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Establishment of regeneration system and creation of glufosinate-resistant germplasm for early-mid maturing potato ‘Longshu 20’

JIA Xiao-Xia1,2,QI En-Fang1,2,WEN Guo-Hong1,2,*,MA Sheng1,2,HUANG Wei1,2,LYU He-Ping1,2,LI Jian-Wu1,2,QU Ya-Ying1,2,DING Ning1,2   

  1. 1 Potato Research Institute of Gansu Academy of Agricultural Sciences / Gansu Engineering Laboratory of Potato Germplasm resources Innovation, Lanzhou 730070, Gansu, China; 2 National Germplasm Resources Agricultural Experimental Station, Weiyuan 748201, Gansu, China
  • Received:2025-02-18 Revised:2025-06-01 Accepted:2025-06-01 Published:2025-06-23
  • Supported by:
    This study was supported by the Gansu Province Innovation-Driven Assistance Project (GXH20250325-3), the China Agriculture Research System of MOF and MARA (CARS-09-P06), the Special Project of Biotechnology Breeding of Gansu Academy of Agricultural Sciences(2025GAAS15) and the Project of the Central Government-Guided Local Science and Technology Development Funds (25ZYJA002-2). 

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Abstract:

To establish an efficient regeneration system for the new early-mid maturing, vegetable-use potato cultivar ‘Longshu 20’ and to develop novel germplasm resistant to glufosinate, we systematically evaluated the embryogenic callus induction efficiency and shoot regeneration capacity of test-tube plantlet stem segments cultured on MS medium supplemented with varying concentrations and combinations of 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), and gibberellic acid (GA3). The highest rates of embryogenic callus induction and shoot differentiation were observed on medium MS + 1.0 mg L?1 6-BA + 0.5 mg L?1 NAA + 4.5 mg L?1 GA3 + 3% sucrose. On day 30, the embryogenic callus induction rate reached 89.83%, while the shoot differentiation rate reached 91.81% by day 45. A Bar gene overexpression vector was constructed using recombinant DNA technology and introduced into stem segments via Agrobacterium-mediated transformation. After selection on MS medium  containing 2.0 mg L?1 phosphinothricin (PPT), six independent transgenic lines were successfully obtained. Both transgenic and non-transgenic control lines were grown and treated with commercial glufosinate-ammonium at an active ingredient content of 1271 g hm?2 during the seedling stage. Within 9 days, all non-transgenic control plants had completely withered and died, while all transgenic lines exhibited stable resistance to glufosinate-ammonium. Phenotypic analysis at maturity revealed variation among  transgenic lines in terms of tuber number per plant, yield per plant, and key quality traits. Notably, transgenic line S20-4 showed no significant differences from the non-transgenic control in tuber number, yield, or key quality parameters such as starch, crude protein, and reducing sugar content. These results demonstrate that S20-4 successfully integrates glufosinate resistance while concurrently maintaining the yield and quality traits of the recipient cultivar. The efficient regeneration system developed in this study provides a solid foundation for the genetic improvement of ‘Longshu 20’, and the transgenic line S20-4 represents a promising candidate for direct application in breeding programs targeting herbicide resistance and high yield.

Key words: potato, Longshu 20, regeneration system, Bar gene, glyphosate

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