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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (05): 783-789.doi: 10.3724/SP.J.1006.2018.00783

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles    

Characteristics of Ultra-weak Photon Emission from Sorghum Seeds during Germination

Yan-Yan ZHAO1,*(), Gang XI2   

  1. 1 Zhengzhou University of Industrial Technology, Zhengzhou 451100, Henan, China
    2 Department of Applied Physics, Faculty of Sciences, Xi’an University of Technology, Xi’an 710048, Shaanxi, China;
  • Received:2017-09-08 Accepted:2018-01-08 Online:2018-05-20 Published:2018-01-29
  • Contact: Yan-Yan ZHAO E-mail:zhaoyan5416@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31471412, 51277151).

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Abstract:

Ultra-weak photon radiation from germinated seed includes spontaneous photon emission of germinated seeds in the dark and delayed photon radiation induced by external light. In order to study dynamical feature and biological significance of ultra-weak photon radiation from sorghum during germination, 50 μg mL-1 protein synthesis transcription inhibitor actinomycin D (AMD), 100 μg mL-1 protein synthesis translation inhibitor cycloheximide (CHM) and 650 μg mL-1 respiration inhibitor were adopted to treat germinated sorghum seeds separately. There was a positive correlation (correlation coefficient was 0.93121) between the spontaneous photon emission intensity of germinated sorghum and seed fresh mass. AMD and CHM partially inhibited while NaN3 almost completely inhibited the increase of fresh mass of sorghum seeds in germination process. It indicated that the spontaneous photon emission intensity from germinated sorghum seed can be taken as a signal of germination status from which RNA/DNA synthesis reaction is one of the sources and respiratory metabolism is the main source. The characteristic of delayed photon emission from germinated sorghum seeds can be expressed quantitatively by dynamical parameters such as initial photon number, coherence time and the integrated intensity of delayed photon emission. The cell metabolism and its changes in germination process of sorghum seeds can be understand and evaluated through a real-time, sensitive and non-destructive analysis of the integrated intensity of delayed photon emission from germinated sorghum seeds.

Key words: sorghum, germination, ultra-weak photon radiation, protein synthesis inhibitor, respiratory metabolic inhibitor, non-destructive test

Fig. 1

Changes of fresh mass of sorghum seeds during germination"

Fig. 2

Changes of spontaneous photon emission of sorghum seeds during germination"

Table 1

Dynamical parameters for delayed photon emission of sorghum seeds during germination in control group"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 237.28 0.26 0.84 0.993
1 8.57 256.05 0.27 0.87 0.992
2 9.51 298.72 0.30 0.95 0.996
3 10.38 333.00 0.32 0.96 0.992
4 10.39 337.77 0.35 0.97 0.995
5 10.17 323.95 0.32 0.96 0.991
6 10.41 344.55 0.34 0.97 0.995
7 10.27 331.29 0.33 0.96 0.996
8 10.42 353.76 0.38 0.97 0.996

Table 2

Dynamical parameters for delayed photon emission of sorghum seeds during germination in NaN3 treatment group"

萌发时间
Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon
number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量
Attenuation
coefficient,
β		 拟合优度
Determination
coefficient,
R2
0 7.64 234.28 0.25 0.83 0.992
1 7.78 245.65 0.26 0.84 0.996
2 7.96 269.56 0.27 0.86 0.993
3 8.02 278.51 0.28 0.87 0.991
4 7.56 233.87 0.23 0.81 0.991
5 7.16 228.73 0.22 0.78 0.991
6 7.27 229.76 0.23 0.80 0.994
7 7.16 223.19 0.21 0.80 0.993
8 7.18 226.09 0.22 0.78 0.996

Table 3

Dynamical parameters for delayed photon emission of sorghum seeds during germination in AMD treatment group"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间
Coherence
time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.44 0.26 0.84 0.994
1 8.17 252.95 0.26 0.86 0.994
2 8.67 290.64 0.29 0.93 0.996
3 9.13 318.03 0.32 0.95 0.992
4 9.15 322.93 0.32 0.95 0.994
5 8.98 305.41 0.32 0.95 0.995
6 9.11 317.80 0.33 0.95 0.994
7 9.06 309.35 0.32 0.94 0.992
8 9.19 324.50 0.34 0.94 0.992

Table 4

Dynamical parameters for delayed photon emission of sorghum seeds during germination in CHM treatment group"

萌发时间Germination time
(d)		 自发光子辐射Spontaneous photon emission, ISL
(counts s-1)		 初始光子数
Initial photon number, I0
(counts s-1)		 相干时间Coherence time, τ
(s)		 衰减常量Attenuation
coefficient,
β		 拟合优度Determination
coefficient,
R2
0 7.64 235.07 0.26 0.83 0.993
1 7.93 249.44 0.26 0.85 0.990
2 8.31 281.77 0.29 0.92 0.994
3 8.64 311.75 0.30 0.92 0.993
4 8.73 314.14 0.31 0.93 0.991
5 8.56 307.29 0.31 0.93 0.996
6 8.70 312.99 0.31 0.94 0.992
7 8.61 299.47 0.31 0.93 0.993
8 8.67 318.98 0.32 0.93 0.995

Fig. 3

Changes of the integrated intensity of delayed photon emission from sorghum seeds during germination"

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