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Preliminary study on the regulation of cassava plant type by MeLAZY1c gene

WANG Jia-Xiang1, YU Xue-Ting1, LI Meng-Tao1, MAI Wei-Tao1, CHEN Xin2,3,*,WANG Wen-Quan1,*   

  1. 1 College of Tropical Agriculture and Forestry / Sanya Nanfan Research Institute, Hainan University, Sanya 572000, Hainan, China; 2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory of Conservation and Utilization of Tropical Agricultural Biological Resources, Hainan Institute of Tropical Agricultural Resources, Haikou 571101, Hainan, China; 3 Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572000, Hainan, China
  • Received:2023-09-12 Revised:2024-01-12 Accepted:2024-01-12 Published:2024-02-09
  • Supported by:
    This study was supported by the National Natural Science Foundation of China NSFC-CG Joint Fund Key Project (3181101517), the National Key Research and Development Program of China (2018YFD1000500) and the Innovation Research Project for Graduate Students in Hainan Province (Qhys2022-82).

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Abstract:

The IGT gene family is involved in the regulation of crop plant type, and LAZY belongs to the IGT subfamily. By comparing the amino acid sequences of six Arabidopsis LAZY members as ‘seeds’ in the cassava genome, a total of 8 LAZY genes were identified in cassava, among which MeLAZY1c is highly homologous to AtLAZY1, which regulates branching angles. Based on this, MeLAZY1c had the highest transcription level in the stem by qRT-PCR and pMeLAZY1c was deeply stained in the vascular bundle by GUS staining, using MeLAZY1c as the experimental materials in this study. Eight photoresponsive/regulatory elements were found in the MeLAZY1c promoter, and we subsequently found that darkness significantly inhibited the relative expression level of MeLAZY1c. At the same time, gene editing of MeLAZY1c was performed and 19 homozygous edited lineages. The phenotype of MeLAZY1c mutants was observed after seedling transplantation, compared to the SC8 wild-type, the main stems of MeLAZY1c mutants had crawing growth, and the stem skin cells at the curved part were distorted and deformed with different sizes. The number of cells at 1 mm near the ground was about 1.5 times that of cells at the far ground. In conclusion, these results indicates that MeLAZY1c plays an important role in the establishment of upright/creeping growth of cassava.

Key words: cassava, plant type regulation, LAZY, light response, crawling, gene editing

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