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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 641-647.doi: 10.3724/SP.J.1006.2016.00641


Analysis of Interspecific SNPs in Sweetpotato Using a Reduced-Representation Genotyping Technology

SHI Xuan1,WANG Ru-Yuan1,TANG Jun2,LI Zong-Yun1,*,LUO Yong-Hai1,*   

  1. 1 School of Life Science, Jiangsu Normal University, Xuzhou 221116, China; 2 Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences / National Sweetpotato Improvement Centre, Xuzhou 221121, China
  • Received:2015-10-19 Revised:2016-01-11 Online:2016-05-12 Published:2016-02-18
  • Contact: Luo Yonghai, E-mail: yhluo@jsnu.edu.cn; 李宗芸, E-mail: zongyunli@jsnu.edu.cn E-mail:shixuanluck@163.com
  • Supported by:

    This study was supported by the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Project on Crop Conservation Funded by the Ministry of Agriculture of China (2015NWB006), the General Projects Funded by Natural Science Foundation of Jiangsu Province (BK2012579, BK20141146), and the Major Project Funded by Natural Science Foundation of Jiangsu Higher Education Institutions (12KJA180001).


Xushu18 (2x), Nancy Hall (6x), I. trifida (2x) 4597-10, I. trifida (2x) 4597-21, I. trifida (4x), I. trifida (6x), I. temussima (2x), and I. littorallis (2x) were used as experimental materials for sequencing by specific-locus amplified fragment sequencing (SLAF-seq), a high-throughput reduced-representation genotyping technology. In total, 724 589 SLAF tags were obtained and 40 765 SNPs were identified out of 35 310 polymorphic SLAF tags. A total of 40 765 single nucleotide polymorphisms (SNPs) were obtained by sequence analysis. Population structure and phylogenetic relationship of eight germplasm were analyzed using the SNP dataset, which suggests that SLAF-seq can be used to develop large-scale SNPs for population genetic analysis, effectively and economically. Our analysis revealed that the relationship between sweet potato cultivars and the wild species I. trifida is closer. These results provide empirical data for further study of the origin of sweet potato.

Key words: Sweetpotato, SLAF-seq, Molecular marker, SNP, Phylogenetic analysis

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