant height were detected in three generations and five environments, which were mapped on 10 chromosomes, and explained the phenotypic variance from 7.35% to 13.17%. Seven QTLs were detected in at least two environments. qPH-15-19 was also reported in other researches. These stablly expressed QTLs could be applied in the marker assisted selection, fine QTL mapping and gene cloning.

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