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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (02): 259-275.doi: 10.3724/SP.J.1006.2015.00259

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Structural Characterization and Abiotic Stress Response of Soybean TRK-HKT Family Genes

YIN Gui-Xiang,ZHANG Lei*,SHE Mao-Yun   

  1. Crop Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2014-07-02 Revised:2014-09-30 Online:2015-02-12 Published:2014-11-11
  • Contact: 张磊, E-mail: 13955165987@163.com, 佘茂云, E-mail: ahxiaoshe@126.com E-mail:guixiangyin@126.com

Abstract:

Plant TRK-HKT family genes are involved in Na+/K+ transportation and regulation to abiotic stresses. We used six soybean varieties with different potassium use efficiencies (PUE) as materials, cloned four soybean TRK-HKT family genes (GmHKT1;1, GmHKT1;2, GmHKT1;3, and GmHKT1;4) via in silico, and explored the genes structure and expression under low potassium treatment and abiotic stresses with qRT-PCR technique. The results showed that the expression level of GmHKT1;2 was higher than those of the other three members in the roots of soybean seedlings under low potassium stress, which was more obvious in the roots of the soybean varieties with high PUE. Meanwhile, GmHKT1;2 showed high response to various abiotic stresses (chilling, drought, high salinity, and ABA). Protein structure prediction showed that only GmHKT1;2 contains four MPM domains and a "funnel-like" structure of four conserved amino acid residues spatially, which acted as K+/Na+ transport channel and provided energy for transportation, together with the adjacent ATP binding domain. Analysis on gene structure indicated that there are three exons and two introns in all four members with a significant difference in the size of exon I and intron I, resulting in the genomic DNA (gDNA) difference in lenghth of the different GmHKT genes. Promoter analysis revealed that upstream promoter elements of soybean TRK-HKT family genes contained important cis-acting regulatory elements involved in the functional target to seed-specific expression, and response to hormone and diverse abiotic stresses. In evolution, soybean TRK-HKT family genes belonged to clade I with conserved Ser–Gly–Gly–Gly motif.

Key words: Soybean, TRK-HKT family, Molecular characterization, Abiotic stress

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