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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (09): 1372-1383.doi: 10.3724/SP.J.1006.2015.01372


Genetic Analysis and QTL Mapping of Isoflavone Contents and Its Components in Soybean

LIANG Hui-Zhen1, YU Yong-Liang1, YANG Hong-Qi1, XU Lan-Jie1, DONG Wei1, NIU Yong-Guang1, ZHANG Hai-Yang1, LIU Xue-Yi2, FANG Xuan-Jun3   

  1. 1 Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 Industrial Crop Institute, Shanxi Academy of Agricultural Sciences, Fenyang 032200, China; 3 Hainan Provincial Institute of Tropical Agriculture Resources, Sanya 572025, China
  • Received:2015-03-03 Online:2015-09-12 Published:2015-09-12

Abstract: A set of 447 recombinant inbred lines (RILs) derived from the cross between cultivars Jingdou 23 (female parent) and Huibuzhi (semi-wild, male parent) was used to construct a new map. Isoflavone content and its components were quantitatively and qualitatively evaluated by using high performance liquid chromatography (HPLC). We analyzed inheritance and detected QTLs for isoflavone content and its components in soybean seeds using major gene plus polygene mixed inheritance analysis and WinQTLCart 2.5 composite interval mapping. The results showed that daidzin, daidzein, genistein, genistin, glycitin, and total isoflavone contents were controlled by four, four, two, three, two and two main-genes, respectively. However, polygene effects were not detected in the study. Forty-four quantitative trait loci (QTLs) for isoflavone contents and its components were mapped, including ten for daidzin, nine for genistein, four for daidzein, seven for glycitin, eight for genistin, and six for total isoflavone content. The stable QTLs related to daidzin, genistin, glycitin, isoflavone content were respectively detected to be located in the intervals of satt430-satt359, satt038-satt570, satt197-sat_128, and satt249-satt285 during two years, which could be used in marker-assisted selection (MAS) for soybean breeding.

Key words: Soybean, Isoflavone content, Genetic analysis, Quantitative trait loci

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