Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 1021-1029.doi: 10.3724/SP.J.1006.2017.01021

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning, Characteristics and Regulating Role in Thermotolerance of Heat Shock Transcription Factor (ZmHsf25) in Zea mays L.

ZHAO Li-Na1,2,**,DUAN Shuo-Nan1,**,ZHANG Hua-Ning1,GUO Xiu-Lin1,*,LI Guo-Liang1,*   

  1. 1 Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China; 2 College of Life Science, Hebei Normal University, Shijiazhuang 050024, China?
  • Received:2016-11-08 Revised:2017-03-02 Online:2017-07-12 Published:2017-03-17
  • Contact: Li Guoliang,E-mail: guolianglili@163.com, Tel: 0311-87652117;LI Guoliang,E-mail: myhf2002@163.com, Tel: 0311-87269032
  • Supported by:

    This study was financially supported by the Natural Science Foundation of Hebei Province (C2017301065), the Doctoral Foundation of Hebei Province (2017039349), the Bohai Barn Science and Technology Project (F16C14001) and the High-level Talent Project of Hebei Province (A201500130).

Abstract:

Heat shock transcription factors (Hsfs) are key components of signal transduction pathways involved in the activation of genes in response to heat shock stress in plants. There are at least 30 Hsf members in maize and seven of which belong to class B. In our previous work, we obtained ZmHsf06, which belongs to subclass A1, and investigated the characteristics of expression, subcellular localization, and regulating roles in thermotolerance and drought-stress tolerance of ZmHsf06. In the present study, ZmHsf25 was isolated from maize (Zea mays L.) young leaves treated by heat shock at 42°C for 1 h using homologous cloning methods. The sequencing analysis showed that the coding sequence (CDS) of ZmHsf25 was 957 bp and encoded a protein of 318 amino acids. The amino acid sequence analysis demonstrated that ZmHsf25 contained a DNA-binding domain (DBD), a nuclear localization signal (NLS) of KRLR peptide and a nuclear export signal (NES) of VLTLSV peptide. The identity of amino acid between ZmHsf25 and Sb060g025710 of sorghum was the highest, which was 92%. ZmHsf25 was expressed in multiple tissues and organs of maize, and transcription expression level of ZmHsf25 was the highest in pollens compared with root, stem, functional leaf, immature embryo and ear. qRT-PCR results showed that ZmHsf25 was up-regulated by 42°C heat shock in both leaves and roots. Under normal conditions, ZmHsf25 was down-regulated by both SA and H2O2, but significantly up-regulated by heat stress at 42°C. Through transient reporter assay with onion (Allium cepa L.) epidermal cells, we found that ZmHsf25 was localized in nuclei. ZmHsf25 overexpressed yeast showed stronger thermotolerance than the controls after heat shock (HS), though yeast thermotolerance was both decreased by HS. The results revealed that ZmHsf25 perhaps is one of downstream elements of SA signal pathway to play a key role in regulating the response to heat stress and pollen development. These results will provide a theoretical basis for analyzing biological characteristics and functions of maize Hsf members further.

Key words: Maize, ZmHsf25, Expression, Subcelullar-localization, Thermotolerance

[1] Nover L, Scharf K D, Gagliardi D, Vergne P, Czarnecka-Verner E, Gurley W B. The HSF world: classification and properties of plant heat stress transcription factors. Cell Stress Chaperones, 1996, 1: 215–223 [2] Nover L, Bharti K, D?ring P, Mishra S K, Ganguli A, Scharf K D. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need. Cell Stress Chaperones, 2001, 6: 177–189 [3] Guo M, Liu H J. Ma X, Luo D X, Gong Z H, Lu M H. The plant heat stress transcription factors (HSFs): structure, regulation and function in response to aboitic stresses. Front Plant Sci, 2016, 7: 114 [4] Scharf K D, Rose S, Zott W. Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF. EMBO J, 1990, 9: 4495–4501 [5] Xue G P, Sadat S, Drenth J, Mclntyre C L. The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes. J Exp Bot, 2014, 65: 539–557 [6] Liu H C, Charng Y Y. Common and distinct functions of Arabidopsis class A1 and A2 heat shock factors in diverse abiotic stress responses and development. Plant Physiol, 2013, 163: 276–290 [7] Mishra S K, Tripp J, Winkelhaus S, Tschiersch B, Theres K, Nover L, Scharf K D. In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes and Development, 2002, 16: 1555–1567 [8] Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doüring P. Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization. Plant J, 2004, 39: 98–112 [9] Wunderlich M, Gro?-Hardt R, Sch?ff F. Heat shock factor HSFB2a involved in gametophyte development of Arabidopsis thaliana and its expression is controlled by a heat-inducible long non-coding antisense RNA. Plant Mol Biol, 85: 541–550 [10] Ikeda M, Mitsuda N, Ohme-Takagi M. Arabidopsis HsfB1 and HsfB2b act as repressors for the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance. Plant Physiol, 2011, 157: 1243–1254 [11] Ma H, Wang C T, Yang B, Cheng H Y , Wang Z, Mijiti A, Ren C, Qu G H, Zhang H, Ma L. CarHSFB2, a Class B heat shock transcription factor, is involved in different developmental processes and various stress responses in chickpea (Cicer Arietinum L.). Plant Mol Biol Rep, 2016, 34: 1–14 [12] Kumar M, Busch W, Birke H, Kemmerling B, Nürnberger T, Sch?ffl F. Heat shock factors HsfB1 and HsfB2b are involved in the regulation of Pdf1.2 expression and pathogen resistance in Arabidopsis. Mol Plant, 2009, 2: 152–165 [13] Zhu X, Thalor S K, Takahashi Y, Berberich T, Kusano T. An inhibitory effect of the sequence-conserved upstream open-reading frame on the translation of the main open-reading frame of HsfB1 transcripts in Arabidopsis. Plant Cell Environ, 2012, 35: 2014–2030 [14] Kolmos E, Chowa B Y, Pruneda-Pazb J L, Kay S A. Kolmos HsfB2b-mediated repression of PRR7 directs abiotic stress responses of the circadian clock. Proc Natl Acad Sci USA, 2014, 111: 16173–16177 [15] Bharti K, Von KoskullD?ring P, Bharti S, Kumar P, Tintschlk?rbitzer A, Treuter E, Nover L. Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with HAC1/CBP. Plant Cell, 2004, 16: 1521–1535 [16] Hahn A, Bublak D, Schleiff E, Scharf K D. Crosstalk between Hsp90 and Hsp70 chaperones and heat stress transcription factors in tomato. Plant Cell, 2011, 23: 741–755 [17] Begum T, Reuter R, Sch?ff F. Overexpression of AtHsfB4 induces specific effects on root development of Arabidopsis. Mech Dev, 2012, 130: 54–60 [18] Mittal D, Chakrabarti S, Sarkar A, Singh A, Grover A. Heat shock factor gene family in rice: genomic organization and transcript expression profiing in response to high temperature, low temperature and oxidative stresses. Plant Physiol Biochem, 2009, 47: 785–795 [19] 李慧聪, 李国良, 郭秀林. 玉米热激转录因子基因ZmHsf-Like对逆境胁迫响应的信号途径. 作物学报, 2014, 40: 622–628 Li H C, Li G L, Guo X L. Signal transduction pathway of ZmHsf-Like gene responding to different abiotic stresses. Acta Agron Sin, 2014, 40: 622–628 (in Chinese with English abstract). [20] Lin Y X, Jiang H Y, Chu Z X, Tang X L, Zhu S W, Cheng B J. Genome-wide identifiation, classifiation and analysis of heat shock transcription factor family in maize. BMC Genomics, 2011, 12: 76–89 [21] Li H X, Fan R C, Li L B, Wei B, Li G L, Gu L Q, Wang X P, Zhang X Q. Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat. Plant Cell Environ, 2014, 37: 1561–1573 [22] 李慧聪, 李国良, 郭秀林. 玉米热激转录因子基因(ZmHsf06)的克隆、表达和定位分析. 农业生物技术学报, 2015, 23(1): 41–51 Li H C, Li G L, Guo X L. Cloning, expression characteristics and subcellular-location of heat shock transcription factor ZmHsf06 in Zea mays. J Agric Biotechnol, 2015, 23: 41–51 (in Chinese with English abstract). [23] Gietz D, Jean A S, Woods R A, Schiestl R H. Improved method for high transformation of intact yeast cells. Nucleic Acids Res, 1992, 20: 1425 [24] Li H C, Zhang H N, Li G L, Liu Z H, Zhang Y M, Zhang H M. Expression of maize heat shock transcription factor gene ZmHsf06 enhances the thermotolerance and drought-stress tolerance of transgenic Arabidopsis. Funct Plant Biol, 2015, 42: 1080–1090 [25] Czarnecka-Verner E, Pan S, Salem T, Gurley W B. Plant class B HSFs inhibit transcription and exhibit affinity for TFIIB and TBP. Plant Mol Biol, 2004, 56: 57–75 [26] Ikeda M, Ohme-Takagi M. A novel group of transcriptional repressors in Arabidopsis. Plant Cell Physiol, 2009, 50: 970–975 [27] Bharti K, von Koskull-D?ring P, Bharti S, Kumar P, Tintschl-Korbitzer A, Treuter E, Nover L. Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1. Plant Cell, 2004, 16: 1521–1535 [28] Xiang J, Ran J, Zou J, Zhou X, Liu A. Heat shock factor OsHsfB2b negatively regulates drought and salt tolerance in rice. Plant Cell Rep, 2013, 32: 1795–1806 [29] Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S, Ward E, Ryals J. Requirement of salicylic acid for the induction of systemic acquired resistance. Science, 1993, 261: 6 [30] Larkindale J, Hall J D, Knight M R, Vierling E. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol, 2005, 138: 882–886 [31] Snyman M, Cronjé M J. Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings. J Exp Bot, 2008, 59: 2125–2132 [32] Ayarpadikannan S, Chung E, Cho C W, So H A, Kim S O, Jeon J M, Kwak M H, Lee S W, Lee J H. Exploration for the salt stress tolerance genes from a salt-treated halophyte, Suaeda asparagoides. Plant Cell Rep, 2012, 31: 35–48

[1] CHEN Song-Yu, DING Yi-Juan, SUN Jun-Ming, HUANG Deng-Wen, YANG Nan, DAI Yu-Han, WAN Hua-Fang, QIAN Wei. Genome-wide identification of BnCNGC and the gene expression analysis in Brassica napus challenged with Sclerotinia sclerotiorum and PEG-simulated drought [J]. Acta Agronomica Sinica, 2022, 48(6): 1357-1371.
[2] WANG Dan, ZHOU Bao-Yuan, MA Wei, GE Jun-Zhu, DING Zai-Song, LI Cong-Feng, ZHAO Ming. Characteristics of the annual distribution and utilization of climate resource for double maize cropping system in the middle reaches of Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(6): 1437-1450.
[3] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[4] CHEN Jing, REN Bai-Zhao, ZHAO Bin, LIU Peng, ZHANG Ji-Wang. Regulation of leaf-spraying glycine betaine on yield formation and antioxidation of summer maize sowed in different dates [J]. Acta Agronomica Sinica, 2022, 48(6): 1502-1515.
[5] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[6] SHAN Lu-Ying, LI Jun, LI Liang, ZHANG Li, WANG Hao-Qian, GAO Jia-Qi, WU Gang, WU Yu-Hua, ZHANG Xiu-Jie. Development of genetically modified maize (Zea mays L.) NK603 matrix reference materials [J]. Acta Agronomica Sinica, 2022, 48(5): 1059-1070.
[7] YAO Xiao-Hua, WANG Yue, YAO You-Hua, AN Li-Kun, WANG Yan, WU Kun-Lun. Isolation and expression of a new gene HvMEL1 AGO in Tibetan hulless barley under leaf stripe stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1181-1190.
[8] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[9] JIN Min-Shan, QU Rui-Fang, LI Hong-Ying, HAN Yan-Qing, MA Fang-Fang, HAN Yuan-Huai, XING Guo-Fang. Identification of sugar transporter gene family SiSTPs in foxtail millet and its participation in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 825-839.
[10] YUAN Da-Shuang, DENG Wan-Yu, WANG Zhen, PENG Qian, ZHANG Xiao-Li, YAO Meng-Nan, MIAO Wen-Jie, ZHU Dong-Ming, LI Jia-Na, LIANG Ying. Cloning and functional analysis of BnMAPK2 gene in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(4): 840-850.
[11] XU Jing, GAO Jing-Yang, LI Cheng-Cheng, SONG Yun-Xia, DONG Chao-Pei, WANG Zhao, LI Yun-Meng, LUAN Yi-Fan, CHEN Jia-Fa, ZHOU Zi-Jian, WU Jian-Yu. Overexpression of ZmCIPKHT enhances heat tolerance in plant [J]. Acta Agronomica Sinica, 2022, 48(4): 851-859.
[12] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[13] YAN Yu-Ting, SONG Qiu-Lai, YAN Chao, LIU Shuang, ZHANG Yu-Hui, TIAN Jing-Fen, DENG Yu-Xuan, MA Chun-Mei. Nitrogen accumulation and nitrogen substitution effect of maize under straw returning with continuous cropping [J]. Acta Agronomica Sinica, 2022, 48(4): 962-974.
[14] XU Ning-Kun, LI Bing, CHEN Xiao-Yan, WEI Ya-Kang, LIU Zi-Long, XUE Yong-Kang, CHEN Hong-Yu, WANG Gui-Feng. Genetic analysis and molecular characterization of a novel maize Bt2 gene mutant [J]. Acta Agronomica Sinica, 2022, 48(3): 572-579.
[15] ZHOU Yue, ZHAO Zhi-Hua, ZHANG Hong-Ning, KONG You-Bin. Cloning and functional analysis of the promoter of purple acid phosphatase gene GmPAP14 in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 590-596.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!