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Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (06): 968-972.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Agrobacterium-mediated Transformation of Aloe with Trehalose Synthase Gene (otsA)

CHEN Jie12,ZHANG Jia-Xing12,YE Xing-Guo2*,HE Cong-Fen1*,DONG Yin-Mao1,ZHAO Hua1,QIN Yun-Rong1   

  1. 1 Key laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100037; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2006-09-10 Revised:1900-01-01 Online:2007-06-12 Published:2007-06-12
  • Contact: HE Cong-Fen

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Abstract:

Aloe has been deeply researched recently because of its attracting function on medical treatment, hairdressing, health protection and ediblity. Trehalose can protect organism and biomolecule, and has been applied widely in foods, cosmetics and molecule biology researches. In this experiment, aloe stems were used as explants for otsA gene transformation mediated by Agrobacterium. PCR and Southern blotting analyses demonstrated that otsA gene was successfully transferred into aloe genome with a transformation efficiency of 0.77%, and the single copy insert of the alien gene was found in about 60% positive aloe plants. Gas Chromatogram analysis showed that the content of trehalose in transgenic aloe plants was 2.934 times of that in untransformed aloe plants, revealing that otsA gene had been expressied in the transgenic aloe plants.

Key words: Aloe, Agrobacterium tumefaciens, otsA, Molecule analysis

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