Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (04): 578-582.

• ORIGINAL PAPERS • Previous Articles     Next Articles

The Difference of Autotoxic Compounds Content in Alfalfa Cultivars

LU Cheng12**,ZENG Zhao-Hai1**,ZHENG Shi-Zong2,QI Zhi-Qiang1,HU Yue-Gao1*   

  1. 1 Pasture Engineering Research Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094; 2 Zhejiang Institute of Hydraulics & Estuary, Hangzhou 310020, Zhejiang, China
  • Received:2006-06-21 Revised:1900-01-01 Online:2007-04-12 Published:2007-04-12
  • Contact: HU Yue-Gao

PDF

1163

Cited

1

Abstract:

Autotoxicity restricts the development of alfalfa industry. In North America researchers did some work on this field, but in China little work has been done about it. The objective of this research was to evaluate the differences of autotoxic compounds content in different alfalfa (Medicago sativa L.) cultivars by bioassay and high performance liquid chromatography. The results indicated that alfalfa leaf extracts inhibited the radicel growth of ‘vector’, and the treated radicels of ‘vector’ were shorter than that of control significantly (P<0.01). Fifty cultivars of alfalfa showed difference in aotutoxicity, and were divided into three categories based on the autotoxicity of their leaf extracts. Alfalfa ‘Key’ exhibited the least aotutoxicity, while ‘GS2001X’, ‘Super Apollo’, ‘Archer’, ‘NS00-32’, ‘Muye’, and ‘WL232HQ’ showed strong autotoxicity. Coumarine, o-coumaric acid, m-coumaric acid, and p-coumaric acid were detected in leaf extracts of alfalfa by high performance liquid chromatography.

Key words: Autotoxicity, Alfalfa, Bioassay

[1] ZHU Ping-Hui**,CHEN Ran-Ran**,YU Yang,SONG Xue-Wei,LI Hui-Qing,DU Jian-Ying,LI Qiang,DING Xiao-Dong,ZHU Yan-Ming*. Cloning of Gene GsWRKY15 Related to Alkaline Stress and Alkaline Tolerance of Transgenic Plants [J]. Acta Agron Sin, 2017, 43(09): 1319-1327.
[2] ZHAO Yang,ZHU Yan-Ming,BAI Xi,JI Wei,WU Jing,TANG Li-Li,CAI Hua. Over-expressing GsCBRLK/SCMRP from Glycine soja Enhances Alkaline Tolerance and Methionine Content in Transgenic Medicago sativa [J]. Acta Agron Sin, 2014, 40(03): 431-438.
[3] WEI Zheng-Wei,ZHU Yan-Ming,HUA Ye,CAI Hua,JI Wei,BAI Xi,WANG Zhen-Yu,WEN Yi-Dong. Transgenic Alfalfa with GsPPCK1 and Its Alkaline Tolerance Analysis [J]. Acta Agron Sin, 2013, 39(01): 68-75.
[4] WANG Zhen-Yu,CAI Hua,BAI Xi,JI Wei,LI Yong,WEI Zheng-Wei,ZHU Yan-Ming. Isolation of GsGST19 from Glycine soja and Analysis of Saline-Alkaline Tolerance for Transgenic Medicago sativa [J]. Acta Agron Sin, 2012, 38(06): 971-979.
[5] GUO Yan-Jun, NI Yu, GUO Yun-Jiang, HAN Long, TANG Hua, YU Yong-Xiong. Effect of Soil Water Deficit and High Temperature on Leaf Cuticular Waxes and Physiological Indices in Alfalfa (Medicago sativa) Leaf [J]. Acta Agron Sin, 2011, 37(05): 911-917.
[6] ZHANG Yan-Min, ZHANG Hong-Mei, XIANG Jin-Yang, GUO Xiu-Lin, LIU Zi-Hui, LI Guo-Liang, CHEN Dao-Yi. Analysis of T-DNA Flanking Sequences and Event Specific Detection of Transgenic Alfalfa with Gene BADH [J]. Acta Agron Sin, 2011, 37(03): 397-404.
[7] QIN Zhi-Hui1,2,CHAO Yue-Hui1,YANG Qing-Chuan1,*,KANG Jun-Mei1,SUN Yan3,WANG Ping. Construction and Transformation of RNAi Vector of MsZFN Gene from Alfalfa (Medicago sativa L.) [J]. Acta Agron Sin, 2010, 36(4): 596-601.
[8] ZONG Yu-Zheng,WANG Wen-Yue,HAN Qing-Fang*,DING Rui-Xia,JIA Zhi-Kuan,NIE Jun-Feng. Effects of Different Levels of Boron Fertilizer on Alfalfa Photosynthesis and Source-Sink Translocation of Soluble Carbohydrate [J]. Acta Agron Sin, 2010, 36(4): 665-672.
[9] LONG Ming-Xiu,GAO Jing-Hui,GAO Yang,HU Tian-Ming,SHI Jun-Tong,WU Zhen,. Relationship Between Dry Matter Per Plant in First Cutting and the Yield-Related Traits in Twelve Alfalfa Varieties Introduced Overseas [J]. Acta Agron Sin, 2009, 35(10): 1923-1929.
[10] AN Bao-Yan;LUO Yan;LI Jia-Rui;QIAO Wei-Hua;ZHANG Xian-Sheng;GAO Xin-Qi. Expression of a Vacuolar Na+/H+ Antiporter Gene of Alfalfa Enhances Salinity Tolerance in Transgenic Arabidopsis [J]. Acta Agron Sin, 2008, 34(04): 557-564.
[11] WANG Cheng-Zhang;HAN Jin-Feng;SHI Ying-Hua;LI Zhen-Tian;LI De-Feng. Production Performance in Alfalfa with Different Classes of Fall Dormancy [J]. Acta Agron Sin, 2008, 34(01): 133-141.
[12] ZHU Yu-Jie; FENG Li-Ping ; ; YI Peng; YING Xiao-Guang; HU Yue-Gao. A Dynamic Model Simulating Photosynthetic Production and Dry Matter Accumulation for Alfalfa (Medicago sativa L.) [J]. Acta Agron Sin, 2007, 33(10): 1682-1687.
[13] LIU Zhi-Peng; YANG Qing-Chuan; HU Tian-Ming; YAN Long-Feng and WANG Chao. Genetic Diversity of Autotetraploid Alfalfa with Different Salt-tolerant Trait Based on SSR Marker Analysis [J]. Acta Agron Sin, 2006, 32(04): 630-632.
[14] LIU Jing-Hui; ZENG Zhao-Hai; JIAO Li-Xin; HU Yue-Gao; WANG Ying; LI Hai. Intercropping of Different Silage Maize Cultivars and Alfalfa [J]. Acta Agron Sin, 2006, 32(01): 125-137.
[15] YANG Qing-Chuan;HAN Jian-Guo; SUN Yan; KAN Jun-Mei. Identification and Utilization of Molecular Marker to Salt Tolerance Gene in Alfalfa [J]. Acta Agron Sin, 2005, 31(09): 1157-1161.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd